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Abstract 26 HLA Analysis of the Australian Cord Blood Banks: How Diverse Are Donors?

INTRODUCTION: The network of public cord blood banks (CBB) in Australia, known as AusCord, is comprised of CBB located in Brisbane, Sydney, and Melbourne. The network stores almost 37,000 cord blood units (CBU) and has released more than 1,300 for transplantation. OBJECTIVE: The objectives of this s...

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Autores principales: Klamer, Guy, Sue, Jessica, Ko, Kap-Hyoun, Trickett, Annette, Johnson, Phillip, Elwood, Ngaire
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9446947/
http://dx.doi.org/10.1093/stcltm/szac057.026
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author Klamer, Guy
Sue, Jessica
Ko, Kap-Hyoun
Trickett, Annette
Johnson, Phillip
Elwood, Ngaire
author_facet Klamer, Guy
Sue, Jessica
Ko, Kap-Hyoun
Trickett, Annette
Johnson, Phillip
Elwood, Ngaire
author_sort Klamer, Guy
collection PubMed
description INTRODUCTION: The network of public cord blood banks (CBB) in Australia, known as AusCord, is comprised of CBB located in Brisbane, Sydney, and Melbourne. The network stores almost 37,000 cord blood units (CBU) and has released more than 1,300 for transplantation. OBJECTIVE: The objectives of this study were (1) to determine the relative diversity of HLA allele subtypes, tissue types, and haplotypes at each of the banks and between the banks and (2) to identify common tissue types and haplotypes that could be utilized for clinical research and development of third party cell therapy products. METHODS: HLA data was obtained for 36,782 CBU stored in the AusCord inventory. To standardize data format, high-resolution typing was converted to 2-digit typing. HLA allele subtypes were ranked from most to least common. A subset of Indigenous Australian and Pacific Islander HLA allele subtypes was interrogated to determine whether increased frequency correlated with declared ethnicity. Tissue types were separated into CBU that had HLA-A, HLA-B, HLA-C, and HLA-DRB1 typing (21,815 total) and CBU that did not have HLA-C typing (36,782 total). CBU tissue types were interrogated in 3 separate groups: searchable, searched, and released inventory. Haplotypes were confirmed where maternal typing was available (3,105 CBU). RESULTS: Ethnicity screening for donors and strategic location of collection sites servicing ethnic minority communities resulted in banking of Indigenous Australian and Pacific Islander HLA. At a bank and network level, there was a similar frequency of specific HLA subtypes; however, when considering the tissue types, there was vast diversity (~75% unique with 8 allele typing). Whilst there is diversity, there was also a fraction of the inventory that exhibited repetitive tissue types that could be utilized for clinical research. DISCUSSION: The study demonstrates that cord blood collections are able to boost storage of diverse tissue types in, and unique to, a multicultural population such as Australia. This finding can guide policy development and funding, as well as operational decisions at the CBB. Furthermore, CBB are well positioned to support research and development activity aimed at discovery of new applications for cord blood units through supply of GMP grade cryopreserved products exhibiting common tissue types and haplotypes.
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spelling pubmed-94469472022-09-06 Abstract 26 HLA Analysis of the Australian Cord Blood Banks: How Diverse Are Donors? Klamer, Guy Sue, Jessica Ko, Kap-Hyoun Trickett, Annette Johnson, Phillip Elwood, Ngaire Stem Cells Transl Med Cord Blood Bank Management, Administration, and Sustainability INTRODUCTION: The network of public cord blood banks (CBB) in Australia, known as AusCord, is comprised of CBB located in Brisbane, Sydney, and Melbourne. The network stores almost 37,000 cord blood units (CBU) and has released more than 1,300 for transplantation. OBJECTIVE: The objectives of this study were (1) to determine the relative diversity of HLA allele subtypes, tissue types, and haplotypes at each of the banks and between the banks and (2) to identify common tissue types and haplotypes that could be utilized for clinical research and development of third party cell therapy products. METHODS: HLA data was obtained for 36,782 CBU stored in the AusCord inventory. To standardize data format, high-resolution typing was converted to 2-digit typing. HLA allele subtypes were ranked from most to least common. A subset of Indigenous Australian and Pacific Islander HLA allele subtypes was interrogated to determine whether increased frequency correlated with declared ethnicity. Tissue types were separated into CBU that had HLA-A, HLA-B, HLA-C, and HLA-DRB1 typing (21,815 total) and CBU that did not have HLA-C typing (36,782 total). CBU tissue types were interrogated in 3 separate groups: searchable, searched, and released inventory. Haplotypes were confirmed where maternal typing was available (3,105 CBU). RESULTS: Ethnicity screening for donors and strategic location of collection sites servicing ethnic minority communities resulted in banking of Indigenous Australian and Pacific Islander HLA. At a bank and network level, there was a similar frequency of specific HLA subtypes; however, when considering the tissue types, there was vast diversity (~75% unique with 8 allele typing). Whilst there is diversity, there was also a fraction of the inventory that exhibited repetitive tissue types that could be utilized for clinical research. DISCUSSION: The study demonstrates that cord blood collections are able to boost storage of diverse tissue types in, and unique to, a multicultural population such as Australia. This finding can guide policy development and funding, as well as operational decisions at the CBB. Furthermore, CBB are well positioned to support research and development activity aimed at discovery of new applications for cord blood units through supply of GMP grade cryopreserved products exhibiting common tissue types and haplotypes. Oxford University Press 2022-09-06 /pmc/articles/PMC9446947/ http://dx.doi.org/10.1093/stcltm/szac057.026 Text en © The Author(s) 2022. Published by Oxford University Press. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Cord Blood Bank Management, Administration, and Sustainability
Klamer, Guy
Sue, Jessica
Ko, Kap-Hyoun
Trickett, Annette
Johnson, Phillip
Elwood, Ngaire
Abstract 26 HLA Analysis of the Australian Cord Blood Banks: How Diverse Are Donors?
title Abstract 26 HLA Analysis of the Australian Cord Blood Banks: How Diverse Are Donors?
title_full Abstract 26 HLA Analysis of the Australian Cord Blood Banks: How Diverse Are Donors?
title_fullStr Abstract 26 HLA Analysis of the Australian Cord Blood Banks: How Diverse Are Donors?
title_full_unstemmed Abstract 26 HLA Analysis of the Australian Cord Blood Banks: How Diverse Are Donors?
title_short Abstract 26 HLA Analysis of the Australian Cord Blood Banks: How Diverse Are Donors?
title_sort abstract 26 hla analysis of the australian cord blood banks: how diverse are donors?
topic Cord Blood Bank Management, Administration, and Sustainability
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9446947/
http://dx.doi.org/10.1093/stcltm/szac057.026
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