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Correlation of Moraxella catarrhalis macrolide susceptibility with the ability to adhere and invade human respiratory epithelial cells

Recently, the prevalence of macrolide-resistant Moraxella catarrhalis has been reported, especially among Chinese children. The fitness cost of resistance is reported to render the resistant bacteria less virulent. To investigate the correlation between macrolide susceptibility of M. catarrhalis and...

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Autores principales: Liu, Ya-li, Ding, Rui, Jia, Xin-miao, Huang, Jing-jing, Yu, Shuying, Chan, Hiu Tat, Li, Wei, Mao, Lei-li, Zhang, Li, Zhang, Xin-yao, Wu, Wei, Ni, An-ping, Xu, Ying-chun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9448378/
https://www.ncbi.nlm.nih.gov/pubmed/35904140
http://dx.doi.org/10.1080/22221751.2022.2108341
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author Liu, Ya-li
Ding, Rui
Jia, Xin-miao
Huang, Jing-jing
Yu, Shuying
Chan, Hiu Tat
Li, Wei
Mao, Lei-li
Zhang, Li
Zhang, Xin-yao
Wu, Wei
Ni, An-ping
Xu, Ying-chun
author_facet Liu, Ya-li
Ding, Rui
Jia, Xin-miao
Huang, Jing-jing
Yu, Shuying
Chan, Hiu Tat
Li, Wei
Mao, Lei-li
Zhang, Li
Zhang, Xin-yao
Wu, Wei
Ni, An-ping
Xu, Ying-chun
author_sort Liu, Ya-li
collection PubMed
description Recently, the prevalence of macrolide-resistant Moraxella catarrhalis has been reported, especially among Chinese children. The fitness cost of resistance is reported to render the resistant bacteria less virulent. To investigate the correlation between macrolide susceptibility of M. catarrhalis and pathogenicity, the whole genome of 70 M. catarrhalis isolates belonging to four clonal complexes with different macrolide susceptibilities was sequenced. The gene products were annotated with the Gene Ontology terms. Based on 46 extracted essential virulence genes, 19 representative isolates were selected to infect type II alveolar cells (A549 cells). The ability of these isolates to adhere and invade human epithelial cells and to produce cytokines was comparatively analysed. Furthermore, mice were infected with a pair of M. catarrhalis isolates with different pathogenic behaviours and macrolide susceptibilities to examine pulmonary clearance, histological findings, and the production of cytokines. The percentages of annotations for binding, metabolic process, cellular process, and cell were non-significantly different between the macrolide-resistant and macrolide-susceptible groups. The presence of uspA2, uspA2H, pilO, lbpB, lex1, modM, mboIA, and mboIB significantly differed among the four clonal complexes and macrolide susceptibility groups. Furthermore, compared with those in macrolide-susceptible isolates, the adhesion ability was stronger (P = 0.0019) and the invasion ability was weaker (P < 0.0001) in the macrolide-resistant isolates. Mouse experiments revealed that pulmonary macrophages elicit immune responses against M. catarrhalis infection by significantly upregulating the Csf2, Il4, Il13, Il1b, Il6, Tnf, and Il18. Therefore, M. catarrhalis populations exhibited diverse pathogenicity in vitro and in vivo.
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spelling pubmed-94483782022-09-07 Correlation of Moraxella catarrhalis macrolide susceptibility with the ability to adhere and invade human respiratory epithelial cells Liu, Ya-li Ding, Rui Jia, Xin-miao Huang, Jing-jing Yu, Shuying Chan, Hiu Tat Li, Wei Mao, Lei-li Zhang, Li Zhang, Xin-yao Wu, Wei Ni, An-ping Xu, Ying-chun Emerg Microbes Infect Research Article Recently, the prevalence of macrolide-resistant Moraxella catarrhalis has been reported, especially among Chinese children. The fitness cost of resistance is reported to render the resistant bacteria less virulent. To investigate the correlation between macrolide susceptibility of M. catarrhalis and pathogenicity, the whole genome of 70 M. catarrhalis isolates belonging to four clonal complexes with different macrolide susceptibilities was sequenced. The gene products were annotated with the Gene Ontology terms. Based on 46 extracted essential virulence genes, 19 representative isolates were selected to infect type II alveolar cells (A549 cells). The ability of these isolates to adhere and invade human epithelial cells and to produce cytokines was comparatively analysed. Furthermore, mice were infected with a pair of M. catarrhalis isolates with different pathogenic behaviours and macrolide susceptibilities to examine pulmonary clearance, histological findings, and the production of cytokines. The percentages of annotations for binding, metabolic process, cellular process, and cell were non-significantly different between the macrolide-resistant and macrolide-susceptible groups. The presence of uspA2, uspA2H, pilO, lbpB, lex1, modM, mboIA, and mboIB significantly differed among the four clonal complexes and macrolide susceptibility groups. Furthermore, compared with those in macrolide-susceptible isolates, the adhesion ability was stronger (P = 0.0019) and the invasion ability was weaker (P < 0.0001) in the macrolide-resistant isolates. Mouse experiments revealed that pulmonary macrophages elicit immune responses against M. catarrhalis infection by significantly upregulating the Csf2, Il4, Il13, Il1b, Il6, Tnf, and Il18. Therefore, M. catarrhalis populations exhibited diverse pathogenicity in vitro and in vivo. Taylor & Francis 2022-08-31 /pmc/articles/PMC9448378/ /pubmed/35904140 http://dx.doi.org/10.1080/22221751.2022.2108341 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Liu, Ya-li
Ding, Rui
Jia, Xin-miao
Huang, Jing-jing
Yu, Shuying
Chan, Hiu Tat
Li, Wei
Mao, Lei-li
Zhang, Li
Zhang, Xin-yao
Wu, Wei
Ni, An-ping
Xu, Ying-chun
Correlation of Moraxella catarrhalis macrolide susceptibility with the ability to adhere and invade human respiratory epithelial cells
title Correlation of Moraxella catarrhalis macrolide susceptibility with the ability to adhere and invade human respiratory epithelial cells
title_full Correlation of Moraxella catarrhalis macrolide susceptibility with the ability to adhere and invade human respiratory epithelial cells
title_fullStr Correlation of Moraxella catarrhalis macrolide susceptibility with the ability to adhere and invade human respiratory epithelial cells
title_full_unstemmed Correlation of Moraxella catarrhalis macrolide susceptibility with the ability to adhere and invade human respiratory epithelial cells
title_short Correlation of Moraxella catarrhalis macrolide susceptibility with the ability to adhere and invade human respiratory epithelial cells
title_sort correlation of moraxella catarrhalis macrolide susceptibility with the ability to adhere and invade human respiratory epithelial cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9448378/
https://www.ncbi.nlm.nih.gov/pubmed/35904140
http://dx.doi.org/10.1080/22221751.2022.2108341
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