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Cultivating epizoic diatoms provides insights into the evolution and ecology of both epibionts and hosts
Our understanding of the importance of microbiomes on large aquatic animals—such as whales, sea turtles and manatees—has advanced considerably in recent years. The latest observations indicate that epibiotic diatom communities constitute diverse, polyphyletic, and compositionally stable assemblages...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9448772/ https://www.ncbi.nlm.nih.gov/pubmed/36068258 http://dx.doi.org/10.1038/s41598-022-19064-0 |
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author | Ashworth, Matt P. Majewska, Roksana Frankovich, Thomas A. Sullivan, Michael Bosak, Sunčica Filek, Klara Van de Vijver, Bart Arendt, Michael Schwenter, Jeffrey Nel, Ronel Robinson, Nathan J. Gary, Meagan P. Theriot, Edward C. Stacy, Nicole I. Lam, Daryl W. Perrault, Justin R. Manire, Charles A. Manning, Schonna R. |
author_facet | Ashworth, Matt P. Majewska, Roksana Frankovich, Thomas A. Sullivan, Michael Bosak, Sunčica Filek, Klara Van de Vijver, Bart Arendt, Michael Schwenter, Jeffrey Nel, Ronel Robinson, Nathan J. Gary, Meagan P. Theriot, Edward C. Stacy, Nicole I. Lam, Daryl W. Perrault, Justin R. Manire, Charles A. Manning, Schonna R. |
author_sort | Ashworth, Matt P. |
collection | PubMed |
description | Our understanding of the importance of microbiomes on large aquatic animals—such as whales, sea turtles and manatees—has advanced considerably in recent years. The latest observations indicate that epibiotic diatom communities constitute diverse, polyphyletic, and compositionally stable assemblages that include both putatively obligate epizoic and generalist species. Here, we outline a successful approach to culture putatively obligate epizoic diatoms without their hosts. That some taxa can be cultured independently from their epizoic habitat raises several questions about the nature of the interaction between these animals and their epibionts. This insight allows us to propose further applications and research avenues in this growing area of study. Analyzing the DNA sequences of these cultured strains, we found that several unique diatom taxa have evolved independently to occupy epibiotic habitats. We created a library of reference sequence data for use in metabarcoding surveys of sea turtle and manatee microbiomes that will further facilitate the use of environmental DNA for studying host specificity in epizoic diatoms and the utility of diatoms as indicators of host ecology and health. We encourage the interdisciplinary community working with marine megafauna to consider including diatom sampling and diatom analysis into their routine practices. |
format | Online Article Text |
id | pubmed-9448772 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-94487722022-09-08 Cultivating epizoic diatoms provides insights into the evolution and ecology of both epibionts and hosts Ashworth, Matt P. Majewska, Roksana Frankovich, Thomas A. Sullivan, Michael Bosak, Sunčica Filek, Klara Van de Vijver, Bart Arendt, Michael Schwenter, Jeffrey Nel, Ronel Robinson, Nathan J. Gary, Meagan P. Theriot, Edward C. Stacy, Nicole I. Lam, Daryl W. Perrault, Justin R. Manire, Charles A. Manning, Schonna R. Sci Rep Article Our understanding of the importance of microbiomes on large aquatic animals—such as whales, sea turtles and manatees—has advanced considerably in recent years. The latest observations indicate that epibiotic diatom communities constitute diverse, polyphyletic, and compositionally stable assemblages that include both putatively obligate epizoic and generalist species. Here, we outline a successful approach to culture putatively obligate epizoic diatoms without their hosts. That some taxa can be cultured independently from their epizoic habitat raises several questions about the nature of the interaction between these animals and their epibionts. This insight allows us to propose further applications and research avenues in this growing area of study. Analyzing the DNA sequences of these cultured strains, we found that several unique diatom taxa have evolved independently to occupy epibiotic habitats. We created a library of reference sequence data for use in metabarcoding surveys of sea turtle and manatee microbiomes that will further facilitate the use of environmental DNA for studying host specificity in epizoic diatoms and the utility of diatoms as indicators of host ecology and health. We encourage the interdisciplinary community working with marine megafauna to consider including diatom sampling and diatom analysis into their routine practices. Nature Publishing Group UK 2022-09-06 /pmc/articles/PMC9448772/ /pubmed/36068258 http://dx.doi.org/10.1038/s41598-022-19064-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Ashworth, Matt P. Majewska, Roksana Frankovich, Thomas A. Sullivan, Michael Bosak, Sunčica Filek, Klara Van de Vijver, Bart Arendt, Michael Schwenter, Jeffrey Nel, Ronel Robinson, Nathan J. Gary, Meagan P. Theriot, Edward C. Stacy, Nicole I. Lam, Daryl W. Perrault, Justin R. Manire, Charles A. Manning, Schonna R. Cultivating epizoic diatoms provides insights into the evolution and ecology of both epibionts and hosts |
title | Cultivating epizoic diatoms provides insights into the evolution and ecology of both epibionts and hosts |
title_full | Cultivating epizoic diatoms provides insights into the evolution and ecology of both epibionts and hosts |
title_fullStr | Cultivating epizoic diatoms provides insights into the evolution and ecology of both epibionts and hosts |
title_full_unstemmed | Cultivating epizoic diatoms provides insights into the evolution and ecology of both epibionts and hosts |
title_short | Cultivating epizoic diatoms provides insights into the evolution and ecology of both epibionts and hosts |
title_sort | cultivating epizoic diatoms provides insights into the evolution and ecology of both epibionts and hosts |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9448772/ https://www.ncbi.nlm.nih.gov/pubmed/36068258 http://dx.doi.org/10.1038/s41598-022-19064-0 |
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