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Extracellular vesicles from colorectal cancer cells promote metastasis via the NOD1 signalling pathway

Pattern‐recognition receptors (PRRs) have been shown to promote tumour metastasis via sensing tumour cell‐derived small extracellular vesicles (EVs). Nucleotide‐binding oligomerisation domain 1 (NOD1), a cytoplasmic PRR, plays a role in colorectal cancer (CRC) by detecting bacterial products. Howeve...

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Autores principales: Wei, Xiduan, Ye, Jingjia, Pei, Yameng, Wang, Chunting, Yang, Hongzhen, Tian, Jingyuan, Si, Guangxu, Ma, Yao, Wang, Kun, Liu, Gang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9448875/
https://www.ncbi.nlm.nih.gov/pubmed/36068649
http://dx.doi.org/10.1002/jev2.12264
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author Wei, Xiduan
Ye, Jingjia
Pei, Yameng
Wang, Chunting
Yang, Hongzhen
Tian, Jingyuan
Si, Guangxu
Ma, Yao
Wang, Kun
Liu, Gang
author_facet Wei, Xiduan
Ye, Jingjia
Pei, Yameng
Wang, Chunting
Yang, Hongzhen
Tian, Jingyuan
Si, Guangxu
Ma, Yao
Wang, Kun
Liu, Gang
author_sort Wei, Xiduan
collection PubMed
description Pattern‐recognition receptors (PRRs) have been shown to promote tumour metastasis via sensing tumour cell‐derived small extracellular vesicles (EVs). Nucleotide‐binding oligomerisation domain 1 (NOD1), a cytoplasmic PRR, plays a role in colorectal cancer (CRC) by detecting bacterial products. However, the precise mechanisms underlying the effects of NOD1, following identification of CRC cell‐derived EVs (CRC‐EVs), to potentiate CRC liver metastasis (CRC‐LM), remain poorly understood. Here, we demonstrate that CRC‐EVs activate NOD1 in macrophages to initiate secretion of inflammatory cytokines and chemokines. NOD1‐activated macrophages also promote CRC cell migration, while in a murine model of liver metastasis (LM), NOD1‐deficient mice exhibit reduced metastasis following CRC‐EV treatment. Furthermore, cell division cycle 42 (CDC42), a small Rho guanosine‐5′‐triphosphate (GTP)ase, is delivered by CRC‐EVs into macrophages where it activates NOD1. In addition, EVs from the plasma of patients with CRC‐LM mediate NOD1 activation in human peripheral blood mononuclear cells. Moreover, high NOD1 expression in tumour tissues is associated with poor prognosis of CRC‐LM. Our findings suggest that CRC‐EVs activate NOD1 to promote tumour metastasis, thus, NOD1 may serve as a potential target in the diagnosis and treatment of CRC‐LM.
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spelling pubmed-94488752022-09-09 Extracellular vesicles from colorectal cancer cells promote metastasis via the NOD1 signalling pathway Wei, Xiduan Ye, Jingjia Pei, Yameng Wang, Chunting Yang, Hongzhen Tian, Jingyuan Si, Guangxu Ma, Yao Wang, Kun Liu, Gang J Extracell Vesicles Research Articles Pattern‐recognition receptors (PRRs) have been shown to promote tumour metastasis via sensing tumour cell‐derived small extracellular vesicles (EVs). Nucleotide‐binding oligomerisation domain 1 (NOD1), a cytoplasmic PRR, plays a role in colorectal cancer (CRC) by detecting bacterial products. However, the precise mechanisms underlying the effects of NOD1, following identification of CRC cell‐derived EVs (CRC‐EVs), to potentiate CRC liver metastasis (CRC‐LM), remain poorly understood. Here, we demonstrate that CRC‐EVs activate NOD1 in macrophages to initiate secretion of inflammatory cytokines and chemokines. NOD1‐activated macrophages also promote CRC cell migration, while in a murine model of liver metastasis (LM), NOD1‐deficient mice exhibit reduced metastasis following CRC‐EV treatment. Furthermore, cell division cycle 42 (CDC42), a small Rho guanosine‐5′‐triphosphate (GTP)ase, is delivered by CRC‐EVs into macrophages where it activates NOD1. In addition, EVs from the plasma of patients with CRC‐LM mediate NOD1 activation in human peripheral blood mononuclear cells. Moreover, high NOD1 expression in tumour tissues is associated with poor prognosis of CRC‐LM. Our findings suggest that CRC‐EVs activate NOD1 to promote tumour metastasis, thus, NOD1 may serve as a potential target in the diagnosis and treatment of CRC‐LM. John Wiley and Sons Inc. 2022-09-06 2022-09 /pmc/articles/PMC9448875/ /pubmed/36068649 http://dx.doi.org/10.1002/jev2.12264 Text en © 2022 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Wei, Xiduan
Ye, Jingjia
Pei, Yameng
Wang, Chunting
Yang, Hongzhen
Tian, Jingyuan
Si, Guangxu
Ma, Yao
Wang, Kun
Liu, Gang
Extracellular vesicles from colorectal cancer cells promote metastasis via the NOD1 signalling pathway
title Extracellular vesicles from colorectal cancer cells promote metastasis via the NOD1 signalling pathway
title_full Extracellular vesicles from colorectal cancer cells promote metastasis via the NOD1 signalling pathway
title_fullStr Extracellular vesicles from colorectal cancer cells promote metastasis via the NOD1 signalling pathway
title_full_unstemmed Extracellular vesicles from colorectal cancer cells promote metastasis via the NOD1 signalling pathway
title_short Extracellular vesicles from colorectal cancer cells promote metastasis via the NOD1 signalling pathway
title_sort extracellular vesicles from colorectal cancer cells promote metastasis via the nod1 signalling pathway
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9448875/
https://www.ncbi.nlm.nih.gov/pubmed/36068649
http://dx.doi.org/10.1002/jev2.12264
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