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Development of a rapid and accurate CRISPR/Cas13-based diagnostic test for GII.4 norovirus infection
Genogroup II genotype 4 (GII.4) norovirus causes acute gastroenteritis in children, and its infection is more severe than that of other genotypes. Early and precise detection and treatment are critical for controlling its spread and reducing the severity of infection. In this study, a rapid and effi...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9449452/ https://www.ncbi.nlm.nih.gov/pubmed/36090107 http://dx.doi.org/10.3389/fmicb.2022.912315 |
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author | Duan, Lei Yang, Xiaohan Zhan, Wenli Tang, Yuan Wei, Mengru Chen, Keyi Liu, Pan Xie, Jia Zhang, Changbin Zhao, Hongyu Luo, Mingyong |
author_facet | Duan, Lei Yang, Xiaohan Zhan, Wenli Tang, Yuan Wei, Mengru Chen, Keyi Liu, Pan Xie, Jia Zhang, Changbin Zhao, Hongyu Luo, Mingyong |
author_sort | Duan, Lei |
collection | PubMed |
description | Genogroup II genotype 4 (GII.4) norovirus causes acute gastroenteritis in children, and its infection is more severe than that of other genotypes. Early and precise detection and treatment are critical for controlling its spread and reducing the severity of infection. In this study, a rapid and efficient isothermal assay for the GII.4 norovirus detection (GII.4-CRISPR detection) was developed based on the CRISPR/Cas13a system. The assay can be applied without expensive instrumentation, and the results can be read via both fluorescence and lateral flow strip (LFS). The analytical sensitivity of this assay was 5 copies/reaction, and there was no cross-reaction with other genotypes of norovirus or other clinically common pathogens. There was a coincidence rate of 100% between our assay and commercial quantitative polymerase chain reaction. GII.4-CRISPR detection improves upon the shortcomings of some previously established molecular methods of detection, particularly with regard to accessibility. It provides an alternative tool for outbreak control and early diagnosis of GII.4 norovirus infection. |
format | Online Article Text |
id | pubmed-9449452 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94494522022-09-08 Development of a rapid and accurate CRISPR/Cas13-based diagnostic test for GII.4 norovirus infection Duan, Lei Yang, Xiaohan Zhan, Wenli Tang, Yuan Wei, Mengru Chen, Keyi Liu, Pan Xie, Jia Zhang, Changbin Zhao, Hongyu Luo, Mingyong Front Microbiol Microbiology Genogroup II genotype 4 (GII.4) norovirus causes acute gastroenteritis in children, and its infection is more severe than that of other genotypes. Early and precise detection and treatment are critical for controlling its spread and reducing the severity of infection. In this study, a rapid and efficient isothermal assay for the GII.4 norovirus detection (GII.4-CRISPR detection) was developed based on the CRISPR/Cas13a system. The assay can be applied without expensive instrumentation, and the results can be read via both fluorescence and lateral flow strip (LFS). The analytical sensitivity of this assay was 5 copies/reaction, and there was no cross-reaction with other genotypes of norovirus or other clinically common pathogens. There was a coincidence rate of 100% between our assay and commercial quantitative polymerase chain reaction. GII.4-CRISPR detection improves upon the shortcomings of some previously established molecular methods of detection, particularly with regard to accessibility. It provides an alternative tool for outbreak control and early diagnosis of GII.4 norovirus infection. Frontiers Media S.A. 2022-08-24 /pmc/articles/PMC9449452/ /pubmed/36090107 http://dx.doi.org/10.3389/fmicb.2022.912315 Text en Copyright © 2022 Duan, Yang, Zhan, Tang, Wei, Chen, Liu, Xie, Zhang, Zhao and Luo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Duan, Lei Yang, Xiaohan Zhan, Wenli Tang, Yuan Wei, Mengru Chen, Keyi Liu, Pan Xie, Jia Zhang, Changbin Zhao, Hongyu Luo, Mingyong Development of a rapid and accurate CRISPR/Cas13-based diagnostic test for GII.4 norovirus infection |
title | Development of a rapid and accurate CRISPR/Cas13-based diagnostic test for GII.4 norovirus infection |
title_full | Development of a rapid and accurate CRISPR/Cas13-based diagnostic test for GII.4 norovirus infection |
title_fullStr | Development of a rapid and accurate CRISPR/Cas13-based diagnostic test for GII.4 norovirus infection |
title_full_unstemmed | Development of a rapid and accurate CRISPR/Cas13-based diagnostic test for GII.4 norovirus infection |
title_short | Development of a rapid and accurate CRISPR/Cas13-based diagnostic test for GII.4 norovirus infection |
title_sort | development of a rapid and accurate crispr/cas13-based diagnostic test for gii.4 norovirus infection |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9449452/ https://www.ncbi.nlm.nih.gov/pubmed/36090107 http://dx.doi.org/10.3389/fmicb.2022.912315 |
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