Differentiation of beta-like cells from human induced pluripotent stem cell-derived pancreatic progenitor organoids

Human induced pluripotent stem cells (hiPSCs) and organoids are important for modeling human development and disease in vitro. In this study, we describe a protocol to differentiate hiPSC toward pancreatic progenitor (PP) organoids and beta-like cells. We detail the expansion and seeding of hiPSC, P...

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Detalles Bibliográficos
Autores principales: Pedraza-Arevalo, Sergio, Cujba, Ana-Maria, Alvarez-Fallas, Mario Enrique, Sancho, Rocio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9449863/
https://www.ncbi.nlm.nih.gov/pubmed/36092820
http://dx.doi.org/10.1016/j.xpro.2022.101656
Descripción
Sumario:Human induced pluripotent stem cells (hiPSCs) and organoids are important for modeling human development and disease in vitro. In this study, we describe a protocol to differentiate hiPSC toward pancreatic progenitor (PP) organoids and beta-like cells. We detail the expansion and seeding of hiPSC, PP differentiation, organoid expansion, and the differentiation of PP into beta cells. Upon differentiation, organoids contained beta, delta, and alpha cells. For complete details on the use and execution of this protocol, please refer to Cujba et al. (2022).

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