Differentiation of beta-like cells from human induced pluripotent stem cell-derived pancreatic progenitor organoids

Human induced pluripotent stem cells (hiPSCs) and organoids are important for modeling human development and disease in vitro. In this study, we describe a protocol to differentiate hiPSC toward pancreatic progenitor (PP) organoids and beta-like cells. We detail the expansion and seeding of hiPSC, P...

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Detalles Bibliográficos
Autores principales: Pedraza-Arevalo, Sergio, Cujba, Ana-Maria, Alvarez-Fallas, Mario Enrique, Sancho, Rocio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9449863/
https://www.ncbi.nlm.nih.gov/pubmed/36092820
http://dx.doi.org/10.1016/j.xpro.2022.101656
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author Pedraza-Arevalo, Sergio
Cujba, Ana-Maria
Alvarez-Fallas, Mario Enrique
Sancho, Rocio
author_facet Pedraza-Arevalo, Sergio
Cujba, Ana-Maria
Alvarez-Fallas, Mario Enrique
Sancho, Rocio
author_sort Pedraza-Arevalo, Sergio
collection PubMed
description Human induced pluripotent stem cells (hiPSCs) and organoids are important for modeling human development and disease in vitro. In this study, we describe a protocol to differentiate hiPSC toward pancreatic progenitor (PP) organoids and beta-like cells. We detail the expansion and seeding of hiPSC, PP differentiation, organoid expansion, and the differentiation of PP into beta cells. Upon differentiation, organoids contained beta, delta, and alpha cells. For complete details on the use and execution of this protocol, please refer to Cujba et al. (2022).
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spelling pubmed-94498632022-09-08 Differentiation of beta-like cells from human induced pluripotent stem cell-derived pancreatic progenitor organoids Pedraza-Arevalo, Sergio Cujba, Ana-Maria Alvarez-Fallas, Mario Enrique Sancho, Rocio STAR Protoc Protocol Human induced pluripotent stem cells (hiPSCs) and organoids are important for modeling human development and disease in vitro. In this study, we describe a protocol to differentiate hiPSC toward pancreatic progenitor (PP) organoids and beta-like cells. We detail the expansion and seeding of hiPSC, PP differentiation, organoid expansion, and the differentiation of PP into beta cells. Upon differentiation, organoids contained beta, delta, and alpha cells. For complete details on the use and execution of this protocol, please refer to Cujba et al. (2022). Elsevier 2022-08-30 /pmc/articles/PMC9449863/ /pubmed/36092820 http://dx.doi.org/10.1016/j.xpro.2022.101656 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Pedraza-Arevalo, Sergio
Cujba, Ana-Maria
Alvarez-Fallas, Mario Enrique
Sancho, Rocio
Differentiation of beta-like cells from human induced pluripotent stem cell-derived pancreatic progenitor organoids
title Differentiation of beta-like cells from human induced pluripotent stem cell-derived pancreatic progenitor organoids
title_full Differentiation of beta-like cells from human induced pluripotent stem cell-derived pancreatic progenitor organoids
title_fullStr Differentiation of beta-like cells from human induced pluripotent stem cell-derived pancreatic progenitor organoids
title_full_unstemmed Differentiation of beta-like cells from human induced pluripotent stem cell-derived pancreatic progenitor organoids
title_short Differentiation of beta-like cells from human induced pluripotent stem cell-derived pancreatic progenitor organoids
title_sort differentiation of beta-like cells from human induced pluripotent stem cell-derived pancreatic progenitor organoids
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9449863/
https://www.ncbi.nlm.nih.gov/pubmed/36092820
http://dx.doi.org/10.1016/j.xpro.2022.101656
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