Effect of heat inactivation and bulk lysis on real-time reverse transcription PCR detection of the SARS-COV-2: an experimental study

OBJECTIVE: This study aimed to investigate the effect of heat inactivation and chemical bulklysis on SARS-CoV-2 detection. RESULTS: About 6.2% (5/80) of samples were changed to negative results in heat inactivation at 60 °C and about 8.7% (7/80) of samples were changed to negative in heat inactivati...

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Autores principales: Leta, Dereje, Gutema, Gadissa, Hagos, Gebremedhin Gebremichael, Diriba, Regasa, Bulti, Gutema, Sura, Tolawak, Ayana, Desta, Chala, Dawit, Lenjiso, Boki, Bulti, Jaleta, Abdella, Saro, Tola, Habteyes Hailu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9449930/
https://www.ncbi.nlm.nih.gov/pubmed/36071470
http://dx.doi.org/10.1186/s13104-022-06184-z
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author Leta, Dereje
Gutema, Gadissa
Hagos, Gebremedhin Gebremichael
Diriba, Regasa
Bulti, Gutema
Sura, Tolawak
Ayana, Desta
Chala, Dawit
Lenjiso, Boki
Bulti, Jaleta
Abdella, Saro
Tola, Habteyes Hailu
author_facet Leta, Dereje
Gutema, Gadissa
Hagos, Gebremedhin Gebremichael
Diriba, Regasa
Bulti, Gutema
Sura, Tolawak
Ayana, Desta
Chala, Dawit
Lenjiso, Boki
Bulti, Jaleta
Abdella, Saro
Tola, Habteyes Hailu
author_sort Leta, Dereje
collection PubMed
description OBJECTIVE: This study aimed to investigate the effect of heat inactivation and chemical bulklysis on SARS-CoV-2 detection. RESULTS: About 6.2% (5/80) of samples were changed to negative results in heat inactivation at 60 °C and about 8.7% (7/80) of samples were changed to negative in heat inactivation at 100 °C. The Ct values of heat-inactivated samples (at 60 °C, at 100 °C, and bulk lysis) were significantly different from the temperature at 56 °C. The effect of heat on Ct value should be considered when interpreting diagnostic PCR results from clinical samples which could have an initial low virus concentration. The efficacy of heat-inactivation varies greatly depending on temperature and duration. Local validation of heat-inactivation and its effects is therefore essential for molecular testing.
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spelling pubmed-94499302022-09-07 Effect of heat inactivation and bulk lysis on real-time reverse transcription PCR detection of the SARS-COV-2: an experimental study Leta, Dereje Gutema, Gadissa Hagos, Gebremedhin Gebremichael Diriba, Regasa Bulti, Gutema Sura, Tolawak Ayana, Desta Chala, Dawit Lenjiso, Boki Bulti, Jaleta Abdella, Saro Tola, Habteyes Hailu BMC Res Notes Research Note OBJECTIVE: This study aimed to investigate the effect of heat inactivation and chemical bulklysis on SARS-CoV-2 detection. RESULTS: About 6.2% (5/80) of samples were changed to negative results in heat inactivation at 60 °C and about 8.7% (7/80) of samples were changed to negative in heat inactivation at 100 °C. The Ct values of heat-inactivated samples (at 60 °C, at 100 °C, and bulk lysis) were significantly different from the temperature at 56 °C. The effect of heat on Ct value should be considered when interpreting diagnostic PCR results from clinical samples which could have an initial low virus concentration. The efficacy of heat-inactivation varies greatly depending on temperature and duration. Local validation of heat-inactivation and its effects is therefore essential for molecular testing. BioMed Central 2022-09-07 /pmc/articles/PMC9449930/ /pubmed/36071470 http://dx.doi.org/10.1186/s13104-022-06184-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Note
Leta, Dereje
Gutema, Gadissa
Hagos, Gebremedhin Gebremichael
Diriba, Regasa
Bulti, Gutema
Sura, Tolawak
Ayana, Desta
Chala, Dawit
Lenjiso, Boki
Bulti, Jaleta
Abdella, Saro
Tola, Habteyes Hailu
Effect of heat inactivation and bulk lysis on real-time reverse transcription PCR detection of the SARS-COV-2: an experimental study
title Effect of heat inactivation and bulk lysis on real-time reverse transcription PCR detection of the SARS-COV-2: an experimental study
title_full Effect of heat inactivation and bulk lysis on real-time reverse transcription PCR detection of the SARS-COV-2: an experimental study
title_fullStr Effect of heat inactivation and bulk lysis on real-time reverse transcription PCR detection of the SARS-COV-2: an experimental study
title_full_unstemmed Effect of heat inactivation and bulk lysis on real-time reverse transcription PCR detection of the SARS-COV-2: an experimental study
title_short Effect of heat inactivation and bulk lysis on real-time reverse transcription PCR detection of the SARS-COV-2: an experimental study
title_sort effect of heat inactivation and bulk lysis on real-time reverse transcription pcr detection of the sars-cov-2: an experimental study
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9449930/
https://www.ncbi.nlm.nih.gov/pubmed/36071470
http://dx.doi.org/10.1186/s13104-022-06184-z
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