Cargando…

Effect of heat inactivation and bulk lysis on real-time reverse transcription PCR detection of the SARS-COV-2: an experimental study

OBJECTIVE: This study aimed to investigate the effect of heat inactivation and chemical bulklysis on SARS-CoV-2 detection. RESULTS: About 6.2% (5/80) of samples were changed to negative results in heat inactivation at 60 °C and about 8.7% (7/80) of samples were changed to negative in heat inactivati...

Descripción completa

Detalles Bibliográficos
Autores principales:	Leta, Dereje, Gutema, Gadissa, Hagos, Gebremedhin Gebremichael, Diriba, Regasa, Bulti, Gutema, Sura, Tolawak, Ayana, Desta, Chala, Dawit, Lenjiso, Boki, Bulti, Jaleta, Abdella, Saro, Tola, Habteyes Hailu
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2022
Materias:	Research Note
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9449930/ https://www.ncbi.nlm.nih.gov/pubmed/36071470 http://dx.doi.org/10.1186/s13104-022-06184-z

Ejemplares similares

Positivity rate, trend and associated risk factors of mother-to-child transmission of HIV among HIV-exposed infants
por: Gutema, Gadissa, et al.
Publicado: (2023)

Prevalence and factors associated with inconsistent condom use among female sex workers in Ethiopia: findings from the national biobehavioral survey, 2020
por: Rameto, Muhammed Ahmed, et al.
Publicado: (2023)

The effect of simulation-based training in non-physician anesthetists in Tigray region, Ethiopia
por: Etanaa, Naod Bulti, et al.
Publicado: (2020)

Magnitude and Determinants of Syphilis and HIV Co-Infection Among Female Sex Workers in Ethiopia: Evidence from Respondent Driven Samples, 2019–2020
por: Barba, Ammar, et al.
Publicado: (2022)

Prevalence of syphilis and associated factors among female sex workers in Ethiopia: findings from a multilevel analysis of a national bio-behavioral survey
por: Tura, Jaleta Bulti, et al.
Publicado: (2023)

Incidence of Hepatotoxicity and Factors Associated During Highly Active Antiretroviral Therapy in People Living with HIV in Ethiopia: A Prospective Cohort Study
por: Gebremicael, Gebremedhin, et al.
Publicado: (2021)

Performance of point-of-care urine test in diagnosing tuberculosis suspects with and without HIV infection in selected peripheral health settings of Addis Ababa, Ethiopia
por: Sahle, Selam Niguse, et al.
Publicado: (2017)

Epidemiology of extensively drug-resistant tuberculosis among patients with multidrug-resistant tuberculosis: A systematic review and meta-analysis
por: Diriba, Getu, et al.
Publicado: (2023)

Cropping Up Crisis at the Nexus Between COVID-19 and Antimicrobial Resistance (AMR) in Africa: A Scoping Review and Synthesis of Early Evidence
por: Gutema, Girma, et al.
Publicado: (2022)

Prevalence and factors associated with hepatitis B and C virus infections among female Sex workers in Ethiopia: Results of the national biobehavioral Survey, 2020
por: Bedassa, Birra Bejiga, et al.
Publicado: (2022)

Correction: Prevalence and factors associated with hepatitis B and C virus infections among female Sex workers in Ethiopia: Results of the national biobehavioral Survey, 2020
por: Bedassa, Birra Bejiga, et al.
Publicado: (2023)

Effect of sample management on quantitative HIV-1 viral load measurement at Saint Paul’s Hospital Millennium Medical College, Addis Ababa, Ethiopia
por: Gutema, Gadissa, et al.
Publicado: (2022)

Performances of four Nucleic Acid Amplification Tests for the identification of SARS-CoV-2 in Ethiopia
por: Hailemariam, Belete Woldesemayat, et al.
Publicado: (2022)

Risk communication during novel corona-virus disease 2019 pandemic in low health service coverage setup: The case of Ethiopia
por: Tola, Habteyes Hailu
Publicado: (2020)

Determinants of sexually transmitted infections among female sex workers in Ethiopia: a count regression model approach
por: Wariso, Feyiso Bati, et al.
Publicado: (2023)

Drug resistance and its risk factors among extrapulmonary tuberculosis in Ethiopia: A systematic review and meta-analysis
por: Diriba, Getu, et al.
Publicado: (2021)

Bacteriologically confirmed extrapulmonary tuberculosis and the associated risk factors among extrapulmonary tuberculosis suspected patients in Ethiopia: A systematic review and meta-analysis
por: Diriba, Getu, et al.
Publicado: (2022)

The Effect of Organic Solid Waste Compost on Soil Properties, Growth, and Yield of Swiss Chard Crop (Beta vulgaris L.)
por: Kebede, Temesgen, et al.
Publicado: (2023)

Effect of heat inactivation for the detection of severe acute respiratory syndrome-corona virus-2 (SARS-CoV-2) with reverse transcription real time polymerase chain reaction (rRT-PCR): evidence from Ethiopian study
por: Woldesemayat, Belete, et al.
Publicado: (2022)

High Inequality and Slow Services Improvement in Newborn and Child Health Interventions in Ethiopia
por: Tola, Habteyes Hailu, et al.
Publicado: (2020)

Predictors of adverse perinatal outcome among women who gave birth at Medical Center of Southwest Ethiopia: a retrospective cohort study
por: Jaleta, Debela Dereje, et al.
Publicado: (2022)

Enrollment in community based health insurance program and the associated factors among households in Boricha district, Sidama Zone, Southern Ethiopia; a cross-sectional study
por: Nageso, Dawit, et al.
Publicado: (2020)

Effects of sociodemographic characteristics and patients’ health beliefs on tuberculosis treatment adherence in Ethiopia: a structural equation modelling approach
por: Tola, Habteyes Hailu, et al.
Publicado: (2017)

Asymptomatic malaria and nurturing factors in lowlands of Ethiopia: A community based cross-sectional study
por: Mengesha, Endale, et al.
Publicado: (2022)

A survey of cricoid pressure application in a single institution in Ethiopia
por: Etanaa, Naod Bulti, et al.
Publicado: (2019)

AMMI and GGE biplot analysis of genotype by environment interaction and yield stability in early maturing cowpea [Vigna unguiculata (L) Walp] landraces in Ethiopia
por: Kindie, Yirga, et al.
Publicado: (2021)

Virologic and immunologic outcome of HAART in Human Immunodeficiency Virus (HIV)-1 infected patients with and without tuberculosis (TB) and latent TB infection (LTBI) in Addis Ababa, Ethiopia
por: Kassa, Desta, et al.
Publicado: (2013)

Determinants of Occupational Health and Safety Outcomes Among Sanitation Workers Across Worldwide: A Systematic Review on Cross-Sectional Studies
por: Tolera, Sina Temesgen, et al.
Publicado: (2023)

Effect of community based nutritional education on knowledge, attitude and compliance to IFA supplementation among pregnant women in rural areas of southwest Ethiopia: a quasi experimental study
por: Abdisa, Diriba Kumara, et al.
Publicado: (2023)

Perinatal outcomes of women with hypertensive disorders of pregnancy in Jimma Medical Center, southwest Ethiopia: Retrospective cohort study
por: Jaleta, Debela Dereje, et al.
Publicado: (2021)

Optimal Combinations of Control Strategies and Cost-Effectiveness Analysis of Dynamics of Endemic Malaria Transmission Model
por: Edossa, Dereje Gutema, et al.
Publicado: (2023)

Tuberculosis Treatment Non-Adherence and Lost to Follow Up among TB Patients with or without HIV in Developing Countries: A Systematic Review
por: TOLA, Habteyes Hailu, et al.
Publicado: (2015)

Occurrence of asymptomatic malaria infection and living conditions in the lowlands of Ethiopia: a community-based cross-sectional study
por: Goshu, Endale Mengesha, et al.
Publicado: (2022)

Green trees preservation: A sustainable source of valuable mushrooms for Ethiopian local communities
por: Dejene, Tatek, et al.
Publicado: (2023)

The Effect of Psychosocial Factors and Patients' Perception of Tuberculosis Treatment Non-Adherence in Addis Ababa, Ethiopia
por: Tola, Habteyes Hailu, et al.
Publicado: (2017)

Longitudinal profile of antibody response to SARS-CoV-2 in patients with COVID-19 in a setting from Sub–Saharan Africa: A prospective longitudinal study
por: Gebrecherkos, Teklay, et al.
Publicado: (2022)

Prevalence of tuberculosis, multidrug resistant tuberculosis and associated risk factors among smear negative presumptive pulmonary tuberculosis patients in Addis Ababa, Ethiopia
por: Sinshaw, Waganeh, et al.
Publicado: (2019)

Survival Status and Predictors of Mortality Among Colorectal Cancer Patients in Tikur Anbessa Specialized Hospital, Addis Ababa, Ethiopia: A Retrospective Followup Study
por: Atinafu, Bantalem Tilaye, et al.
Publicado: (2020)

Engineering the morphology of palladium nanostructures to tune their electrocatalytic activity in formic acid oxidation reactions
por: Pramanick, Bulti, et al.
Publicado: (2020)

Correction: Engineering the morphology of palladium nanostructures to tune their electrocatalytic activity in formic acid oxidation reactions
por: Pramanick, Bulti, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_000ubikptkerfot5s3b9eame7k