Cargando…
Affimers and nanobodies as molecular probes and their applications in imaging
Antibodies are the most widely used, traditional tool for labelling molecules in cells. In the past five to ten years, many new labelling tools have been developed with significant advantages over the traditional antibody. Here, we focus on nanobodies and the non-antibody binding scaffold proteins c...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9450889/ https://www.ncbi.nlm.nih.gov/pubmed/35848463 http://dx.doi.org/10.1242/jcs.259168 |
_version_ | 1784784626107023360 |
---|---|
author | Cordell, Paul Carrington, Glenn Curd, Alistair Parker, Francine Tomlinson, Darren Peckham, Michelle |
author_facet | Cordell, Paul Carrington, Glenn Curd, Alistair Parker, Francine Tomlinson, Darren Peckham, Michelle |
author_sort | Cordell, Paul |
collection | PubMed |
description | Antibodies are the most widely used, traditional tool for labelling molecules in cells. In the past five to ten years, many new labelling tools have been developed with significant advantages over the traditional antibody. Here, we focus on nanobodies and the non-antibody binding scaffold proteins called Affimers. We explain how they are generated, selected and produced, and we describe how their small size, high binding affinity and specificity provides them with many advantages compared to antibodies. Of particular importance, their small size enables them to better penetrate dense cytoskeletal regions within cells, as well as tissues, providing them with specific advantage for super-resolution imaging, as they place the fluorophore with a few nanometres of the target protein being imaged. We expect these novel tools to be of broad interest to many cell biologists and anticipate them becoming the tools of choice for super-resolution imaging. |
format | Online Article Text |
id | pubmed-9450889 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The Company of Biologists Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-94508892022-10-25 Affimers and nanobodies as molecular probes and their applications in imaging Cordell, Paul Carrington, Glenn Curd, Alistair Parker, Francine Tomlinson, Darren Peckham, Michelle J Cell Sci Review Antibodies are the most widely used, traditional tool for labelling molecules in cells. In the past five to ten years, many new labelling tools have been developed with significant advantages over the traditional antibody. Here, we focus on nanobodies and the non-antibody binding scaffold proteins called Affimers. We explain how they are generated, selected and produced, and we describe how their small size, high binding affinity and specificity provides them with many advantages compared to antibodies. Of particular importance, their small size enables them to better penetrate dense cytoskeletal regions within cells, as well as tissues, providing them with specific advantage for super-resolution imaging, as they place the fluorophore with a few nanometres of the target protein being imaged. We expect these novel tools to be of broad interest to many cell biologists and anticipate them becoming the tools of choice for super-resolution imaging. The Company of Biologists Ltd 2022-07-18 /pmc/articles/PMC9450889/ /pubmed/35848463 http://dx.doi.org/10.1242/jcs.259168 Text en © 2022. Published by The Company of Biologists Ltd https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Review Cordell, Paul Carrington, Glenn Curd, Alistair Parker, Francine Tomlinson, Darren Peckham, Michelle Affimers and nanobodies as molecular probes and their applications in imaging |
title | Affimers and nanobodies as molecular probes and their applications in imaging |
title_full | Affimers and nanobodies as molecular probes and their applications in imaging |
title_fullStr | Affimers and nanobodies as molecular probes and their applications in imaging |
title_full_unstemmed | Affimers and nanobodies as molecular probes and their applications in imaging |
title_short | Affimers and nanobodies as molecular probes and their applications in imaging |
title_sort | affimers and nanobodies as molecular probes and their applications in imaging |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9450889/ https://www.ncbi.nlm.nih.gov/pubmed/35848463 http://dx.doi.org/10.1242/jcs.259168 |
work_keys_str_mv | AT cordellpaul affimersandnanobodiesasmolecularprobesandtheirapplicationsinimaging AT carringtonglenn affimersandnanobodiesasmolecularprobesandtheirapplicationsinimaging AT curdalistair affimersandnanobodiesasmolecularprobesandtheirapplicationsinimaging AT parkerfrancine affimersandnanobodiesasmolecularprobesandtheirapplicationsinimaging AT tomlinsondarren affimersandnanobodiesasmolecularprobesandtheirapplicationsinimaging AT peckhammichelle affimersandnanobodiesasmolecularprobesandtheirapplicationsinimaging |