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Affimers and nanobodies as molecular probes and their applications in imaging

Antibodies are the most widely used, traditional tool for labelling molecules in cells. In the past five to ten years, many new labelling tools have been developed with significant advantages over the traditional antibody. Here, we focus on nanobodies and the non-antibody binding scaffold proteins c...

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Autores principales: Cordell, Paul, Carrington, Glenn, Curd, Alistair, Parker, Francine, Tomlinson, Darren, Peckham, Michelle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9450889/
https://www.ncbi.nlm.nih.gov/pubmed/35848463
http://dx.doi.org/10.1242/jcs.259168
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author Cordell, Paul
Carrington, Glenn
Curd, Alistair
Parker, Francine
Tomlinson, Darren
Peckham, Michelle
author_facet Cordell, Paul
Carrington, Glenn
Curd, Alistair
Parker, Francine
Tomlinson, Darren
Peckham, Michelle
author_sort Cordell, Paul
collection PubMed
description Antibodies are the most widely used, traditional tool for labelling molecules in cells. In the past five to ten years, many new labelling tools have been developed with significant advantages over the traditional antibody. Here, we focus on nanobodies and the non-antibody binding scaffold proteins called Affimers. We explain how they are generated, selected and produced, and we describe how their small size, high binding affinity and specificity provides them with many advantages compared to antibodies. Of particular importance, their small size enables them to better penetrate dense cytoskeletal regions within cells, as well as tissues, providing them with specific advantage for super-resolution imaging, as they place the fluorophore with a few nanometres of the target protein being imaged. We expect these novel tools to be of broad interest to many cell biologists and anticipate them becoming the tools of choice for super-resolution imaging.
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spelling pubmed-94508892022-10-25 Affimers and nanobodies as molecular probes and their applications in imaging Cordell, Paul Carrington, Glenn Curd, Alistair Parker, Francine Tomlinson, Darren Peckham, Michelle J Cell Sci Review Antibodies are the most widely used, traditional tool for labelling molecules in cells. In the past five to ten years, many new labelling tools have been developed with significant advantages over the traditional antibody. Here, we focus on nanobodies and the non-antibody binding scaffold proteins called Affimers. We explain how they are generated, selected and produced, and we describe how their small size, high binding affinity and specificity provides them with many advantages compared to antibodies. Of particular importance, their small size enables them to better penetrate dense cytoskeletal regions within cells, as well as tissues, providing them with specific advantage for super-resolution imaging, as they place the fluorophore with a few nanometres of the target protein being imaged. We expect these novel tools to be of broad interest to many cell biologists and anticipate them becoming the tools of choice for super-resolution imaging. The Company of Biologists Ltd 2022-07-18 /pmc/articles/PMC9450889/ /pubmed/35848463 http://dx.doi.org/10.1242/jcs.259168 Text en © 2022. Published by The Company of Biologists Ltd https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Review
Cordell, Paul
Carrington, Glenn
Curd, Alistair
Parker, Francine
Tomlinson, Darren
Peckham, Michelle
Affimers and nanobodies as molecular probes and their applications in imaging
title Affimers and nanobodies as molecular probes and their applications in imaging
title_full Affimers and nanobodies as molecular probes and their applications in imaging
title_fullStr Affimers and nanobodies as molecular probes and their applications in imaging
title_full_unstemmed Affimers and nanobodies as molecular probes and their applications in imaging
title_short Affimers and nanobodies as molecular probes and their applications in imaging
title_sort affimers and nanobodies as molecular probes and their applications in imaging
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9450889/
https://www.ncbi.nlm.nih.gov/pubmed/35848463
http://dx.doi.org/10.1242/jcs.259168
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