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Evaluation of minimally invasive sampling methods for detecting Avipoxvirus: Hummingbirds as a case example

Avian pox is a common avian virus that in its cutaneous form can cause characteristic lesions on a bird's dermal surfaces. Detection of avian pox in free-ranging birds historically relied on observations of visual lesions and/or histopathology, both which can underestimate avian pox prevalence....

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Autores principales: Galvin, Aoife N., Pandit, Pranav S., English, Simon G., Quock, Rachel C., Bandivadekar, Ruta R., Colwell, Rita R., Robinson, Barbara W., Ernest, Holly B., Brown, Mollie H., Sehgal, Ravinder N. M., Tell, Lisa A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9450938/
https://www.ncbi.nlm.nih.gov/pubmed/36090172
http://dx.doi.org/10.3389/fvets.2022.924854
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author Galvin, Aoife N.
Pandit, Pranav S.
English, Simon G.
Quock, Rachel C.
Bandivadekar, Ruta R.
Colwell, Rita R.
Robinson, Barbara W.
Ernest, Holly B.
Brown, Mollie H.
Sehgal, Ravinder N. M.
Tell, Lisa A.
author_facet Galvin, Aoife N.
Pandit, Pranav S.
English, Simon G.
Quock, Rachel C.
Bandivadekar, Ruta R.
Colwell, Rita R.
Robinson, Barbara W.
Ernest, Holly B.
Brown, Mollie H.
Sehgal, Ravinder N. M.
Tell, Lisa A.
author_sort Galvin, Aoife N.
collection PubMed
description Avian pox is a common avian virus that in its cutaneous form can cause characteristic lesions on a bird's dermal surfaces. Detection of avian pox in free-ranging birds historically relied on observations of visual lesions and/or histopathology, both which can underestimate avian pox prevalence. We compared traditional visual observation methods for avian pox with molecular methods that utilize minimally invasive samples (blood, toenail clipping, feathers, and dermal swabs) in an ecologically important group of birds, hummingbirds. Specifically, avian pox prevalence in several species of hummingbirds were examined across multiple locations using three different methods: (1) visual inspection of hummingbirds for pox-like lesions from a long-term banding data set, (2) qPCR assay of samples from hummingbird carcasses from wildlife rehabilitation centers, and (3) qPCR assay of samples from live-caught hummingbirds. A stark difference in prevalences among these three methods was identified, with an avian pox prevalence of 1.5% from banding data, 20.4% from hummingbird carcasses, and 32.5% from live-caught hummingbirds in California. This difference in detection rates underlines the necessity of a molecular method to survey for avian pox, and this study establishes one such method that could be applied to other wild bird species. Across all three methods, Anna's hummingbirds harbored significantly higher avian pox prevalence than other species examined, as did males compared with females and birds caught in Southern California compared with Northern California. After hatch-year hummingbirds also harbored higher avian pox prevalences than hatch-year hummingbirds in the California banding data set and the carcass data set. This is the first study to estimate the prevalence of avian pox in hummingbirds and address the ecology of this hummingbird-specific strain of avian pox virus, providing vital information to inform future studies on this charismatic and ecologically important group of birds.
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spelling pubmed-94509382022-09-08 Evaluation of minimally invasive sampling methods for detecting Avipoxvirus: Hummingbirds as a case example Galvin, Aoife N. Pandit, Pranav S. English, Simon G. Quock, Rachel C. Bandivadekar, Ruta R. Colwell, Rita R. Robinson, Barbara W. Ernest, Holly B. Brown, Mollie H. Sehgal, Ravinder N. M. Tell, Lisa A. Front Vet Sci Veterinary Science Avian pox is a common avian virus that in its cutaneous form can cause characteristic lesions on a bird's dermal surfaces. Detection of avian pox in free-ranging birds historically relied on observations of visual lesions and/or histopathology, both which can underestimate avian pox prevalence. We compared traditional visual observation methods for avian pox with molecular methods that utilize minimally invasive samples (blood, toenail clipping, feathers, and dermal swabs) in an ecologically important group of birds, hummingbirds. Specifically, avian pox prevalence in several species of hummingbirds were examined across multiple locations using three different methods: (1) visual inspection of hummingbirds for pox-like lesions from a long-term banding data set, (2) qPCR assay of samples from hummingbird carcasses from wildlife rehabilitation centers, and (3) qPCR assay of samples from live-caught hummingbirds. A stark difference in prevalences among these three methods was identified, with an avian pox prevalence of 1.5% from banding data, 20.4% from hummingbird carcasses, and 32.5% from live-caught hummingbirds in California. This difference in detection rates underlines the necessity of a molecular method to survey for avian pox, and this study establishes one such method that could be applied to other wild bird species. Across all three methods, Anna's hummingbirds harbored significantly higher avian pox prevalence than other species examined, as did males compared with females and birds caught in Southern California compared with Northern California. After hatch-year hummingbirds also harbored higher avian pox prevalences than hatch-year hummingbirds in the California banding data set and the carcass data set. This is the first study to estimate the prevalence of avian pox in hummingbirds and address the ecology of this hummingbird-specific strain of avian pox virus, providing vital information to inform future studies on this charismatic and ecologically important group of birds. Frontiers Media S.A. 2022-08-24 /pmc/articles/PMC9450938/ /pubmed/36090172 http://dx.doi.org/10.3389/fvets.2022.924854 Text en Copyright © 2022 Galvin, Pandit, English, Quock, Bandivadekar, Colwell, Robinson, Ernest, Brown, Sehgal and Tell. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Galvin, Aoife N.
Pandit, Pranav S.
English, Simon G.
Quock, Rachel C.
Bandivadekar, Ruta R.
Colwell, Rita R.
Robinson, Barbara W.
Ernest, Holly B.
Brown, Mollie H.
Sehgal, Ravinder N. M.
Tell, Lisa A.
Evaluation of minimally invasive sampling methods for detecting Avipoxvirus: Hummingbirds as a case example
title Evaluation of minimally invasive sampling methods for detecting Avipoxvirus: Hummingbirds as a case example
title_full Evaluation of minimally invasive sampling methods for detecting Avipoxvirus: Hummingbirds as a case example
title_fullStr Evaluation of minimally invasive sampling methods for detecting Avipoxvirus: Hummingbirds as a case example
title_full_unstemmed Evaluation of minimally invasive sampling methods for detecting Avipoxvirus: Hummingbirds as a case example
title_short Evaluation of minimally invasive sampling methods for detecting Avipoxvirus: Hummingbirds as a case example
title_sort evaluation of minimally invasive sampling methods for detecting avipoxvirus: hummingbirds as a case example
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9450938/
https://www.ncbi.nlm.nih.gov/pubmed/36090172
http://dx.doi.org/10.3389/fvets.2022.924854
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