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Asymmetric depth‐filtration: A versatile and scalable method for high‐yield isolation of extracellular vesicles with low contamination

We developed a novel asymmetric depth filtration (DF) approach to isolate extracellular vesicles (EVs) from biological fluids that outperforms ultracentrifugation and size‐exclusion chromatography in purity and yield of isolated EVs. By these metrics, a single‐step DF matches or exceeds the performa...

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Detalles Bibliográficos
Autores principales: Chernyshev, Vasiliy S., Chuprov‐Netochin, Roman N., Tsydenzhapova, Ekaterina, Svirshchevskaya, Elena V., Poltavtseva, Rimma A., Merdalimova, Anastasiia, Yashchenok, Alexey, Keshelava, Amiran, Sorokin, Konstantin, Keshelava, Varlam, Sukhikh, Gennadiy T., Gorin, Dmitry, Leonov, Sergey, Skliar, Mikhail
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9451526/
https://www.ncbi.nlm.nih.gov/pubmed/35942823
http://dx.doi.org/10.1002/jev2.12256