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Tridax procumbens Ameliorates Streptozotocin-Induced Diabetic Neuropathy in Rats via Modulating Angiogenic, Inflammatory, and Oxidative Pathways

Tridax procumbens (TP) is a traditional Indian therapeutic plant and was evaluated for its blood glucose lowering abilities, as well as for its ability to curb diabetic neuropathy (DN). Administrating 45 mg/kg body weight of streptozotocin (STZ) intraperitoneally for four weeks, DN was induced in Wi...

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Autores principales: Kakkar, Munish, Behl, Tapan, Cruz, Celia Vargas-De-La, Makeen, Hafiz A., Albratty, Mohammed, Alhazmi, Hassan A., Meraya, Abdulkarim M., Albadrani, Ghadeer M., Abdel-Daim, Mohamed M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9451972/
https://www.ncbi.nlm.nih.gov/pubmed/36091594
http://dx.doi.org/10.1155/2022/1795405
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author Kakkar, Munish
Behl, Tapan
Cruz, Celia Vargas-De-La
Makeen, Hafiz A.
Albratty, Mohammed
Alhazmi, Hassan A.
Meraya, Abdulkarim M.
Albadrani, Ghadeer M.
Abdel-Daim, Mohamed M.
author_facet Kakkar, Munish
Behl, Tapan
Cruz, Celia Vargas-De-La
Makeen, Hafiz A.
Albratty, Mohammed
Alhazmi, Hassan A.
Meraya, Abdulkarim M.
Albadrani, Ghadeer M.
Abdel-Daim, Mohamed M.
author_sort Kakkar, Munish
collection PubMed
description Tridax procumbens (TP) is a traditional Indian therapeutic plant and was evaluated for its blood glucose lowering abilities, as well as for its ability to curb diabetic neuropathy (DN). Administrating 45 mg/kg body weight of streptozotocin (STZ) intraperitoneally for four weeks, DN was induced in Wistar rats. After the rats' tails were clipped, the blood glucose levels were measured. Body weight and urine volume were also assessed. Oxidative stress makers such as superoxide dismutase (SOD), thiobarbituric acid reactive substances (TBARS), catalase (CAT), inflammatory cytokines for instance tumor necrosis factor (TNF)-α, and interleukin (IL)-1β were estimated. Further, protein kinase C (PKC-β) and vascular endothelial growth factor (VEGF) were also estimated as angiogenic markers. Behavioral parameters were also evaluated by using cold allodynia using acetone test, hot allodynia using Eddy's hot plate, grip strength test using Rota rod, and hyperalgesia test using Tail flick technique. The statistical assessment of findings was done employing one-way (ANOVA) analysis of variance, and subsequently Turkey as post hoc with GraphPad Prism software package. The ingestion of TP for 1 month in DN rats stemmed in a substantial decline in blood glucose concentrations matched to nontreated rats with DN. There had been a considerable improvement in DN as evident from the finding from biochemical markers. The serum level of antioxidant defense enzymes was significantly increased, while the activities of TBARS had been substantially reduced in the TP treated rats with DN. TP averted DN-triggered surge levels of TNF-α and IL-6 in the serum. Further, PKC-β and VEGF concentrations had been also reduced by the treatment TP. The findings of this research demonstrated that the restorative impact of TP on DN rats might be linked to the anti-inflammatory and antioxidative antiangiogenic retorts.
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spelling pubmed-94519722022-09-08 Tridax procumbens Ameliorates Streptozotocin-Induced Diabetic Neuropathy in Rats via Modulating Angiogenic, Inflammatory, and Oxidative Pathways Kakkar, Munish Behl, Tapan Cruz, Celia Vargas-De-La Makeen, Hafiz A. Albratty, Mohammed Alhazmi, Hassan A. Meraya, Abdulkarim M. Albadrani, Ghadeer M. Abdel-Daim, Mohamed M. Evid Based Complement Alternat Med Research Article Tridax procumbens (TP) is a traditional Indian therapeutic plant and was evaluated for its blood glucose lowering abilities, as well as for its ability to curb diabetic neuropathy (DN). Administrating 45 mg/kg body weight of streptozotocin (STZ) intraperitoneally for four weeks, DN was induced in Wistar rats. After the rats' tails were clipped, the blood glucose levels were measured. Body weight and urine volume were also assessed. Oxidative stress makers such as superoxide dismutase (SOD), thiobarbituric acid reactive substances (TBARS), catalase (CAT), inflammatory cytokines for instance tumor necrosis factor (TNF)-α, and interleukin (IL)-1β were estimated. Further, protein kinase C (PKC-β) and vascular endothelial growth factor (VEGF) were also estimated as angiogenic markers. Behavioral parameters were also evaluated by using cold allodynia using acetone test, hot allodynia using Eddy's hot plate, grip strength test using Rota rod, and hyperalgesia test using Tail flick technique. The statistical assessment of findings was done employing one-way (ANOVA) analysis of variance, and subsequently Turkey as post hoc with GraphPad Prism software package. The ingestion of TP for 1 month in DN rats stemmed in a substantial decline in blood glucose concentrations matched to nontreated rats with DN. There had been a considerable improvement in DN as evident from the finding from biochemical markers. The serum level of antioxidant defense enzymes was significantly increased, while the activities of TBARS had been substantially reduced in the TP treated rats with DN. TP averted DN-triggered surge levels of TNF-α and IL-6 in the serum. Further, PKC-β and VEGF concentrations had been also reduced by the treatment TP. The findings of this research demonstrated that the restorative impact of TP on DN rats might be linked to the anti-inflammatory and antioxidative antiangiogenic retorts. Hindawi 2022-08-31 /pmc/articles/PMC9451972/ /pubmed/36091594 http://dx.doi.org/10.1155/2022/1795405 Text en Copyright © 2022 Munish Kakkar et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kakkar, Munish
Behl, Tapan
Cruz, Celia Vargas-De-La
Makeen, Hafiz A.
Albratty, Mohammed
Alhazmi, Hassan A.
Meraya, Abdulkarim M.
Albadrani, Ghadeer M.
Abdel-Daim, Mohamed M.
Tridax procumbens Ameliorates Streptozotocin-Induced Diabetic Neuropathy in Rats via Modulating Angiogenic, Inflammatory, and Oxidative Pathways
title Tridax procumbens Ameliorates Streptozotocin-Induced Diabetic Neuropathy in Rats via Modulating Angiogenic, Inflammatory, and Oxidative Pathways
title_full Tridax procumbens Ameliorates Streptozotocin-Induced Diabetic Neuropathy in Rats via Modulating Angiogenic, Inflammatory, and Oxidative Pathways
title_fullStr Tridax procumbens Ameliorates Streptozotocin-Induced Diabetic Neuropathy in Rats via Modulating Angiogenic, Inflammatory, and Oxidative Pathways
title_full_unstemmed Tridax procumbens Ameliorates Streptozotocin-Induced Diabetic Neuropathy in Rats via Modulating Angiogenic, Inflammatory, and Oxidative Pathways
title_short Tridax procumbens Ameliorates Streptozotocin-Induced Diabetic Neuropathy in Rats via Modulating Angiogenic, Inflammatory, and Oxidative Pathways
title_sort tridax procumbens ameliorates streptozotocin-induced diabetic neuropathy in rats via modulating angiogenic, inflammatory, and oxidative pathways
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9451972/
https://www.ncbi.nlm.nih.gov/pubmed/36091594
http://dx.doi.org/10.1155/2022/1795405
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