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Monitoring of SARS-CoV-2 circulation using saliva testing in school children in Rome, Italy

OBJECTIVES: To describe the trend of SARS-CoV-2 RNA in saliva samples from children attending nine schools in Rome in the local surveillance unit RM3 during the period of September 2021-March 2022, in parallel with the trend of SARS-CoV-2 RNA observed in nasopharyngeal swabs (NPSs) from the populati...

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Autores principales: Sberna, Giuseppe, Guarini, Rosanna, Vaia, Francesco, Maggi, Fabrizio, Bordi, Licia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Authors. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9452395/
https://www.ncbi.nlm.nih.gov/pubmed/36089150
http://dx.doi.org/10.1016/j.ijid.2022.09.007
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author Sberna, Giuseppe
Guarini, Rosanna
Vaia, Francesco
Maggi, Fabrizio
Bordi, Licia
author_facet Sberna, Giuseppe
Guarini, Rosanna
Vaia, Francesco
Maggi, Fabrizio
Bordi, Licia
author_sort Sberna, Giuseppe
collection PubMed
description OBJECTIVES: To describe the trend of SARS-CoV-2 RNA in saliva samples from children attending nine schools in Rome in the local surveillance unit RM3 during the period of September 2021-March 2022, in parallel with the trend of SARS-CoV-2 RNA observed in nasopharyngeal swabs (NPSs) from the population in the same catchment area that was routinely tested at our laboratory in the same period. METHODS: Saliva samples were collected using the Copan LolliSponge(TM) device and analyzed by Aptima® SARS-CoV-2 Assay on the Panther® System. NPSs were tested using either Aptima® SARS-CoV-2 Assay or Alinity m SARS-CoV-2 Assay. RESULTS: The percentage of positivity in the two populations was different; of the 2222 saliva samples from students, 0.99% had positive results, whereas the percentage was higher (33.43%) in the 8994 NPSs representing the population from local surveillance unit RM3. Interestingly, the trend of SARS-CoV-2 RNA in saliva samples from students was consistent with that observed in NPSs from the population in same catchment area, although with peaks slightly anticipated. CONCLUSION: Overall, screening of saliva in the schools represents a good system to monitor SARS-CoV-2 circulation in the population, allowing early detection and quick isolation of students who are asymptomatic with positive test results and thus prevention of virus transmission.
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spelling pubmed-94523952022-09-08 Monitoring of SARS-CoV-2 circulation using saliva testing in school children in Rome, Italy Sberna, Giuseppe Guarini, Rosanna Vaia, Francesco Maggi, Fabrizio Bordi, Licia Int J Infect Dis Short Communication OBJECTIVES: To describe the trend of SARS-CoV-2 RNA in saliva samples from children attending nine schools in Rome in the local surveillance unit RM3 during the period of September 2021-March 2022, in parallel with the trend of SARS-CoV-2 RNA observed in nasopharyngeal swabs (NPSs) from the population in the same catchment area that was routinely tested at our laboratory in the same period. METHODS: Saliva samples were collected using the Copan LolliSponge(TM) device and analyzed by Aptima® SARS-CoV-2 Assay on the Panther® System. NPSs were tested using either Aptima® SARS-CoV-2 Assay or Alinity m SARS-CoV-2 Assay. RESULTS: The percentage of positivity in the two populations was different; of the 2222 saliva samples from students, 0.99% had positive results, whereas the percentage was higher (33.43%) in the 8994 NPSs representing the population from local surveillance unit RM3. Interestingly, the trend of SARS-CoV-2 RNA in saliva samples from students was consistent with that observed in NPSs from the population in same catchment area, although with peaks slightly anticipated. CONCLUSION: Overall, screening of saliva in the schools represents a good system to monitor SARS-CoV-2 circulation in the population, allowing early detection and quick isolation of students who are asymptomatic with positive test results and thus prevention of virus transmission. The Authors. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. 2022-11 2022-09-08 /pmc/articles/PMC9452395/ /pubmed/36089150 http://dx.doi.org/10.1016/j.ijid.2022.09.007 Text en © 2022 The Authors Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Short Communication
Sberna, Giuseppe
Guarini, Rosanna
Vaia, Francesco
Maggi, Fabrizio
Bordi, Licia
Monitoring of SARS-CoV-2 circulation using saliva testing in school children in Rome, Italy
title Monitoring of SARS-CoV-2 circulation using saliva testing in school children in Rome, Italy
title_full Monitoring of SARS-CoV-2 circulation using saliva testing in school children in Rome, Italy
title_fullStr Monitoring of SARS-CoV-2 circulation using saliva testing in school children in Rome, Italy
title_full_unstemmed Monitoring of SARS-CoV-2 circulation using saliva testing in school children in Rome, Italy
title_short Monitoring of SARS-CoV-2 circulation using saliva testing in school children in Rome, Italy
title_sort monitoring of sars-cov-2 circulation using saliva testing in school children in rome, italy
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9452395/
https://www.ncbi.nlm.nih.gov/pubmed/36089150
http://dx.doi.org/10.1016/j.ijid.2022.09.007
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