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The integration host factor regulates multiple virulence pathways in bacterial pathogen Dickeya zeae MS2
Dickeya zeae is an aggressive bacterial phytopathogen that infects a wide range of host plants. It has been reported that integration host factor (IHF), a nucleoid‐associated protein consisting of IHFα and IHFβ subunits, regulates gene expression by influencing nucleoid structure and DNA bending. To...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9452768/ https://www.ncbi.nlm.nih.gov/pubmed/35819797 http://dx.doi.org/10.1111/mpp.13244 |
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author | Chen, Shanshan Hu, Ming Hu, Anqun Xue, Yang Wang, Si Liu, Fan Li, Chuhao Zhou, Xiaofan Zhou, Jianuan |
author_facet | Chen, Shanshan Hu, Ming Hu, Anqun Xue, Yang Wang, Si Liu, Fan Li, Chuhao Zhou, Xiaofan Zhou, Jianuan |
author_sort | Chen, Shanshan |
collection | PubMed |
description | Dickeya zeae is an aggressive bacterial phytopathogen that infects a wide range of host plants. It has been reported that integration host factor (IHF), a nucleoid‐associated protein consisting of IHFα and IHFβ subunits, regulates gene expression by influencing nucleoid structure and DNA bending. To define the role of IHF in the pathogenesis of D. zeae MS2, we deleted either and both of the IHF subunit encoding genes ihfA and ihfB, which significantly reduced the production of cell wall‐degrading enzymes (CWDEs), an unknown novel phytotoxin and the virulence factor‐modulating (VFM) quorum‐sensing (QS) signal, cell motility, biofilm formation, and thereafter the infection ability towards both potato slices and banana seedlings. To characterize the regulatory pathways of IHF protein associated with virulence, IHF binding sites (consensus sequence 5′‐WATCAANNNNTTR‐3′) were predicted and 272 binding sites were found throughout the genome. The expression of 110 tested genes was affected by IHF. Electrophoretic mobility shift assay (EMSA) showed direct interaction of IhfA protein with the promoters of vfmE, speA, pipR, fis, slyA, prtD, hrpL, hecB, hcp, indA, hdaA, flhD, pilT, gcpJ, arcA, arcB, and lysR. This study clarified the contribution of IHF in the pathogenic process of D. zeae by controlling the production of VFM and putrescine QS signals, phytotoxin, and indigoidine, the luxR‐solo system, Fis, SlyA, and FlhD transcriptional regulators, and secretion systems from type I to type VI. Characterization of the regulatory networks of IHF in D. zeae provides a target for prevention and control of plant soft rot disease. |
format | Online Article Text |
id | pubmed-9452768 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94527682022-09-10 The integration host factor regulates multiple virulence pathways in bacterial pathogen Dickeya zeae MS2 Chen, Shanshan Hu, Ming Hu, Anqun Xue, Yang Wang, Si Liu, Fan Li, Chuhao Zhou, Xiaofan Zhou, Jianuan Mol Plant Pathol Original Articles Dickeya zeae is an aggressive bacterial phytopathogen that infects a wide range of host plants. It has been reported that integration host factor (IHF), a nucleoid‐associated protein consisting of IHFα and IHFβ subunits, regulates gene expression by influencing nucleoid structure and DNA bending. To define the role of IHF in the pathogenesis of D. zeae MS2, we deleted either and both of the IHF subunit encoding genes ihfA and ihfB, which significantly reduced the production of cell wall‐degrading enzymes (CWDEs), an unknown novel phytotoxin and the virulence factor‐modulating (VFM) quorum‐sensing (QS) signal, cell motility, biofilm formation, and thereafter the infection ability towards both potato slices and banana seedlings. To characterize the regulatory pathways of IHF protein associated with virulence, IHF binding sites (consensus sequence 5′‐WATCAANNNNTTR‐3′) were predicted and 272 binding sites were found throughout the genome. The expression of 110 tested genes was affected by IHF. Electrophoretic mobility shift assay (EMSA) showed direct interaction of IhfA protein with the promoters of vfmE, speA, pipR, fis, slyA, prtD, hrpL, hecB, hcp, indA, hdaA, flhD, pilT, gcpJ, arcA, arcB, and lysR. This study clarified the contribution of IHF in the pathogenic process of D. zeae by controlling the production of VFM and putrescine QS signals, phytotoxin, and indigoidine, the luxR‐solo system, Fis, SlyA, and FlhD transcriptional regulators, and secretion systems from type I to type VI. Characterization of the regulatory networks of IHF in D. zeae provides a target for prevention and control of plant soft rot disease. John Wiley and Sons Inc. 2022-07-12 /pmc/articles/PMC9452768/ /pubmed/35819797 http://dx.doi.org/10.1111/mpp.13244 Text en © 2022 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Original Articles Chen, Shanshan Hu, Ming Hu, Anqun Xue, Yang Wang, Si Liu, Fan Li, Chuhao Zhou, Xiaofan Zhou, Jianuan The integration host factor regulates multiple virulence pathways in bacterial pathogen Dickeya zeae MS2 |
title | The integration host factor regulates multiple virulence pathways in bacterial pathogen Dickeya zeae MS2 |
title_full | The integration host factor regulates multiple virulence pathways in bacterial pathogen Dickeya zeae MS2 |
title_fullStr | The integration host factor regulates multiple virulence pathways in bacterial pathogen Dickeya zeae MS2 |
title_full_unstemmed | The integration host factor regulates multiple virulence pathways in bacterial pathogen Dickeya zeae MS2 |
title_short | The integration host factor regulates multiple virulence pathways in bacterial pathogen Dickeya zeae MS2 |
title_sort | integration host factor regulates multiple virulence pathways in bacterial pathogen dickeya zeae ms2 |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9452768/ https://www.ncbi.nlm.nih.gov/pubmed/35819797 http://dx.doi.org/10.1111/mpp.13244 |
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