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Sulforaphane, a secondary metabolite in crucifers, inhibits the oxidative stress adaptation and virulence of Xanthomonas by directly targeting OxyR
Plant secondary metabolites perform numerous functions in the interactions between plants and pathogens. However, little is known about the precise mechanisms underlying their contribution to the direct inhibition of pathogen growth and virulence in planta. Here, we show that the secondary metabolit...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9452769/ https://www.ncbi.nlm.nih.gov/pubmed/35942507 http://dx.doi.org/10.1111/mpp.13245 |
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author | Wang, Bo Li, Kaihuai Wu, Guichun Xu, Zhizhou Hou, Rongxian Guo, Baodian Zhao, Yancun Liu, Fengquan |
author_facet | Wang, Bo Li, Kaihuai Wu, Guichun Xu, Zhizhou Hou, Rongxian Guo, Baodian Zhao, Yancun Liu, Fengquan |
author_sort | Wang, Bo |
collection | PubMed |
description | Plant secondary metabolites perform numerous functions in the interactions between plants and pathogens. However, little is known about the precise mechanisms underlying their contribution to the direct inhibition of pathogen growth and virulence in planta. Here, we show that the secondary metabolite sulforaphane (SFN) in crucifers inhibits the growth, virulence, and ability of Xanthomonas species to adapt to oxidative stress, which is essential for the successful infection of host plants by phytopathogens. The transcription of oxidative stress detoxification‐related genes (catalase [katA and katG] and alkylhydroperoxide‐NADPH oxidoreductase subunit C [ahpC]) was substantially inhibited by SFN in Xanthomonas campestris pv. campestris (Xcc), and this phenomenon was most obvious in sax gene mutants sensitive to SFN. By performing microscale thermophoresis (MST) and electrophoretic mobility shift assay (EMSA), we observed that SFN directly bound to the virulence‐related redox‐sensing transcription factor OxyR and weakened the ability of OxyR to bind to the promoters of oxidative stress detoxification‐related genes. Collectively, these results illustrate that SFN directly targets OxyR to inhibit the bacterial adaptation to oxidative stress, thereby decreasing bacterial virulence. Interestingly, this phenomenon occurs in multiple Xanthomonas species. This study provides novel insights into the molecular mechanisms by which SFN limits Xanthomonas adaptation to oxidative stress and virulence, and the findings will facilitate future studies on the use of SFN as a biopesticide to control Xanthomonas. |
format | Online Article Text |
id | pubmed-9452769 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94527692022-09-10 Sulforaphane, a secondary metabolite in crucifers, inhibits the oxidative stress adaptation and virulence of Xanthomonas by directly targeting OxyR Wang, Bo Li, Kaihuai Wu, Guichun Xu, Zhizhou Hou, Rongxian Guo, Baodian Zhao, Yancun Liu, Fengquan Mol Plant Pathol Original Articles Plant secondary metabolites perform numerous functions in the interactions between plants and pathogens. However, little is known about the precise mechanisms underlying their contribution to the direct inhibition of pathogen growth and virulence in planta. Here, we show that the secondary metabolite sulforaphane (SFN) in crucifers inhibits the growth, virulence, and ability of Xanthomonas species to adapt to oxidative stress, which is essential for the successful infection of host plants by phytopathogens. The transcription of oxidative stress detoxification‐related genes (catalase [katA and katG] and alkylhydroperoxide‐NADPH oxidoreductase subunit C [ahpC]) was substantially inhibited by SFN in Xanthomonas campestris pv. campestris (Xcc), and this phenomenon was most obvious in sax gene mutants sensitive to SFN. By performing microscale thermophoresis (MST) and electrophoretic mobility shift assay (EMSA), we observed that SFN directly bound to the virulence‐related redox‐sensing transcription factor OxyR and weakened the ability of OxyR to bind to the promoters of oxidative stress detoxification‐related genes. Collectively, these results illustrate that SFN directly targets OxyR to inhibit the bacterial adaptation to oxidative stress, thereby decreasing bacterial virulence. Interestingly, this phenomenon occurs in multiple Xanthomonas species. This study provides novel insights into the molecular mechanisms by which SFN limits Xanthomonas adaptation to oxidative stress and virulence, and the findings will facilitate future studies on the use of SFN as a biopesticide to control Xanthomonas. John Wiley and Sons Inc. 2022-08-08 /pmc/articles/PMC9452769/ /pubmed/35942507 http://dx.doi.org/10.1111/mpp.13245 Text en © 2022 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Original Articles Wang, Bo Li, Kaihuai Wu, Guichun Xu, Zhizhou Hou, Rongxian Guo, Baodian Zhao, Yancun Liu, Fengquan Sulforaphane, a secondary metabolite in crucifers, inhibits the oxidative stress adaptation and virulence of Xanthomonas by directly targeting OxyR |
title | Sulforaphane, a secondary metabolite in crucifers, inhibits the oxidative stress adaptation and virulence of Xanthomonas by directly targeting OxyR |
title_full | Sulforaphane, a secondary metabolite in crucifers, inhibits the oxidative stress adaptation and virulence of Xanthomonas by directly targeting OxyR |
title_fullStr | Sulforaphane, a secondary metabolite in crucifers, inhibits the oxidative stress adaptation and virulence of Xanthomonas by directly targeting OxyR |
title_full_unstemmed | Sulforaphane, a secondary metabolite in crucifers, inhibits the oxidative stress adaptation and virulence of Xanthomonas by directly targeting OxyR |
title_short | Sulforaphane, a secondary metabolite in crucifers, inhibits the oxidative stress adaptation and virulence of Xanthomonas by directly targeting OxyR |
title_sort | sulforaphane, a secondary metabolite in crucifers, inhibits the oxidative stress adaptation and virulence of xanthomonas by directly targeting oxyr |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9452769/ https://www.ncbi.nlm.nih.gov/pubmed/35942507 http://dx.doi.org/10.1111/mpp.13245 |
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