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Sulforaphane, a secondary metabolite in crucifers, inhibits the oxidative stress adaptation and virulence of Xanthomonas by directly targeting OxyR

Plant secondary metabolites perform numerous functions in the interactions between plants and pathogens. However, little is known about the precise mechanisms underlying their contribution to the direct inhibition of pathogen growth and virulence in planta. Here, we show that the secondary metabolit...

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Autores principales: Wang, Bo, Li, Kaihuai, Wu, Guichun, Xu, Zhizhou, Hou, Rongxian, Guo, Baodian, Zhao, Yancun, Liu, Fengquan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9452769/
https://www.ncbi.nlm.nih.gov/pubmed/35942507
http://dx.doi.org/10.1111/mpp.13245
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author Wang, Bo
Li, Kaihuai
Wu, Guichun
Xu, Zhizhou
Hou, Rongxian
Guo, Baodian
Zhao, Yancun
Liu, Fengquan
author_facet Wang, Bo
Li, Kaihuai
Wu, Guichun
Xu, Zhizhou
Hou, Rongxian
Guo, Baodian
Zhao, Yancun
Liu, Fengquan
author_sort Wang, Bo
collection PubMed
description Plant secondary metabolites perform numerous functions in the interactions between plants and pathogens. However, little is known about the precise mechanisms underlying their contribution to the direct inhibition of pathogen growth and virulence in planta. Here, we show that the secondary metabolite sulforaphane (SFN) in crucifers inhibits the growth, virulence, and ability of Xanthomonas species to adapt to oxidative stress, which is essential for the successful infection of host plants by phytopathogens. The transcription of oxidative stress detoxification‐related genes (catalase [katA and katG] and alkylhydroperoxide‐NADPH oxidoreductase subunit C [ahpC]) was substantially inhibited by SFN in Xanthomonas campestris pv. campestris (Xcc), and this phenomenon was most obvious in sax gene mutants sensitive to SFN. By performing microscale thermophoresis (MST) and electrophoretic mobility shift assay (EMSA), we observed that SFN directly bound to the virulence‐related redox‐sensing transcription factor OxyR and weakened the ability of OxyR to bind to the promoters of oxidative stress detoxification‐related genes. Collectively, these results illustrate that SFN directly targets OxyR to inhibit the bacterial adaptation to oxidative stress, thereby decreasing bacterial virulence. Interestingly, this phenomenon occurs in multiple Xanthomonas species. This study provides novel insights into the molecular mechanisms by which SFN limits Xanthomonas adaptation to oxidative stress and virulence, and the findings will facilitate future studies on the use of SFN as a biopesticide to control Xanthomonas.
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spelling pubmed-94527692022-09-10 Sulforaphane, a secondary metabolite in crucifers, inhibits the oxidative stress adaptation and virulence of Xanthomonas by directly targeting OxyR Wang, Bo Li, Kaihuai Wu, Guichun Xu, Zhizhou Hou, Rongxian Guo, Baodian Zhao, Yancun Liu, Fengquan Mol Plant Pathol Original Articles Plant secondary metabolites perform numerous functions in the interactions between plants and pathogens. However, little is known about the precise mechanisms underlying their contribution to the direct inhibition of pathogen growth and virulence in planta. Here, we show that the secondary metabolite sulforaphane (SFN) in crucifers inhibits the growth, virulence, and ability of Xanthomonas species to adapt to oxidative stress, which is essential for the successful infection of host plants by phytopathogens. The transcription of oxidative stress detoxification‐related genes (catalase [katA and katG] and alkylhydroperoxide‐NADPH oxidoreductase subunit C [ahpC]) was substantially inhibited by SFN in Xanthomonas campestris pv. campestris (Xcc), and this phenomenon was most obvious in sax gene mutants sensitive to SFN. By performing microscale thermophoresis (MST) and electrophoretic mobility shift assay (EMSA), we observed that SFN directly bound to the virulence‐related redox‐sensing transcription factor OxyR and weakened the ability of OxyR to bind to the promoters of oxidative stress detoxification‐related genes. Collectively, these results illustrate that SFN directly targets OxyR to inhibit the bacterial adaptation to oxidative stress, thereby decreasing bacterial virulence. Interestingly, this phenomenon occurs in multiple Xanthomonas species. This study provides novel insights into the molecular mechanisms by which SFN limits Xanthomonas adaptation to oxidative stress and virulence, and the findings will facilitate future studies on the use of SFN as a biopesticide to control Xanthomonas. John Wiley and Sons Inc. 2022-08-08 /pmc/articles/PMC9452769/ /pubmed/35942507 http://dx.doi.org/10.1111/mpp.13245 Text en © 2022 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Wang, Bo
Li, Kaihuai
Wu, Guichun
Xu, Zhizhou
Hou, Rongxian
Guo, Baodian
Zhao, Yancun
Liu, Fengquan
Sulforaphane, a secondary metabolite in crucifers, inhibits the oxidative stress adaptation and virulence of Xanthomonas by directly targeting OxyR
title Sulforaphane, a secondary metabolite in crucifers, inhibits the oxidative stress adaptation and virulence of Xanthomonas by directly targeting OxyR
title_full Sulforaphane, a secondary metabolite in crucifers, inhibits the oxidative stress adaptation and virulence of Xanthomonas by directly targeting OxyR
title_fullStr Sulforaphane, a secondary metabolite in crucifers, inhibits the oxidative stress adaptation and virulence of Xanthomonas by directly targeting OxyR
title_full_unstemmed Sulforaphane, a secondary metabolite in crucifers, inhibits the oxidative stress adaptation and virulence of Xanthomonas by directly targeting OxyR
title_short Sulforaphane, a secondary metabolite in crucifers, inhibits the oxidative stress adaptation and virulence of Xanthomonas by directly targeting OxyR
title_sort sulforaphane, a secondary metabolite in crucifers, inhibits the oxidative stress adaptation and virulence of xanthomonas by directly targeting oxyr
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9452769/
https://www.ncbi.nlm.nih.gov/pubmed/35942507
http://dx.doi.org/10.1111/mpp.13245
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