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Palmitoylethanolamide attenuates neurodevelopmental delay and early hippocampal damage following perinatal asphyxia in rats

Impaired gas exchange close to labor causes perinatal asphyxia (PA), a neurodevelopmental impairment factor. Palmitoylethanolamide (PEA) proved neuroprotective in experimental brain injury and neurodegeneration models. This study aimed to evaluate PEA effects on the immature-brain, i.e., early neuro...

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Autores principales: Herrera, Maria I., Udovin, Lucas D., Kobiec, Tamara, Toro-Urrego, Nicolas, Kusnier, Carlos F., Kölliker-Frers, Rodolfo A., Luaces, Juan P., Otero-Losada, Matilde, Capani, Francisco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9452789/
https://www.ncbi.nlm.nih.gov/pubmed/36090655
http://dx.doi.org/10.3389/fnbeh.2022.953157
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author Herrera, Maria I.
Udovin, Lucas D.
Kobiec, Tamara
Toro-Urrego, Nicolas
Kusnier, Carlos F.
Kölliker-Frers, Rodolfo A.
Luaces, Juan P.
Otero-Losada, Matilde
Capani, Francisco
author_facet Herrera, Maria I.
Udovin, Lucas D.
Kobiec, Tamara
Toro-Urrego, Nicolas
Kusnier, Carlos F.
Kölliker-Frers, Rodolfo A.
Luaces, Juan P.
Otero-Losada, Matilde
Capani, Francisco
author_sort Herrera, Maria I.
collection PubMed
description Impaired gas exchange close to labor causes perinatal asphyxia (PA), a neurodevelopmental impairment factor. Palmitoylethanolamide (PEA) proved neuroprotective in experimental brain injury and neurodegeneration models. This study aimed to evaluate PEA effects on the immature-brain, i.e., early neuroprotection by PEA in an experimental PA paradigm. Newborn rats were placed in a 37°C water bath for 19 min to induce PA. PEA 10 mg/kg, s.c., was administered within the first hour of life. Neurobehavioral responses were assessed from postnatal day 1 (P1) to postnatal day 21 (P21), recording the day of appearance of several reflexes and neurological signs. Hippocampal CA1 area ultrastructure was examined using electron microscopy. Microtubule-associated protein 2 (MAP-2), phosphorylated high and medium molecular weight neurofilaments (pNF H/M), and glial fibrillary acidic protein (GFAP) were assessed using immunohistochemistry and Western blot at P21. Over the first 3 weeks of life, PA rats showed late gait, negative geotaxis and eye-opening onset, and delayed appearance of air-righting, auditory startle, sensory eyelid, forelimb placing, and grasp reflexes. On P21, the hippocampal CA1 area showed signs of neuronal degeneration and MAP-2 deficit. PEA treatment reduced PA-induced hippocampal damage and normalized the time of appearance of gait, air-righting, placing, and grasp reflexes. The outcome of this study might prove useful in designing intervention strategies to reduce early neurodevelopmental delay following PA.
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spelling pubmed-94527892022-09-09 Palmitoylethanolamide attenuates neurodevelopmental delay and early hippocampal damage following perinatal asphyxia in rats Herrera, Maria I. Udovin, Lucas D. Kobiec, Tamara Toro-Urrego, Nicolas Kusnier, Carlos F. Kölliker-Frers, Rodolfo A. Luaces, Juan P. Otero-Losada, Matilde Capani, Francisco Front Behav Neurosci Neuroscience Impaired gas exchange close to labor causes perinatal asphyxia (PA), a neurodevelopmental impairment factor. Palmitoylethanolamide (PEA) proved neuroprotective in experimental brain injury and neurodegeneration models. This study aimed to evaluate PEA effects on the immature-brain, i.e., early neuroprotection by PEA in an experimental PA paradigm. Newborn rats were placed in a 37°C water bath for 19 min to induce PA. PEA 10 mg/kg, s.c., was administered within the first hour of life. Neurobehavioral responses were assessed from postnatal day 1 (P1) to postnatal day 21 (P21), recording the day of appearance of several reflexes and neurological signs. Hippocampal CA1 area ultrastructure was examined using electron microscopy. Microtubule-associated protein 2 (MAP-2), phosphorylated high and medium molecular weight neurofilaments (pNF H/M), and glial fibrillary acidic protein (GFAP) were assessed using immunohistochemistry and Western blot at P21. Over the first 3 weeks of life, PA rats showed late gait, negative geotaxis and eye-opening onset, and delayed appearance of air-righting, auditory startle, sensory eyelid, forelimb placing, and grasp reflexes. On P21, the hippocampal CA1 area showed signs of neuronal degeneration and MAP-2 deficit. PEA treatment reduced PA-induced hippocampal damage and normalized the time of appearance of gait, air-righting, placing, and grasp reflexes. The outcome of this study might prove useful in designing intervention strategies to reduce early neurodevelopmental delay following PA. Frontiers Media S.A. 2022-08-25 /pmc/articles/PMC9452789/ /pubmed/36090655 http://dx.doi.org/10.3389/fnbeh.2022.953157 Text en Copyright © 2022 Herrera, Udovin, Kobiec, Toro-Urrego, Kusnier, Kölliker-Frers, Luaces, Otero-Losada and Capani. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Herrera, Maria I.
Udovin, Lucas D.
Kobiec, Tamara
Toro-Urrego, Nicolas
Kusnier, Carlos F.
Kölliker-Frers, Rodolfo A.
Luaces, Juan P.
Otero-Losada, Matilde
Capani, Francisco
Palmitoylethanolamide attenuates neurodevelopmental delay and early hippocampal damage following perinatal asphyxia in rats
title Palmitoylethanolamide attenuates neurodevelopmental delay and early hippocampal damage following perinatal asphyxia in rats
title_full Palmitoylethanolamide attenuates neurodevelopmental delay and early hippocampal damage following perinatal asphyxia in rats
title_fullStr Palmitoylethanolamide attenuates neurodevelopmental delay and early hippocampal damage following perinatal asphyxia in rats
title_full_unstemmed Palmitoylethanolamide attenuates neurodevelopmental delay and early hippocampal damage following perinatal asphyxia in rats
title_short Palmitoylethanolamide attenuates neurodevelopmental delay and early hippocampal damage following perinatal asphyxia in rats
title_sort palmitoylethanolamide attenuates neurodevelopmental delay and early hippocampal damage following perinatal asphyxia in rats
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9452789/
https://www.ncbi.nlm.nih.gov/pubmed/36090655
http://dx.doi.org/10.3389/fnbeh.2022.953157
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