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The hepatic extramedullary hematopoiesis during experimental murine Schistosomiasis mansoni

Many years ago, our research group has demonstrated extramedullary hematopoiesis in the peripheral zone of murine hepatic schistosomal granulomas. In the present study, we revisit this phenomenon using new technical and conceptual approaches. Therefore, newborn mice were percutaneously infected by S...

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Autores principales: Francisco, Juliane Siqueira, Terra, Marcia Andrea Barge Loução, Klein, Gabriel Couto Thurler, Dias de Oliveira, Barbara Cristina Euzebio Pereira, Pelajo-Machado, Marcelo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9453041/
https://www.ncbi.nlm.nih.gov/pubmed/36091027
http://dx.doi.org/10.3389/fimmu.2022.955034
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author Francisco, Juliane Siqueira
Terra, Marcia Andrea Barge Loução
Klein, Gabriel Couto Thurler
Dias de Oliveira, Barbara Cristina Euzebio Pereira
Pelajo-Machado, Marcelo
author_facet Francisco, Juliane Siqueira
Terra, Marcia Andrea Barge Loução
Klein, Gabriel Couto Thurler
Dias de Oliveira, Barbara Cristina Euzebio Pereira
Pelajo-Machado, Marcelo
author_sort Francisco, Juliane Siqueira
collection PubMed
description Many years ago, our research group has demonstrated extramedullary hematopoiesis in the peripheral zone of murine hepatic schistosomal granulomas. In the present study, we revisit this phenomenon using new technical and conceptual approaches. Therefore, newborn mice were percutaneously infected by Schistosoma mansoni cercariae and euthanized between 35- and 60-days post infection. Liver samples were submitted to histopathology and immunohistochemical analyses. Cells under mitosis and/or expressing Ki67 demonstrated the proliferation of hematopoietic cells both around the parasite’s eggs trapped in the liver and around hepatic vessels. After 50 days post infection, proliferating cells at different levels on differentiation were located preferentially in the peripheral zone of the granulomas, around the vessels and inside the sinusoids. The presence of acidic and sulfated glycoconjugates, reticular fibers and the absence of fibronectin characterized the microenvironment for attraction and maintenance of hematopoiesis. Some neutrophils secreted MMP9 from the earliest points of infection, indicating degradation of the extracellular matrix in regions of histolysis and a possible chemoattraction of hematopoietic stem cells to the liver. Fall-3+ cells and Sca-1+ cells indicated that early hematopoietic progenitors could be mobilized to the liver. Groups of vWF+ megakaryocytes suggest chemoattraction of these cells and/or migration, proliferation, and differentiation of very immature progenitors to this organ. The increase of blood vessels and extramedullary hematopoiesis in this environment, where markers of immature hematopoietic and endothelial cells have been identified, points to the possibility of the presence of progenitors for endothelial and hematopoietic cells in the liver during the infection. There is also the possibility of concomitant migration of more differentiated hematopoietic progenitors, that proliferate and differentiate in the liver, and the occurrence of angiogenesis caused by inflammation or release of ovular antigens that stimulate the activation and proliferation of endothelial cells. Altogether, these data increase knowledge about a murine model that is of interest for investigating the pathology of the schistosomiasis and also the dynamics of hematopoiesis.
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spelling pubmed-94530412022-09-09 The hepatic extramedullary hematopoiesis during experimental murine Schistosomiasis mansoni Francisco, Juliane Siqueira Terra, Marcia Andrea Barge Loução Klein, Gabriel Couto Thurler Dias de Oliveira, Barbara Cristina Euzebio Pereira Pelajo-Machado, Marcelo Front Immunol Immunology Many years ago, our research group has demonstrated extramedullary hematopoiesis in the peripheral zone of murine hepatic schistosomal granulomas. In the present study, we revisit this phenomenon using new technical and conceptual approaches. Therefore, newborn mice were percutaneously infected by Schistosoma mansoni cercariae and euthanized between 35- and 60-days post infection. Liver samples were submitted to histopathology and immunohistochemical analyses. Cells under mitosis and/or expressing Ki67 demonstrated the proliferation of hematopoietic cells both around the parasite’s eggs trapped in the liver and around hepatic vessels. After 50 days post infection, proliferating cells at different levels on differentiation were located preferentially in the peripheral zone of the granulomas, around the vessels and inside the sinusoids. The presence of acidic and sulfated glycoconjugates, reticular fibers and the absence of fibronectin characterized the microenvironment for attraction and maintenance of hematopoiesis. Some neutrophils secreted MMP9 from the earliest points of infection, indicating degradation of the extracellular matrix in regions of histolysis and a possible chemoattraction of hematopoietic stem cells to the liver. Fall-3+ cells and Sca-1+ cells indicated that early hematopoietic progenitors could be mobilized to the liver. Groups of vWF+ megakaryocytes suggest chemoattraction of these cells and/or migration, proliferation, and differentiation of very immature progenitors to this organ. The increase of blood vessels and extramedullary hematopoiesis in this environment, where markers of immature hematopoietic and endothelial cells have been identified, points to the possibility of the presence of progenitors for endothelial and hematopoietic cells in the liver during the infection. There is also the possibility of concomitant migration of more differentiated hematopoietic progenitors, that proliferate and differentiate in the liver, and the occurrence of angiogenesis caused by inflammation or release of ovular antigens that stimulate the activation and proliferation of endothelial cells. Altogether, these data increase knowledge about a murine model that is of interest for investigating the pathology of the schistosomiasis and also the dynamics of hematopoiesis. Frontiers Media S.A. 2022-08-25 /pmc/articles/PMC9453041/ /pubmed/36091027 http://dx.doi.org/10.3389/fimmu.2022.955034 Text en Copyright © 2022 Francisco, Terra, Klein, Dias de Oliveira and Pelajo-Machado https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Francisco, Juliane Siqueira
Terra, Marcia Andrea Barge Loução
Klein, Gabriel Couto Thurler
Dias de Oliveira, Barbara Cristina Euzebio Pereira
Pelajo-Machado, Marcelo
The hepatic extramedullary hematopoiesis during experimental murine Schistosomiasis mansoni
title The hepatic extramedullary hematopoiesis during experimental murine Schistosomiasis mansoni
title_full The hepatic extramedullary hematopoiesis during experimental murine Schistosomiasis mansoni
title_fullStr The hepatic extramedullary hematopoiesis during experimental murine Schistosomiasis mansoni
title_full_unstemmed The hepatic extramedullary hematopoiesis during experimental murine Schistosomiasis mansoni
title_short The hepatic extramedullary hematopoiesis during experimental murine Schistosomiasis mansoni
title_sort hepatic extramedullary hematopoiesis during experimental murine schistosomiasis mansoni
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9453041/
https://www.ncbi.nlm.nih.gov/pubmed/36091027
http://dx.doi.org/10.3389/fimmu.2022.955034
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