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How mesenchymal stem cell cotransplantation with hematopoietic stem cells can improve engraftment in animal models

BACKGROUND: Bone marrow transplantation (BMT) can be applied to both hematopoietic and nonhematopoietic diseases; nonetheless, it still comes with a number of challenges and limitations that contribute to treatment failure. Bearing this in mind, a possible way to increase the success rate of BMT wou...

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Autores principales: Garrigós, Murilo Montenegro, de Oliveira, Fernando Anselmo, Nucci, Mariana Penteado, Nucci, Leopoldo Penteado, Alves, Arielly da Hora, Dias, Olivia Furiama Metropolo, Gamarra, Lionel Fernel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Baishideng Publishing Group Inc 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9453272/
https://www.ncbi.nlm.nih.gov/pubmed/36157912
http://dx.doi.org/10.4252/wjsc.v14.i8.658
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author Garrigós, Murilo Montenegro
de Oliveira, Fernando Anselmo
Nucci, Mariana Penteado
Nucci, Leopoldo Penteado
Alves, Arielly da Hora
Dias, Olivia Furiama Metropolo
Gamarra, Lionel Fernel
author_facet Garrigós, Murilo Montenegro
de Oliveira, Fernando Anselmo
Nucci, Mariana Penteado
Nucci, Leopoldo Penteado
Alves, Arielly da Hora
Dias, Olivia Furiama Metropolo
Gamarra, Lionel Fernel
author_sort Garrigós, Murilo Montenegro
collection PubMed
description BACKGROUND: Bone marrow transplantation (BMT) can be applied to both hematopoietic and nonhematopoietic diseases; nonetheless, it still comes with a number of challenges and limitations that contribute to treatment failure. Bearing this in mind, a possible way to increase the success rate of BMT would be cotransplantation of mesenchymal stem cells (MSCs) and hematopoietic stem cells (HSCs) to improve the bone marrow niche and secrete molecules that enhance the hematopoietic engraftment. AIM: To analyze HSC and MSC characteristics and their interactions through cotransplantation in murine models. METHODS: We searched for original articles indexed in PubMed and Scopus during the last decade that used HSC and MSC cotransplantation and in vivo BMT in animal models while evaluating cell engraftment. We excluded in vitro studies or studies that involved graft versus host disease or other hematological diseases and publications in languages other than English. In PubMed, we initially identified 555 articles and after selection, only 12 were chosen. In Scopus, 2010 were identified, and six were left after the screening and eligibility process. RESULTS: Of the 2565 articles found in the databases, only 18 original studies met the eligibility criteria. HSC distribution by source showed similar ratios, with human umbilical cord blood or animal bone marrow being administered mainly with a dose of 1 × 10(7) cells by intravenous or intrabone routes. However, MSCs had a high prevalence of human donors with a variety of sources (umbilical cord blood, bone marrow, tonsil, adipose tissue or fetal lung), using a lower dose, mainly 10(6) cells and ranging 10(4) to 1.5 × 10(7) cells, utilizing the same routes. MSCs were characterized prior to administration in almost every experiment. The recipient used was mostly immunodeficient mice submitted to low-dose irradiation or chemotherapy. The main technique of engraftment for HSC and MSC cotransplantation evaluation was chimerism, followed by hematopoietic reconstitution and survival analysis. Besides the engraftment, homing and cellularity were also evaluated in some studies. CONCLUSION: The preclinical findings validate the potential of MSCs to enable HSC engraftment in vivo in both xenogeneic and allogeneic hematopoietic cell transplantation animal models, in the absence of toxicity.
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spelling pubmed-94532722022-09-23 How mesenchymal stem cell cotransplantation with hematopoietic stem cells can improve engraftment in animal models Garrigós, Murilo Montenegro de Oliveira, Fernando Anselmo Nucci, Mariana Penteado Nucci, Leopoldo Penteado Alves, Arielly da Hora Dias, Olivia Furiama Metropolo Gamarra, Lionel Fernel World J Stem Cells Systematic Reviews BACKGROUND: Bone marrow transplantation (BMT) can be applied to both hematopoietic and nonhematopoietic diseases; nonetheless, it still comes with a number of challenges and limitations that contribute to treatment failure. Bearing this in mind, a possible way to increase the success rate of BMT would be cotransplantation of mesenchymal stem cells (MSCs) and hematopoietic stem cells (HSCs) to improve the bone marrow niche and secrete molecules that enhance the hematopoietic engraftment. AIM: To analyze HSC and MSC characteristics and their interactions through cotransplantation in murine models. METHODS: We searched for original articles indexed in PubMed and Scopus during the last decade that used HSC and MSC cotransplantation and in vivo BMT in animal models while evaluating cell engraftment. We excluded in vitro studies or studies that involved graft versus host disease or other hematological diseases and publications in languages other than English. In PubMed, we initially identified 555 articles and after selection, only 12 were chosen. In Scopus, 2010 were identified, and six were left after the screening and eligibility process. RESULTS: Of the 2565 articles found in the databases, only 18 original studies met the eligibility criteria. HSC distribution by source showed similar ratios, with human umbilical cord blood or animal bone marrow being administered mainly with a dose of 1 × 10(7) cells by intravenous or intrabone routes. However, MSCs had a high prevalence of human donors with a variety of sources (umbilical cord blood, bone marrow, tonsil, adipose tissue or fetal lung), using a lower dose, mainly 10(6) cells and ranging 10(4) to 1.5 × 10(7) cells, utilizing the same routes. MSCs were characterized prior to administration in almost every experiment. The recipient used was mostly immunodeficient mice submitted to low-dose irradiation or chemotherapy. The main technique of engraftment for HSC and MSC cotransplantation evaluation was chimerism, followed by hematopoietic reconstitution and survival analysis. Besides the engraftment, homing and cellularity were also evaluated in some studies. CONCLUSION: The preclinical findings validate the potential of MSCs to enable HSC engraftment in vivo in both xenogeneic and allogeneic hematopoietic cell transplantation animal models, in the absence of toxicity. Baishideng Publishing Group Inc 2022-08-26 2022-08-26 /pmc/articles/PMC9453272/ /pubmed/36157912 http://dx.doi.org/10.4252/wjsc.v14.i8.658 Text en ©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved. https://creativecommons.org/licenses/by-nc/4.0/This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
spellingShingle Systematic Reviews
Garrigós, Murilo Montenegro
de Oliveira, Fernando Anselmo
Nucci, Mariana Penteado
Nucci, Leopoldo Penteado
Alves, Arielly da Hora
Dias, Olivia Furiama Metropolo
Gamarra, Lionel Fernel
How mesenchymal stem cell cotransplantation with hematopoietic stem cells can improve engraftment in animal models
title How mesenchymal stem cell cotransplantation with hematopoietic stem cells can improve engraftment in animal models
title_full How mesenchymal stem cell cotransplantation with hematopoietic stem cells can improve engraftment in animal models
title_fullStr How mesenchymal stem cell cotransplantation with hematopoietic stem cells can improve engraftment in animal models
title_full_unstemmed How mesenchymal stem cell cotransplantation with hematopoietic stem cells can improve engraftment in animal models
title_short How mesenchymal stem cell cotransplantation with hematopoietic stem cells can improve engraftment in animal models
title_sort how mesenchymal stem cell cotransplantation with hematopoietic stem cells can improve engraftment in animal models
topic Systematic Reviews
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9453272/
https://www.ncbi.nlm.nih.gov/pubmed/36157912
http://dx.doi.org/10.4252/wjsc.v14.i8.658
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