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Quercetin positively affects gene expression profiles and metabolic pathway of antibiotic-treated mouse gut microbiota

Quercetin has a wide range of biological properties that can be used to prevent or decrease particular inflammatory diseases. In this study, we aimed to investigate the gene expression profile and metabolic pathway of the gut microbiota of an antibiotic-treated mouse model administered quercetin. Bl...

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Autores principales: Mi, Wei, Hu, Zhiyong, Xu, Lanlan, Bian, Xiangyu, Lian, Wu, Yin, Shuying, Zhao, Shuying, Gao, Weina, Guo, Changjiang, Shi, Tala
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9453598/
https://www.ncbi.nlm.nih.gov/pubmed/36090094
http://dx.doi.org/10.3389/fmicb.2022.983358
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author Mi, Wei
Hu, Zhiyong
Xu, Lanlan
Bian, Xiangyu
Lian, Wu
Yin, Shuying
Zhao, Shuying
Gao, Weina
Guo, Changjiang
Shi, Tala
author_facet Mi, Wei
Hu, Zhiyong
Xu, Lanlan
Bian, Xiangyu
Lian, Wu
Yin, Shuying
Zhao, Shuying
Gao, Weina
Guo, Changjiang
Shi, Tala
author_sort Mi, Wei
collection PubMed
description Quercetin has a wide range of biological properties that can be used to prevent or decrease particular inflammatory diseases. In this study, we aimed to investigate the gene expression profile and metabolic pathway of the gut microbiota of an antibiotic-treated mouse model administered quercetin. Blood, feces, and intestinal tissue samples were collected and metagenomic sequencing, enzyme-linked immunosorbent assay, and western blot analysis were used to detect variations. The results showed that the quercetin-treated group exhibited increased levels of health beneficial bacterial species, including Faecalibaculum rodentium (103.13%), Enterorhabdus caecimuris (4.13%), Eggerthella lenta (4%), Roseburia hominis (1.33%), and Enterorhabdus mucosicola (1.79%), compared with the model group. These bacterial species were positively related to butyrate, propionate, and intestinal tight junction proteins (zonula occludens-1 and occludin) expression, but negatively related to serum lipopolysaccharide and tumor necrosis factor-α level. In addition, the metabolic pathway analysis showed that dietary quercetin significantly enhanced spliceosomes (111.11%), tight junctions (62.96%), the citrate cycle (10.41%), pyruvate metabolism (6.95%), and lysine biosynthesis (5.06%), but decreasing fatty acid biosynthesis (23.91%) and N-glycan (7.37%) biosynthesis. Furthermore, these metabolic pathway changes were related to relative changes in the abundance of 10 Kyoto Encyclopedia of Genes and Genomes genes (K00244, K00341, K02946, K03737, K01885, k10352, k11717, k10532, K02078, K01191). In conclusion, dietary quercetin increased butyrate-producing bacterial species, and the acetyl-CoA-mediated increased butyrate accelerated carbohydrate, energy metabolism, reduced cell motility and endotoxemia, and increased the gut barrier function, thereby leading to healthy colonic conditions for the host.
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spelling pubmed-94535982022-09-09 Quercetin positively affects gene expression profiles and metabolic pathway of antibiotic-treated mouse gut microbiota Mi, Wei Hu, Zhiyong Xu, Lanlan Bian, Xiangyu Lian, Wu Yin, Shuying Zhao, Shuying Gao, Weina Guo, Changjiang Shi, Tala Front Microbiol Microbiology Quercetin has a wide range of biological properties that can be used to prevent or decrease particular inflammatory diseases. In this study, we aimed to investigate the gene expression profile and metabolic pathway of the gut microbiota of an antibiotic-treated mouse model administered quercetin. Blood, feces, and intestinal tissue samples were collected and metagenomic sequencing, enzyme-linked immunosorbent assay, and western blot analysis were used to detect variations. The results showed that the quercetin-treated group exhibited increased levels of health beneficial bacterial species, including Faecalibaculum rodentium (103.13%), Enterorhabdus caecimuris (4.13%), Eggerthella lenta (4%), Roseburia hominis (1.33%), and Enterorhabdus mucosicola (1.79%), compared with the model group. These bacterial species were positively related to butyrate, propionate, and intestinal tight junction proteins (zonula occludens-1 and occludin) expression, but negatively related to serum lipopolysaccharide and tumor necrosis factor-α level. In addition, the metabolic pathway analysis showed that dietary quercetin significantly enhanced spliceosomes (111.11%), tight junctions (62.96%), the citrate cycle (10.41%), pyruvate metabolism (6.95%), and lysine biosynthesis (5.06%), but decreasing fatty acid biosynthesis (23.91%) and N-glycan (7.37%) biosynthesis. Furthermore, these metabolic pathway changes were related to relative changes in the abundance of 10 Kyoto Encyclopedia of Genes and Genomes genes (K00244, K00341, K02946, K03737, K01885, k10352, k11717, k10532, K02078, K01191). In conclusion, dietary quercetin increased butyrate-producing bacterial species, and the acetyl-CoA-mediated increased butyrate accelerated carbohydrate, energy metabolism, reduced cell motility and endotoxemia, and increased the gut barrier function, thereby leading to healthy colonic conditions for the host. Frontiers Media S.A. 2022-08-25 /pmc/articles/PMC9453598/ /pubmed/36090094 http://dx.doi.org/10.3389/fmicb.2022.983358 Text en Copyright © 2022 Mi, Hu, Xu, Bian, Lian, Yin, Zhao, Gao, Guo and Shi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Mi, Wei
Hu, Zhiyong
Xu, Lanlan
Bian, Xiangyu
Lian, Wu
Yin, Shuying
Zhao, Shuying
Gao, Weina
Guo, Changjiang
Shi, Tala
Quercetin positively affects gene expression profiles and metabolic pathway of antibiotic-treated mouse gut microbiota
title Quercetin positively affects gene expression profiles and metabolic pathway of antibiotic-treated mouse gut microbiota
title_full Quercetin positively affects gene expression profiles and metabolic pathway of antibiotic-treated mouse gut microbiota
title_fullStr Quercetin positively affects gene expression profiles and metabolic pathway of antibiotic-treated mouse gut microbiota
title_full_unstemmed Quercetin positively affects gene expression profiles and metabolic pathway of antibiotic-treated mouse gut microbiota
title_short Quercetin positively affects gene expression profiles and metabolic pathway of antibiotic-treated mouse gut microbiota
title_sort quercetin positively affects gene expression profiles and metabolic pathway of antibiotic-treated mouse gut microbiota
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9453598/
https://www.ncbi.nlm.nih.gov/pubmed/36090094
http://dx.doi.org/10.3389/fmicb.2022.983358
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