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The response of cecal microbiota to inflammatory state induced by Salmonella enterica serovar Enteritidis

By combining the experiments of reciprocal crosses of chicken infected with Salmonella enterica serovar Enteritidis (S. Enteritidis), we focused on the common response of cecal microbiota to an inflammatory state in respect of transcriptome and microbiome. The inoculation of S. Enteritidis improved...

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Autores principales: Hu, Geng, Liu, Liying, Miao, Xiuxiu, Zhao, Yanan, Peng, Yanan, Liu, Lewen, Li, Xianyao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9453680/
https://www.ncbi.nlm.nih.gov/pubmed/36090066
http://dx.doi.org/10.3389/fmicb.2022.963678
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author Hu, Geng
Liu, Liying
Miao, Xiuxiu
Zhao, Yanan
Peng, Yanan
Liu, Lewen
Li, Xianyao
author_facet Hu, Geng
Liu, Liying
Miao, Xiuxiu
Zhao, Yanan
Peng, Yanan
Liu, Lewen
Li, Xianyao
author_sort Hu, Geng
collection PubMed
description By combining the experiments of reciprocal crosses of chicken infected with Salmonella enterica serovar Enteritidis (S. Enteritidis), we focused on the common response of cecal microbiota to an inflammatory state in respect of transcriptome and microbiome. The inoculation of S. Enteritidis improved the microbial diversity and promoted the microbiota evolution in our infection model. Correlation analysis between bacteria and inflammation-related genes showed that some intestinal microorganisms were “inflammophile” and thrived in an inflamed environment. The global function of cecal microbiome was to maintain the homeostasis likely by the up-regulation of microbial metabolism pathway in bacitracin, putrescine, and flavonoids production, although the bacitracin may affect the symbiotic bacteria Enterococcus. The action of S. Enteritidis had close relationships with multiple inflammation-related genes, including the genes PTAFR, LY96, and ACOD1 which proteins are related to the binding and tolerance of LPS, and the genes IL-18, IL-18R1 and IL-18RAP which products can form a functional complex and transmit IL-18 pro-inflammatory signal. Additionally, the infection of S. Enteritidis aroused the transcription of EXFABP, which protein has a potential to sequestrate the siderophore and might cause the decline of Escherichia-Shigella and Enterococcus. S. Enteritidis can escape from the sequestrating through the salmochelin, another kind of siderophore which cannot be recognized by EXFABP. Probably by this way, S. Enteritidis competed with the symbiotic bacteria and edged out the niches. Our research can help to understand the interplay between host, pathogen, and symbiotic bacteria.
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spelling pubmed-94536802022-09-09 The response of cecal microbiota to inflammatory state induced by Salmonella enterica serovar Enteritidis Hu, Geng Liu, Liying Miao, Xiuxiu Zhao, Yanan Peng, Yanan Liu, Lewen Li, Xianyao Front Microbiol Microbiology By combining the experiments of reciprocal crosses of chicken infected with Salmonella enterica serovar Enteritidis (S. Enteritidis), we focused on the common response of cecal microbiota to an inflammatory state in respect of transcriptome and microbiome. The inoculation of S. Enteritidis improved the microbial diversity and promoted the microbiota evolution in our infection model. Correlation analysis between bacteria and inflammation-related genes showed that some intestinal microorganisms were “inflammophile” and thrived in an inflamed environment. The global function of cecal microbiome was to maintain the homeostasis likely by the up-regulation of microbial metabolism pathway in bacitracin, putrescine, and flavonoids production, although the bacitracin may affect the symbiotic bacteria Enterococcus. The action of S. Enteritidis had close relationships with multiple inflammation-related genes, including the genes PTAFR, LY96, and ACOD1 which proteins are related to the binding and tolerance of LPS, and the genes IL-18, IL-18R1 and IL-18RAP which products can form a functional complex and transmit IL-18 pro-inflammatory signal. Additionally, the infection of S. Enteritidis aroused the transcription of EXFABP, which protein has a potential to sequestrate the siderophore and might cause the decline of Escherichia-Shigella and Enterococcus. S. Enteritidis can escape from the sequestrating through the salmochelin, another kind of siderophore which cannot be recognized by EXFABP. Probably by this way, S. Enteritidis competed with the symbiotic bacteria and edged out the niches. Our research can help to understand the interplay between host, pathogen, and symbiotic bacteria. Frontiers Media S.A. 2022-08-25 /pmc/articles/PMC9453680/ /pubmed/36090066 http://dx.doi.org/10.3389/fmicb.2022.963678 Text en Copyright © 2022 Hu, Liu, Miao, Zhao, Peng, Liu and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Hu, Geng
Liu, Liying
Miao, Xiuxiu
Zhao, Yanan
Peng, Yanan
Liu, Lewen
Li, Xianyao
The response of cecal microbiota to inflammatory state induced by Salmonella enterica serovar Enteritidis
title The response of cecal microbiota to inflammatory state induced by Salmonella enterica serovar Enteritidis
title_full The response of cecal microbiota to inflammatory state induced by Salmonella enterica serovar Enteritidis
title_fullStr The response of cecal microbiota to inflammatory state induced by Salmonella enterica serovar Enteritidis
title_full_unstemmed The response of cecal microbiota to inflammatory state induced by Salmonella enterica serovar Enteritidis
title_short The response of cecal microbiota to inflammatory state induced by Salmonella enterica serovar Enteritidis
title_sort response of cecal microbiota to inflammatory state induced by salmonella enterica serovar enteritidis
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9453680/
https://www.ncbi.nlm.nih.gov/pubmed/36090066
http://dx.doi.org/10.3389/fmicb.2022.963678
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