Alteration of m(6)A epitranscriptomic tagging of ribonucleic acids after spinal cord injury in mice

The m(6)A methylation is reported to function in multiple physiological and pathological processes. However, the functional relevance of m(6)A modification to post-spinal cord injured (SCI) damage is not yet clear. In the present study, methylated RNA immunoprecipitation combined with microarray ana...

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Autores principales: Ni, Shuangfei, Luo, Zixiang, Fan, Yonggang, Zhang, Weixin, Peng, Wei, Zhang, Huafeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Neuroscience
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9454195/
https://www.ncbi.nlm.nih.gov/pubmed/36090276
http://dx.doi.org/10.3389/fnins.2022.904573
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author Ni, Shuangfei
Luo, Zixiang
Fan, Yonggang
Zhang, Weixin
Peng, Wei
Zhang, Huafeng
author_facet Ni, Shuangfei
Luo, Zixiang
Fan, Yonggang
Zhang, Weixin
Peng, Wei
Zhang, Huafeng
author_sort Ni, Shuangfei
collection PubMed
description The m(6)A methylation is reported to function in multiple physiological and pathological processes. However, the functional relevance of m(6)A modification to post-spinal cord injured (SCI) damage is not yet clear. In the present study, methylated RNA immunoprecipitation combined with microarray analysis showed that the global RNA m(6)A levels were decreased following SCI. Then, gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) analyses were conducted to demonstrate the potential function of differential m(6)A-tagged transcripts and the altered transcripts with differential m(6)A levels. In addition, we found that the m(6)A “writer,” METTL3, significantly decreased after SCI in mice. The immunostaining validated that the expression of METTL3 mainly changed in GFAP or Iba-1(+) cells. Together, this study shows the alteration of m(6)A modification following SCI in mice, which might contribute to the pathophysiology of the spinal cord after trauma.
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spelling pubmed-94541952022-09-09 Alteration of m(6)A epitranscriptomic tagging of ribonucleic acids after spinal cord injury in mice Ni, Shuangfei Luo, Zixiang Fan, Yonggang Zhang, Weixin Peng, Wei Zhang, Huafeng Front Neurosci Neuroscience The m(6)A methylation is reported to function in multiple physiological and pathological processes. However, the functional relevance of m(6)A modification to post-spinal cord injured (SCI) damage is not yet clear. In the present study, methylated RNA immunoprecipitation combined with microarray analysis showed that the global RNA m(6)A levels were decreased following SCI. Then, gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) analyses were conducted to demonstrate the potential function of differential m(6)A-tagged transcripts and the altered transcripts with differential m(6)A levels. In addition, we found that the m(6)A “writer,” METTL3, significantly decreased after SCI in mice. The immunostaining validated that the expression of METTL3 mainly changed in GFAP or Iba-1(+) cells. Together, this study shows the alteration of m(6)A modification following SCI in mice, which might contribute to the pathophysiology of the spinal cord after trauma. Frontiers Media S.A. 2022-08-25 /pmc/articles/PMC9454195/ /pubmed/36090276 http://dx.doi.org/10.3389/fnins.2022.904573 Text en Copyright © 2022 Ni, Luo, Fan, Zhang, Peng and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Ni, Shuangfei
Luo, Zixiang
Fan, Yonggang
Zhang, Weixin
Peng, Wei
Zhang, Huafeng
Alteration of m(6)A epitranscriptomic tagging of ribonucleic acids after spinal cord injury in mice
title Alteration of m(6)A epitranscriptomic tagging of ribonucleic acids after spinal cord injury in mice
title_full Alteration of m(6)A epitranscriptomic tagging of ribonucleic acids after spinal cord injury in mice
title_fullStr Alteration of m(6)A epitranscriptomic tagging of ribonucleic acids after spinal cord injury in mice
title_full_unstemmed Alteration of m(6)A epitranscriptomic tagging of ribonucleic acids after spinal cord injury in mice
title_short Alteration of m(6)A epitranscriptomic tagging of ribonucleic acids after spinal cord injury in mice
title_sort alteration of m(6)a epitranscriptomic tagging of ribonucleic acids after spinal cord injury in mice
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9454195/
https://www.ncbi.nlm.nih.gov/pubmed/36090276
http://dx.doi.org/10.3389/fnins.2022.904573
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