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Multiplex Lateral Flow Assay and the Sample Preparation Method for the Simultaneous Detection of Three Marine Toxins
[Image: see text] A multiplex lateral flow immunoassay (LFA) has been developed to detect the primary marine biotoxin groups: amnesic shellfish poisoning toxins, paralytic shellfish poisoning toxins, and diarrhetic shellfish poisoning toxins. The performance characteristics of the multiplex LFA were...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9454242/ https://www.ncbi.nlm.nih.gov/pubmed/35951987 http://dx.doi.org/10.1021/acs.est.2c02339 |
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author | Mills, Clare Dillon, Michael J. Kulabhusan, Prabir Kumar Senovilla-Herrero, Diana Campbell, Katrina |
author_facet | Mills, Clare Dillon, Michael J. Kulabhusan, Prabir Kumar Senovilla-Herrero, Diana Campbell, Katrina |
author_sort | Mills, Clare |
collection | PubMed |
description | [Image: see text] A multiplex lateral flow immunoassay (LFA) has been developed to detect the primary marine biotoxin groups: amnesic shellfish poisoning toxins, paralytic shellfish poisoning toxins, and diarrhetic shellfish poisoning toxins. The performance characteristics of the multiplex LFA were evaluated for its suitability as a screening method for the detection of toxins in shellfish. The marine toxin-specific antibodies were class-specific, and there was no cross-reactivity between the three toxin groups. The test is capable of detecting all three marine toxin groups, with working ranges of 0.2–1.5, 2.5–65.0, and 8.2–140.3 ng/mL for okadaic acid, saxitoxin, and domoic acid, respectively. This allows the multiplex LFA to detect all three toxin groups at the EU regulatory limits, with a single sample extraction method and dilution volume. No matrix effects were observed on the performance of the LFA with mussel samples spiked with toxins. The developed LFA uses a simple and pocket-sized, portable Cube Reader to provide an accurate result. We also evaluated the use of this Cube Reader with commercially available monoplex lateral flow assays for marine toxins. |
format | Online Article Text |
id | pubmed-9454242 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-94542422022-09-09 Multiplex Lateral Flow Assay and the Sample Preparation Method for the Simultaneous Detection of Three Marine Toxins Mills, Clare Dillon, Michael J. Kulabhusan, Prabir Kumar Senovilla-Herrero, Diana Campbell, Katrina Environ Sci Technol [Image: see text] A multiplex lateral flow immunoassay (LFA) has been developed to detect the primary marine biotoxin groups: amnesic shellfish poisoning toxins, paralytic shellfish poisoning toxins, and diarrhetic shellfish poisoning toxins. The performance characteristics of the multiplex LFA were evaluated for its suitability as a screening method for the detection of toxins in shellfish. The marine toxin-specific antibodies were class-specific, and there was no cross-reactivity between the three toxin groups. The test is capable of detecting all three marine toxin groups, with working ranges of 0.2–1.5, 2.5–65.0, and 8.2–140.3 ng/mL for okadaic acid, saxitoxin, and domoic acid, respectively. This allows the multiplex LFA to detect all three toxin groups at the EU regulatory limits, with a single sample extraction method and dilution volume. No matrix effects were observed on the performance of the LFA with mussel samples spiked with toxins. The developed LFA uses a simple and pocket-sized, portable Cube Reader to provide an accurate result. We also evaluated the use of this Cube Reader with commercially available monoplex lateral flow assays for marine toxins. American Chemical Society 2022-08-11 2022-09-06 /pmc/articles/PMC9454242/ /pubmed/35951987 http://dx.doi.org/10.1021/acs.est.2c02339 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Mills, Clare Dillon, Michael J. Kulabhusan, Prabir Kumar Senovilla-Herrero, Diana Campbell, Katrina Multiplex Lateral Flow Assay and the Sample Preparation Method for the Simultaneous Detection of Three Marine Toxins |
title | Multiplex Lateral
Flow Assay and the Sample Preparation
Method for the Simultaneous Detection of Three Marine Toxins |
title_full | Multiplex Lateral
Flow Assay and the Sample Preparation
Method for the Simultaneous Detection of Three Marine Toxins |
title_fullStr | Multiplex Lateral
Flow Assay and the Sample Preparation
Method for the Simultaneous Detection of Three Marine Toxins |
title_full_unstemmed | Multiplex Lateral
Flow Assay and the Sample Preparation
Method for the Simultaneous Detection of Three Marine Toxins |
title_short | Multiplex Lateral
Flow Assay and the Sample Preparation
Method for the Simultaneous Detection of Three Marine Toxins |
title_sort | multiplex lateral
flow assay and the sample preparation
method for the simultaneous detection of three marine toxins |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9454242/ https://www.ncbi.nlm.nih.gov/pubmed/35951987 http://dx.doi.org/10.1021/acs.est.2c02339 |
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