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Multiplex Lateral Flow Assay and the Sample Preparation Method for the Simultaneous Detection of Three Marine Toxins

[Image: see text] A multiplex lateral flow immunoassay (LFA) has been developed to detect the primary marine biotoxin groups: amnesic shellfish poisoning toxins, paralytic shellfish poisoning toxins, and diarrhetic shellfish poisoning toxins. The performance characteristics of the multiplex LFA were...

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Autores principales: Mills, Clare, Dillon, Michael J., Kulabhusan, Prabir Kumar, Senovilla-Herrero, Diana, Campbell, Katrina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9454242/
https://www.ncbi.nlm.nih.gov/pubmed/35951987
http://dx.doi.org/10.1021/acs.est.2c02339
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author Mills, Clare
Dillon, Michael J.
Kulabhusan, Prabir Kumar
Senovilla-Herrero, Diana
Campbell, Katrina
author_facet Mills, Clare
Dillon, Michael J.
Kulabhusan, Prabir Kumar
Senovilla-Herrero, Diana
Campbell, Katrina
author_sort Mills, Clare
collection PubMed
description [Image: see text] A multiplex lateral flow immunoassay (LFA) has been developed to detect the primary marine biotoxin groups: amnesic shellfish poisoning toxins, paralytic shellfish poisoning toxins, and diarrhetic shellfish poisoning toxins. The performance characteristics of the multiplex LFA were evaluated for its suitability as a screening method for the detection of toxins in shellfish. The marine toxin-specific antibodies were class-specific, and there was no cross-reactivity between the three toxin groups. The test is capable of detecting all three marine toxin groups, with working ranges of 0.2–1.5, 2.5–65.0, and 8.2–140.3 ng/mL for okadaic acid, saxitoxin, and domoic acid, respectively. This allows the multiplex LFA to detect all three toxin groups at the EU regulatory limits, with a single sample extraction method and dilution volume. No matrix effects were observed on the performance of the LFA with mussel samples spiked with toxins. The developed LFA uses a simple and pocket-sized, portable Cube Reader to provide an accurate result. We also evaluated the use of this Cube Reader with commercially available monoplex lateral flow assays for marine toxins.
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spelling pubmed-94542422022-09-09 Multiplex Lateral Flow Assay and the Sample Preparation Method for the Simultaneous Detection of Three Marine Toxins Mills, Clare Dillon, Michael J. Kulabhusan, Prabir Kumar Senovilla-Herrero, Diana Campbell, Katrina Environ Sci Technol [Image: see text] A multiplex lateral flow immunoassay (LFA) has been developed to detect the primary marine biotoxin groups: amnesic shellfish poisoning toxins, paralytic shellfish poisoning toxins, and diarrhetic shellfish poisoning toxins. The performance characteristics of the multiplex LFA were evaluated for its suitability as a screening method for the detection of toxins in shellfish. The marine toxin-specific antibodies were class-specific, and there was no cross-reactivity between the three toxin groups. The test is capable of detecting all three marine toxin groups, with working ranges of 0.2–1.5, 2.5–65.0, and 8.2–140.3 ng/mL for okadaic acid, saxitoxin, and domoic acid, respectively. This allows the multiplex LFA to detect all three toxin groups at the EU regulatory limits, with a single sample extraction method and dilution volume. No matrix effects were observed on the performance of the LFA with mussel samples spiked with toxins. The developed LFA uses a simple and pocket-sized, portable Cube Reader to provide an accurate result. We also evaluated the use of this Cube Reader with commercially available monoplex lateral flow assays for marine toxins. American Chemical Society 2022-08-11 2022-09-06 /pmc/articles/PMC9454242/ /pubmed/35951987 http://dx.doi.org/10.1021/acs.est.2c02339 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Mills, Clare
Dillon, Michael J.
Kulabhusan, Prabir Kumar
Senovilla-Herrero, Diana
Campbell, Katrina
Multiplex Lateral Flow Assay and the Sample Preparation Method for the Simultaneous Detection of Three Marine Toxins
title Multiplex Lateral Flow Assay and the Sample Preparation Method for the Simultaneous Detection of Three Marine Toxins
title_full Multiplex Lateral Flow Assay and the Sample Preparation Method for the Simultaneous Detection of Three Marine Toxins
title_fullStr Multiplex Lateral Flow Assay and the Sample Preparation Method for the Simultaneous Detection of Three Marine Toxins
title_full_unstemmed Multiplex Lateral Flow Assay and the Sample Preparation Method for the Simultaneous Detection of Three Marine Toxins
title_short Multiplex Lateral Flow Assay and the Sample Preparation Method for the Simultaneous Detection of Three Marine Toxins
title_sort multiplex lateral flow assay and the sample preparation method for the simultaneous detection of three marine toxins
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9454242/
https://www.ncbi.nlm.nih.gov/pubmed/35951987
http://dx.doi.org/10.1021/acs.est.2c02339
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