Effects of Essential Oil Blends on In Vitro Apparent and Truly Degradable Dry Matter, Efficiency of Microbial Production, Total Short-Chain Fatty Acids and Greenhouse Gas Emissions of Two Dairy Cow Diets

SIMPLE SUMMARY: Livestock accounts for an estimated 80% of total agricultural greenhouse gas emissions, making abatement of greenhouse gas emissions from livestock a high-priority challenge facing animal nutritionists. Mitigating greenhouse gases in ruminants without reducing animal production is desirable both as a strategy to reduce global greenhouse gas emissions and as a way of improving dietary feed efficiency. The inclusion of feed additives in the diets of ruminants can reduce energy losses as methane, which typically reduces animal performance and contributes to greenhouse gas emissions. The present study evaluated the abatement potential of nine essential oil blends to mitigate greenhouse gas emissions. The inclusion of the blends resulted in a reduction in greenhouse gas emissions and in vitro apparent dry matter digestibility with higher values noted for the control treatment. A similar trend was noted for in vitro truly dry matter digestibility with higher values noted in the control treatment. The efficiency of microbial production was greater for the blends. The inclusion of the blends affected the total and molar proportion of volatile fatty acid concentrations. Overall, inclusion of the blends modified the rumen function resulting in improved efficiency of microbial production. ABSTRACT: The current study evaluated nine essential oil blends (EOBs) for their effects on ruminal in vitro dry matter digestibility (IVDMD), efficiency of microbial production, total short-chain fatty acid concentration (SCFA), total gas, and greenhouse gas (GHG) emissions using two dietary substrates (high forage and high concentrate). The study was arranged as a 2 × 2 × 9 + 1 factorial design to evaluate the effects of the nine EOBs on the two dietary substrates at two time points (6 and 24 h). The inclusion levels of the EOBs were 0 µL (control) and 100 µL with three laboratory replicates. Substrate × EOBs × time interactions were not significant (p > 0.05) for total gas and greenhouse gas emissions. The inclusion of EOBs in the diets resulted in a reduction (p < 0.001) in GHG emissions, except for EOB1 and EOB8 in the high concentrate diet at 6 h and for EOB8 in the high forage diet at 24 h of incubation. Diet type had no effect on apparent IVDMD (IVADMD) whereas the inclusion of EOBs reduced (p < 0.05) IVADMD with higher values noted for the control treatment. The efficiency of microbial production was greater (p < 0.001) for EOB treatments except for EOB1 inclusion in the high forage diet. The inclusion of EOBs affected (p < 0.001) the total and molar proportion of volatile fatty acid concentrations. Overall, the inclusion of the EOBs modified the rumen function resulting in improved efficiency of microbial production. Both the apparent and truly degraded DM was reduced in the EOB treatments. The inclusion of EOBs also resulted in reduced GHG emissions in both diets, except for EOB8 in the high forage diet which was slightly higher than the control treatment.