An Approach for Investigating Sexual Maturity in Wild Boar Males: Testosterone and 17β-Estradiol Analysis

SIMPLE SUMMARY: Wild boar numbers have increased constantly over the last three decades, as have their related conflicts with modern society. For this reason, wild boars have become a frequent topic of discussion. This article describes and explains the concentrations of the two main sexual steroids, testosterone and estradiol, as well as our effort to define threshold levels to recognize the sexual maturation of wild boar males and differentiate between immature and adult boars. With the help of additional analyses on spermatozoa and morphological data, it was shown that prepubertal boars at 8 months of age could be clearly differentiated from postpubertal boars, showing significantly lower concentrations of hormones. Wild boar males older than 11 months of age, in contrast, presented hormonal values like adults. Before the completion of their first year, wild boars are physiologically able to reproduce and may take part in reproduction. The high hormonal concentrations at the end of the breeding season suggest that the mating period could last longer than usually supposed, shifting farrowing into the summer. ABSTRACT: Analyses of sexual steroid hormones in wild boars are rarely described. Testosterone (T) and 17β-estradiol (E2) concentrations are useful to recognize sexual maturation. As threshold values for this species are unknown, additional parameters are required. A total of 127 blood samples from wild boar males were collected to measure T and E2. Age and weight were recorded. Thirty-one epididymides were sampled too. Males were sorted into pre-and postpubertal groups based on the absence/presence of spermatozoa in epididymides and on morphological data following previous results. Forty-four males were prepubertal: the mean age and weight were 10 months and 23 kg, respectively. They showed no spermatozoa. The mean concentrations of T and E2 were 1.2 ± 1.2 ng/mL and 39.7 ± 120.3 pg/mL, respectively. Sixty-six males were postpubertal, twenty-nine of which presented spermatozoa. Their mean concentration of T was 7.6 ± 6.3 ng/mL and E2 was 664.3 ± 250.4 pg/mL. Seventeen samples could not be defined; the hormone concentrations between the two groups suggested a transitional phase consistent with puberty. Wild boars before 12 months of age had high hormone levels like older boars, indicating that they could attempt to reproduce. Hormones at the end of the mating season (January) were high so that reproduction could occur thereafter, shifting farrowing from spring to summer.