Effects of Multi-Strain Probiotics and Perilla frutescens Seed Extract Supplementation Alone or Combined on Growth Performance, Antioxidant Indices, and Intestinal Health of Weaned Piglets

SIMPLE SUMMARY: Weaning piglets face stressors from changes in feed and environment, which affects their growth. To resolve this problem, we explored the separate effects of multi-strain probiotics and Perilla frutescens seed extract and their combined effect on weaning piglets. We found multi-strain probiotics or Perilla frutescens seed extract both improved the gain to feed ratio and antioxidant capacity. In addition, multi-strain probiotics improved jejunal villus height and the villus height/crypt depth ratio. Perilla frutescens seed extract improved ileal villus height. The interactive effects were observed in jejunal villus height and the villus height/crypt depth ratio, ileal villus height, and the gene expression of IL-1β and mucin2 in the intestinal mucosa. This study shows that using either multi-strain probiotics or Perilla frutescens seed extract alone is more effective than their combined use in weaning piglets. ABSTRACT: This study examined the effects of multi-strain probiotics (BL) and Perilla frutescens seed extract (PSE), alone or in combination, on weaning piglets. In total, 96 weaning piglets were allocated into four treatments: CON group (the basal diet), PSE group (basal diet + 1g/kg PSE), BL group (basal diet + 2 g/kg BL), and BL+PSE group (basal diet +1 g/kg PSE + 2 g/kg BL) according to a 2 × 2 factorial arrangement. The supplementation of BL or PSE improved the gain to feed ratio. Dietary BL reduced diarrhea occurrence and Escherichia coli, but increased Lactobacillus counts in the ileal digesta. Dietary PSE tended to increase Lactobacillus counts in the ileal digesta. Interactive effects were found in terms of ileal villus height, the gene expression of IL-1β, and malondialdehyde in the ileal mucosa. Dietary BL lowered malondialdehyde in the spleen, liver, and jejunal mucosa but increased the total antioxidant capacity (T-AOC) in the liver and ileum mucosa. The supplementation of PSE improved superoxide dismutase in serum and T-AOC in the liver, and reduced MDA in liver, spleen, and jejunum mucosa. Taken together, BL or PSE showed positive effects, improving growth and intestinal morphology and enhancing antioxidant capacity. However, their interaction showed no beneficial effects on the antioxidant indices and the intestinal morphology of weaned piglets.