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Drosophila as a Model for Human Viral Neuroinfections

The study of human neurological infection faces many technical and ethical challenges. While not as common as mammalian models, the use of Drosophila (fruit fly) in the investigation of virus–host dynamics is a powerful research tool. In this review, we focus on the benefits and caveats of using Dro...

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Autores principales: Benoit, Ilena, Di Curzio, Domenico, Civetta, Alberto, Douville, Renée N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9454636/
https://www.ncbi.nlm.nih.gov/pubmed/36078091
http://dx.doi.org/10.3390/cells11172685
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author Benoit, Ilena
Di Curzio, Domenico
Civetta, Alberto
Douville, Renée N.
author_facet Benoit, Ilena
Di Curzio, Domenico
Civetta, Alberto
Douville, Renée N.
author_sort Benoit, Ilena
collection PubMed
description The study of human neurological infection faces many technical and ethical challenges. While not as common as mammalian models, the use of Drosophila (fruit fly) in the investigation of virus–host dynamics is a powerful research tool. In this review, we focus on the benefits and caveats of using Drosophila as a model for neurological infections and neuroimmunity. Through the examination of in vitro, in vivo and transgenic systems, we highlight select examples to illustrate the use of flies for the study of exogenous and endogenous viruses associated with neurological disease. In each case, phenotypes in Drosophila are compared to those in human conditions. In addition, we discuss antiviral drug screening in flies and how investigating virus–host interactions may lead to novel antiviral drug targets. Together, we highlight standardized and reproducible readouts of fly behaviour, motor function and neurodegeneration that permit an accurate assessment of neurological outcomes for the study of viral infection in fly models. Adoption of Drosophila as a valuable model system for neurological infections has and will continue to guide the discovery of many novel virus–host interactions.
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spelling pubmed-94546362022-09-09 Drosophila as a Model for Human Viral Neuroinfections Benoit, Ilena Di Curzio, Domenico Civetta, Alberto Douville, Renée N. Cells Review The study of human neurological infection faces many technical and ethical challenges. While not as common as mammalian models, the use of Drosophila (fruit fly) in the investigation of virus–host dynamics is a powerful research tool. In this review, we focus on the benefits and caveats of using Drosophila as a model for neurological infections and neuroimmunity. Through the examination of in vitro, in vivo and transgenic systems, we highlight select examples to illustrate the use of flies for the study of exogenous and endogenous viruses associated with neurological disease. In each case, phenotypes in Drosophila are compared to those in human conditions. In addition, we discuss antiviral drug screening in flies and how investigating virus–host interactions may lead to novel antiviral drug targets. Together, we highlight standardized and reproducible readouts of fly behaviour, motor function and neurodegeneration that permit an accurate assessment of neurological outcomes for the study of viral infection in fly models. Adoption of Drosophila as a valuable model system for neurological infections has and will continue to guide the discovery of many novel virus–host interactions. MDPI 2022-08-29 /pmc/articles/PMC9454636/ /pubmed/36078091 http://dx.doi.org/10.3390/cells11172685 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Benoit, Ilena
Di Curzio, Domenico
Civetta, Alberto
Douville, Renée N.
Drosophila as a Model for Human Viral Neuroinfections
title Drosophila as a Model for Human Viral Neuroinfections
title_full Drosophila as a Model for Human Viral Neuroinfections
title_fullStr Drosophila as a Model for Human Viral Neuroinfections
title_full_unstemmed Drosophila as a Model for Human Viral Neuroinfections
title_short Drosophila as a Model for Human Viral Neuroinfections
title_sort drosophila as a model for human viral neuroinfections
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9454636/
https://www.ncbi.nlm.nih.gov/pubmed/36078091
http://dx.doi.org/10.3390/cells11172685
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