The Role of Ewes’ Udder Health on Echotexture and Blood Flow Changes during the Dry and Lactation Periods

SIMPLE SUMMARY: During lactation, the mammary glands of ewes are prone to infections caused by numerous pathogens. Consequently, mastitis and other udder abnormalities frequently occur. Unfortunately, udder inflammation, whether clinical or subclinical, it poses a significant barrier to livestock profitability by reducing milk production, decreasing milk quality, and suppressing ewes’ reproductive performance. The cost of subclinical mastitis often is greater than that of clinical mastitis. Thus, such conditions must be treated during the dry period, which is important for mammary gland repair and recovery. Moreover, an early diagnosis is crucial for effective treatment. Ultrasonography is a useful tool that is employed in the detection of echotextural and hemodynamic changes. During recent decades, there has been an increasing scientific interest in the contributions of B-mode and Doppler to the determination of physiological and pathological conditions occurring in mammary glands, which cannot be detected during a physical examination. Therefore, the aim of the present study was to investigate the alterations in ewes’ udders’ echotexture and blood flow during the end of lactation, the stages of dry period, and the first days of the postpartum period. A further objective was to investigate the hemodynamic and echotextural differentiations between ewes having healthy udders and ewes having subclinical mastitis. ABSTRACT: The objective of the current study was to investigate the echotextural and hemodynamic changes of ewes entering the dry period with or without subclinical mastitis. B-mode and color Doppler ultrasonography were applied to 12 Chios ewes (6 with healthy udders (group A) and 6 with subclinical mastitis (group B)) before the dry period, during the dry period (the involution phase, steady state, and transition phase), and postpartum. The color Doppler of the mammary arteries was used to evaluate them according to the pulsatility index (PI), resistive index (RI), end-diastolic velocity (EDV), time-averaged maximum velocity (TAMV), blood flow volume (BFV), and artery diameter (D). Udder parenchyma images, analyzed by Echovet v2.0, were used to evaluate the mean value (MV), standard deviation (SD), gradient mean value (GMV), gradient variance (GV), contrast (Con), entropy (Ent), gray value distribution (GVD), run length distribution (RunLD), and long run emphasis (LRunEm). In the involution phase, the PI was higher in group B compared to group A (p ≤ 0.05). The PI and RI were higher postpartum, whereas the EDV, TAVM, and D were higher in the transition phase (p ≤ 0.05). Neither the period nor the ewe group affected the MV, SD, GMV, GV, Con, and GVD values (p ≤ 0.05). In the steady state, the LRunEm was higher in group B, but postpartum, it was higher in group A (p ≤ 0.05). In conclusion, B-mode and Doppler can reveal differences (i) between healthy ewes and ewes with subclinical mastitis and (ii) among the different periods studied. Further research is needed on the blood flow and echotexture indices of the udders of ewes with unilateral subclinical mastitis.