Cargando…
Actin-Related Protein 4 and Linker Histone Sustain Yeast Replicative Ageing
Ageing is accompanied by dramatic changes in chromatin structure organization and genome function. Two essential components of chromatin, the linker histone Hho1p and actin-related protein 4 (Arp4p), have been shown to physically interact in Saccharomyces cerevisiae cells, thus maintaining chromatin...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9454676/ https://www.ncbi.nlm.nih.gov/pubmed/36078161 http://dx.doi.org/10.3390/cells11172754 |
_version_ | 1784785405612130304 |
---|---|
author | Mołoń, Mateusz Stępień, Karolina Kielar, Patrycja Vasileva, Bela Lozanska, Bonka Staneva, Dessislava Ivanov, Penyo Kula-Maximenko, Monika Molestak, Eliza Tchórzewski, Marek Miloshev, George Georgieva, Milena |
author_facet | Mołoń, Mateusz Stępień, Karolina Kielar, Patrycja Vasileva, Bela Lozanska, Bonka Staneva, Dessislava Ivanov, Penyo Kula-Maximenko, Monika Molestak, Eliza Tchórzewski, Marek Miloshev, George Georgieva, Milena |
author_sort | Mołoń, Mateusz |
collection | PubMed |
description | Ageing is accompanied by dramatic changes in chromatin structure organization and genome function. Two essential components of chromatin, the linker histone Hho1p and actin-related protein 4 (Arp4p), have been shown to physically interact in Saccharomyces cerevisiae cells, thus maintaining chromatin dynamics and function, as well as genome stability and cellular morphology. Disrupting this interaction has been proven to influence the stability of the yeast genome and the way cells respond to stress during chronological ageing. It has also been proven that the abrogated interaction between these two chromatin proteins elicited premature ageing phenotypes. Alterations in chromatin compaction have also been associated with replicative ageing, though the main players are not well recognized. Based on this knowledge, here, we examine how the interaction between Hho1p and Arp4p impacts the ageing of mitotically active yeast cells. For this purpose, two sets of strains were used—haploids (WT(n), arp4, hho1Δ and arp4 hho1Δ) and their heterozygous diploid counterparts (WT(2n), ARP4/arp4, HHO1/hho1Δ and ARP4 HHO1/arp4 hho1Δ)—for the performance of extensive morphological and physiological analyses during replicative ageing. These analyses included a comparative examination of the yeast cells’ chromatin structure, proliferative and reproductive potential, and resilience to stress, as well as polysome profiles and chemical composition. The results demonstrated that the haploid chromatin mutants arp4 and arp4 hho1Δ demonstrated a significant reduction in replicative and total lifespan. These findings lead to the conclusion that the importance of a healthy interaction between Arp4p and Hho1p in replicative ageing is significant. This is proof of the concomitant importance of Hho1p and Arp4p in chronological and replicative ageing. |
format | Online Article Text |
id | pubmed-9454676 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94546762022-09-09 Actin-Related Protein 4 and Linker Histone Sustain Yeast Replicative Ageing Mołoń, Mateusz Stępień, Karolina Kielar, Patrycja Vasileva, Bela Lozanska, Bonka Staneva, Dessislava Ivanov, Penyo Kula-Maximenko, Monika Molestak, Eliza Tchórzewski, Marek Miloshev, George Georgieva, Milena Cells Article Ageing is accompanied by dramatic changes in chromatin structure organization and genome function. Two essential components of chromatin, the linker histone Hho1p and actin-related protein 4 (Arp4p), have been shown to physically interact in Saccharomyces cerevisiae cells, thus maintaining chromatin dynamics and function, as well as genome stability and cellular morphology. Disrupting this interaction has been proven to influence the stability of the yeast genome and the way cells respond to stress during chronological ageing. It has also been proven that the abrogated interaction between these two chromatin proteins elicited premature ageing phenotypes. Alterations in chromatin compaction have also been associated with replicative ageing, though the main players are not well recognized. Based on this knowledge, here, we examine how the interaction between Hho1p and Arp4p impacts the ageing of mitotically active yeast cells. For this purpose, two sets of strains were used—haploids (WT(n), arp4, hho1Δ and arp4 hho1Δ) and their heterozygous diploid counterparts (WT(2n), ARP4/arp4, HHO1/hho1Δ and ARP4 HHO1/arp4 hho1Δ)—for the performance of extensive morphological and physiological analyses during replicative ageing. These analyses included a comparative examination of the yeast cells’ chromatin structure, proliferative and reproductive potential, and resilience to stress, as well as polysome profiles and chemical composition. The results demonstrated that the haploid chromatin mutants arp4 and arp4 hho1Δ demonstrated a significant reduction in replicative and total lifespan. These findings lead to the conclusion that the importance of a healthy interaction between Arp4p and Hho1p in replicative ageing is significant. This is proof of the concomitant importance of Hho1p and Arp4p in chronological and replicative ageing. MDPI 2022-09-03 /pmc/articles/PMC9454676/ /pubmed/36078161 http://dx.doi.org/10.3390/cells11172754 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mołoń, Mateusz Stępień, Karolina Kielar, Patrycja Vasileva, Bela Lozanska, Bonka Staneva, Dessislava Ivanov, Penyo Kula-Maximenko, Monika Molestak, Eliza Tchórzewski, Marek Miloshev, George Georgieva, Milena Actin-Related Protein 4 and Linker Histone Sustain Yeast Replicative Ageing |
title | Actin-Related Protein 4 and Linker Histone Sustain Yeast Replicative Ageing |
title_full | Actin-Related Protein 4 and Linker Histone Sustain Yeast Replicative Ageing |
title_fullStr | Actin-Related Protein 4 and Linker Histone Sustain Yeast Replicative Ageing |
title_full_unstemmed | Actin-Related Protein 4 and Linker Histone Sustain Yeast Replicative Ageing |
title_short | Actin-Related Protein 4 and Linker Histone Sustain Yeast Replicative Ageing |
title_sort | actin-related protein 4 and linker histone sustain yeast replicative ageing |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9454676/ https://www.ncbi.nlm.nih.gov/pubmed/36078161 http://dx.doi.org/10.3390/cells11172754 |
work_keys_str_mv | AT mołonmateusz actinrelatedprotein4andlinkerhistonesustainyeastreplicativeageing AT stepienkarolina actinrelatedprotein4andlinkerhistonesustainyeastreplicativeageing AT kielarpatrycja actinrelatedprotein4andlinkerhistonesustainyeastreplicativeageing AT vasilevabela actinrelatedprotein4andlinkerhistonesustainyeastreplicativeageing AT lozanskabonka actinrelatedprotein4andlinkerhistonesustainyeastreplicativeageing AT stanevadessislava actinrelatedprotein4andlinkerhistonesustainyeastreplicativeageing AT ivanovpenyo actinrelatedprotein4andlinkerhistonesustainyeastreplicativeageing AT kulamaximenkomonika actinrelatedprotein4andlinkerhistonesustainyeastreplicativeageing AT molestakeliza actinrelatedprotein4andlinkerhistonesustainyeastreplicativeageing AT tchorzewskimarek actinrelatedprotein4andlinkerhistonesustainyeastreplicativeageing AT miloshevgeorge actinrelatedprotein4andlinkerhistonesustainyeastreplicativeageing AT georgievamilena actinrelatedprotein4andlinkerhistonesustainyeastreplicativeageing |