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Propagation of Babesia bigemina in Rabbit Model and Evaluation of Its Attenuation in Cross-Bred Calves

SIMPLE SUMMARY: Vector-borne diseases (VBDs) cause heavy economic losses in the livestock sector. Among these VBDs, babesiosis is the second most common disease, causing high morbidity, mortality, and reproductive and productive losses in cattle. The causative agents of this disease are globally dis...

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Autores principales: Ullah, Naimat, Ashraf, Kamran, Rehman, Abdul, Suleman, Muhammad, Rashid, Muhammad Imran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9454860/
https://www.ncbi.nlm.nih.gov/pubmed/36078007
http://dx.doi.org/10.3390/ani12172287
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author Ullah, Naimat
Ashraf, Kamran
Rehman, Abdul
Suleman, Muhammad
Rashid, Muhammad Imran
author_facet Ullah, Naimat
Ashraf, Kamran
Rehman, Abdul
Suleman, Muhammad
Rashid, Muhammad Imran
author_sort Ullah, Naimat
collection PubMed
description SIMPLE SUMMARY: Vector-borne diseases (VBDs) cause heavy economic losses in the livestock sector. Among these VBDs, babesiosis is the second most common disease, causing high morbidity, mortality, and reproductive and productive losses in cattle. The causative agents of this disease are globally distributed across tropical and subtropical countries. In the current study, B. bigemina was propagated in vivo in rabbit for attenuation and evaluated for its virulence periodically. This attenuated B. bigemina was then inoculated to naive calves for the evaluation of clinical parameters. Increased parasitaemia and temperature were observed in rabbits following the inoculation of B. bigemina-infected red blood cells. The naive calves did not show symptoms of parasitaemia or temperature elevation when inoculated with rabbit-propagated B. bigemina-infected RBCs. Furthermore, this study also demonstrated that infected cattle periodically had a decreased PCV profile, along with increased temperature and parasitaemia. Moreover, this study also revealed correlations between the temperature, parasitaemia, and packed cell volume of inoculated, infected, and control group calves. ABSTRACT: Bovine babesiosis (BB) is a vector-borne disease (VBD) that affects cattle in tropical and subtropical areas, caused by the haemoprotozoa Babesia bovis and Babesia bigemina. It is transmitted by tick bites belonging to the genus Rhipicephalus and is clinically characterized by high fever, depression, anorexia, decreased milk and meat production, haemoglobinemia, haemoglobinuria, jaundice, and pregnancy loss. In this study, the propagation of B. bigemina was evaluated by intraperitoneally inoculating 3 × 10(6) red blood cells infected with B. bigemina into rabbits. The study showed that variations in rabbit body temperatures are related to induced bovine babesiosis. A significant increase in temperature (39.20 ± 0.23 °C) was observed from day 4 onwards, with the maximum temperature (40.80 ± 1.01 °C) on day 9 post-inoculation. This study included susceptible cross-bred calves for in vivo attenuation, and they were compared with an infected group. The calves in the infected group showed a significant increase in temperature (38.79 ± 0.03 °C) from day 3 onwards and a maximum temperature (41.3 ± 0.17 °C) on day 11. Inoculated calves showed a gradual rise in temperature post-inoculation, but the difference was not significant. Inoculated calves did not show parasitaemia, whereas 32% of infected calves displayed parasitaemia. As compared to inoculated calves post-inoculation, packed cell volume (PCV) decreased (16.36 ± 1.30) for infected calves. However, there were statistically significant differences (p ≤ 0.05) in temperatures, parasitaemia, and PCV in both inoculated and infected calves. The current study aimed to attenuate B. bigemina in rabbit models and evaluate the pathogenic potential of this organism in naive calves. In conclusion, B. bigemina proliferation was attenuated in rabbits. The rabbit model can be used to study B. bigemina in vivo in order to reduce its pathogenicity.
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spelling pubmed-94548602022-09-09 Propagation of Babesia bigemina in Rabbit Model and Evaluation of Its Attenuation in Cross-Bred Calves Ullah, Naimat Ashraf, Kamran Rehman, Abdul Suleman, Muhammad Rashid, Muhammad Imran Animals (Basel) Article SIMPLE SUMMARY: Vector-borne diseases (VBDs) cause heavy economic losses in the livestock sector. Among these VBDs, babesiosis is the second most common disease, causing high morbidity, mortality, and reproductive and productive losses in cattle. The causative agents of this disease are globally distributed across tropical and subtropical countries. In the current study, B. bigemina was propagated in vivo in rabbit for attenuation and evaluated for its virulence periodically. This attenuated B. bigemina was then inoculated to naive calves for the evaluation of clinical parameters. Increased parasitaemia and temperature were observed in rabbits following the inoculation of B. bigemina-infected red blood cells. The naive calves did not show symptoms of parasitaemia or temperature elevation when inoculated with rabbit-propagated B. bigemina-infected RBCs. Furthermore, this study also demonstrated that infected cattle periodically had a decreased PCV profile, along with increased temperature and parasitaemia. Moreover, this study also revealed correlations between the temperature, parasitaemia, and packed cell volume of inoculated, infected, and control group calves. ABSTRACT: Bovine babesiosis (BB) is a vector-borne disease (VBD) that affects cattle in tropical and subtropical areas, caused by the haemoprotozoa Babesia bovis and Babesia bigemina. It is transmitted by tick bites belonging to the genus Rhipicephalus and is clinically characterized by high fever, depression, anorexia, decreased milk and meat production, haemoglobinemia, haemoglobinuria, jaundice, and pregnancy loss. In this study, the propagation of B. bigemina was evaluated by intraperitoneally inoculating 3 × 10(6) red blood cells infected with B. bigemina into rabbits. The study showed that variations in rabbit body temperatures are related to induced bovine babesiosis. A significant increase in temperature (39.20 ± 0.23 °C) was observed from day 4 onwards, with the maximum temperature (40.80 ± 1.01 °C) on day 9 post-inoculation. This study included susceptible cross-bred calves for in vivo attenuation, and they were compared with an infected group. The calves in the infected group showed a significant increase in temperature (38.79 ± 0.03 °C) from day 3 onwards and a maximum temperature (41.3 ± 0.17 °C) on day 11. Inoculated calves showed a gradual rise in temperature post-inoculation, but the difference was not significant. Inoculated calves did not show parasitaemia, whereas 32% of infected calves displayed parasitaemia. As compared to inoculated calves post-inoculation, packed cell volume (PCV) decreased (16.36 ± 1.30) for infected calves. However, there were statistically significant differences (p ≤ 0.05) in temperatures, parasitaemia, and PCV in both inoculated and infected calves. The current study aimed to attenuate B. bigemina in rabbit models and evaluate the pathogenic potential of this organism in naive calves. In conclusion, B. bigemina proliferation was attenuated in rabbits. The rabbit model can be used to study B. bigemina in vivo in order to reduce its pathogenicity. MDPI 2022-09-03 /pmc/articles/PMC9454860/ /pubmed/36078007 http://dx.doi.org/10.3390/ani12172287 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ullah, Naimat
Ashraf, Kamran
Rehman, Abdul
Suleman, Muhammad
Rashid, Muhammad Imran
Propagation of Babesia bigemina in Rabbit Model and Evaluation of Its Attenuation in Cross-Bred Calves
title Propagation of Babesia bigemina in Rabbit Model and Evaluation of Its Attenuation in Cross-Bred Calves
title_full Propagation of Babesia bigemina in Rabbit Model and Evaluation of Its Attenuation in Cross-Bred Calves
title_fullStr Propagation of Babesia bigemina in Rabbit Model and Evaluation of Its Attenuation in Cross-Bred Calves
title_full_unstemmed Propagation of Babesia bigemina in Rabbit Model and Evaluation of Its Attenuation in Cross-Bred Calves
title_short Propagation of Babesia bigemina in Rabbit Model and Evaluation of Its Attenuation in Cross-Bred Calves
title_sort propagation of babesia bigemina in rabbit model and evaluation of its attenuation in cross-bred calves
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9454860/
https://www.ncbi.nlm.nih.gov/pubmed/36078007
http://dx.doi.org/10.3390/ani12172287
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