Apicomplexans in Goat: Prevalence of Neospora caninum, Toxoplasma gondii, Cryptosporidium spp., Eimeria spp. and Risk Factors in Farms from Ecuador

SIMPLE SUMMARY: Neospora caninum, Toxoplasma gondii, Cryptosporidium and Eimeria species are parasites of phylum Apicomplexa, which includes several protozoa affecting animals and humans. In Ecuador, the maintenance of goat health is a matter of utmost importance because it affects the economic welfare of the breeders. N. caninum and T. gondii cause reproductive problems in goats, leading to abortions or weak offspring. Severe diarrhea in kids and delays in growth are due to the Cryptosporidium and Eimeria species. Moreover, T. gondii and Cryptosporidium are zoonotic parasites with serious consequences for human health. The aim of this work was to determine, by serological and parasitological tests, the prevalence of these parasites and the risk factors for goat populations in Southern Ecuador. On some farms, the prevalence of N. caninum and T. gondii reached more than 50%; up to 17% of the kids were positive for Cryptosporidium and 90% of the goats were positive for the Eimeria species. The analysis of risk factors revealed differences according to the parasite species. Considering the zoonotic significance of these results, control and prevention measures are essential and constitute a warning to veterinarians and governmental institutions. ABSTRACT: Neospora caninum, Toxoplasma gondii, Cryptosporidium and Eimeria cause severe impacts on the productivity of goat herds. The objectives of the present study were to establish the prevalence of these apicomplexans in goat farms from Ecuador; to evaluate a rapid test for Cryptosporidium diagnosis and to identify the risk factors associated with the infections. A questionnaire was designed to obtain information from 24 goat farms from Zapotillo, Garza Real, Cazaderos, Limones and Paletillas parishes in Ecuador. Blood (n = 388) and feces (n = 391) samples were collected. Indirect ELISA and standard parasitological assays were carried out to evaluate the seroprevalence of N. caninum and T. gondii and to detect oocysts of Cryptosporidium and Eimeria. The overall prevalence values of N. caninum and T. gondii were 12.11% and 18.20%, Cryptosporidium spp. and Eimeria spp. oocysts were detected in 10.49% and 89.51% of the total samples. A low correlation value was found between the results obtained by Ziehl-Nielsen and the rapid test. The multinomial logistic regression analysis revealed that vitamin supplementation, age of diarrhea, frequency of deworming, pasture area, presence of artiodactyls, domestic fowl, administration of sulfas, age group, body condition, abortions, type of pastures and the presence of cattle were risk factors according to the parasite species.