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New data analysis for BioSAXS at the ESRF
The second phase of the ESRF upgrade program did not only provide a new storage ring (Extremely Brilliant Source, EBS) but also allowed several beamlines to be refurbished. The BioSAXS beamline (located on port BM29) was upgraded with a new wiggler source and a larger detector. All analysis software...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9455220/ https://www.ncbi.nlm.nih.gov/pubmed/36073892 http://dx.doi.org/10.1107/S1600577522007238 |
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author | Kieffer, Jérôme Brennich, Martha Florial, Jean-Baptiste Oscarsson, Marcus De Maria Antolinos, Alejandro Tully, Mark Pernot, Petra |
author_facet | Kieffer, Jérôme Brennich, Martha Florial, Jean-Baptiste Oscarsson, Marcus De Maria Antolinos, Alejandro Tully, Mark Pernot, Petra |
author_sort | Kieffer, Jérôme |
collection | PubMed |
description | The second phase of the ESRF upgrade program did not only provide a new storage ring (Extremely Brilliant Source, EBS) but also allowed several beamlines to be refurbished. The BioSAXS beamline (located on port BM29) was upgraded with a new wiggler source and a larger detector. All analysis software has been rewritten to cope with the increased data flux and continues to provide beamline users with reduced and pre-processed data in real time. This article describes FreeSAS, an open-source collection of various small-angle scattering analysis algorithms needed to reduce and analyze BioSAXS data, and Dahu, the tool used to interface data analysis with beamline control. It further presents the data-processing pipelines for the different data acquisitions modes of the beamline, using either a sample changer for individual homogeneous samples or an inline size-exclusion chromatography setup. |
format | Online Article Text |
id | pubmed-9455220 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-94552202022-10-03 New data analysis for BioSAXS at the ESRF Kieffer, Jérôme Brennich, Martha Florial, Jean-Baptiste Oscarsson, Marcus De Maria Antolinos, Alejandro Tully, Mark Pernot, Petra J Synchrotron Radiat Computer Programs The second phase of the ESRF upgrade program did not only provide a new storage ring (Extremely Brilliant Source, EBS) but also allowed several beamlines to be refurbished. The BioSAXS beamline (located on port BM29) was upgraded with a new wiggler source and a larger detector. All analysis software has been rewritten to cope with the increased data flux and continues to provide beamline users with reduced and pre-processed data in real time. This article describes FreeSAS, an open-source collection of various small-angle scattering analysis algorithms needed to reduce and analyze BioSAXS data, and Dahu, the tool used to interface data analysis with beamline control. It further presents the data-processing pipelines for the different data acquisitions modes of the beamline, using either a sample changer for individual homogeneous samples or an inline size-exclusion chromatography setup. International Union of Crystallography 2022-08-23 /pmc/articles/PMC9455220/ /pubmed/36073892 http://dx.doi.org/10.1107/S1600577522007238 Text en © Jérôme Kieffer et al. 2022 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
spellingShingle | Computer Programs Kieffer, Jérôme Brennich, Martha Florial, Jean-Baptiste Oscarsson, Marcus De Maria Antolinos, Alejandro Tully, Mark Pernot, Petra New data analysis for BioSAXS at the ESRF |
title | New data analysis for BioSAXS at the ESRF |
title_full | New data analysis for BioSAXS at the ESRF |
title_fullStr | New data analysis for BioSAXS at the ESRF |
title_full_unstemmed | New data analysis for BioSAXS at the ESRF |
title_short | New data analysis for BioSAXS at the ESRF |
title_sort | new data analysis for biosaxs at the esrf |
topic | Computer Programs |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9455220/ https://www.ncbi.nlm.nih.gov/pubmed/36073892 http://dx.doi.org/10.1107/S1600577522007238 |
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