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Bacteriocin Production by Escherichia coli during Biofilm Development

Escherichia coli is a highly versatile bacterium ranging from commensal to intestinal pathogen, and is an important foodborne pathogen. E. coli species are able to prosper in multispecies biofilms and secrete bacteriocins that are only toxic to species/strains closely related to the producer strain....

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Autores principales: Fokt, Hanna, Cleto, Sara, Oliveira, Hugo, Araújo, Daniela, Castro, Joana, Cerca, Nuno, Vieira, Maria João, Almeida, Carina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9455227/
https://www.ncbi.nlm.nih.gov/pubmed/36076837
http://dx.doi.org/10.3390/foods11172652
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author Fokt, Hanna
Cleto, Sara
Oliveira, Hugo
Araújo, Daniela
Castro, Joana
Cerca, Nuno
Vieira, Maria João
Almeida, Carina
author_facet Fokt, Hanna
Cleto, Sara
Oliveira, Hugo
Araújo, Daniela
Castro, Joana
Cerca, Nuno
Vieira, Maria João
Almeida, Carina
author_sort Fokt, Hanna
collection PubMed
description Escherichia coli is a highly versatile bacterium ranging from commensal to intestinal pathogen, and is an important foodborne pathogen. E. coli species are able to prosper in multispecies biofilms and secrete bacteriocins that are only toxic to species/strains closely related to the producer strain. In this study, 20 distinct E. coli strains were characterized for several properties that confer competitive advantages against closer microorganisms by assessing the biofilm-forming capacity, the production of antimicrobial molecules, and the production of siderophores. Furthermore, primer sets for E. coli bacteriocins–colicins were designed and genes were amplified, allowing us to observe that colicins were widely distributed among the pathogenic E. coli strains. Their production in the planktonic phase or single-species biofilms was uncommon. Only two E. coli strains out of nine biofilm-forming were able to inhibit the growth of other E. coli strains. There is evidence of larger amounts of colicin being produced in the late stages of E. coli biofilm growth. The decrease in bacterial biomass after 12 h of incubation indicates active type I colicin production, whose release normally requires E. coli cell lysis. Almost all E. coli strains were siderophore-producing, which may be related to the resistance to colicin as these two molecules may use the same transporter system. Moreover, E. coli CECT 504 was able to coexist with Salmonella enterica in dual-species biofilms, but Shigella dysenteriae was selectively excluded, correlating with high expression levels of colicin (E, B, and M) genes observed by real-time PCR.
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spelling pubmed-94552272022-09-09 Bacteriocin Production by Escherichia coli during Biofilm Development Fokt, Hanna Cleto, Sara Oliveira, Hugo Araújo, Daniela Castro, Joana Cerca, Nuno Vieira, Maria João Almeida, Carina Foods Article Escherichia coli is a highly versatile bacterium ranging from commensal to intestinal pathogen, and is an important foodborne pathogen. E. coli species are able to prosper in multispecies biofilms and secrete bacteriocins that are only toxic to species/strains closely related to the producer strain. In this study, 20 distinct E. coli strains were characterized for several properties that confer competitive advantages against closer microorganisms by assessing the biofilm-forming capacity, the production of antimicrobial molecules, and the production of siderophores. Furthermore, primer sets for E. coli bacteriocins–colicins were designed and genes were amplified, allowing us to observe that colicins were widely distributed among the pathogenic E. coli strains. Their production in the planktonic phase or single-species biofilms was uncommon. Only two E. coli strains out of nine biofilm-forming were able to inhibit the growth of other E. coli strains. There is evidence of larger amounts of colicin being produced in the late stages of E. coli biofilm growth. The decrease in bacterial biomass after 12 h of incubation indicates active type I colicin production, whose release normally requires E. coli cell lysis. Almost all E. coli strains were siderophore-producing, which may be related to the resistance to colicin as these two molecules may use the same transporter system. Moreover, E. coli CECT 504 was able to coexist with Salmonella enterica in dual-species biofilms, but Shigella dysenteriae was selectively excluded, correlating with high expression levels of colicin (E, B, and M) genes observed by real-time PCR. MDPI 2022-09-01 /pmc/articles/PMC9455227/ /pubmed/36076837 http://dx.doi.org/10.3390/foods11172652 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fokt, Hanna
Cleto, Sara
Oliveira, Hugo
Araújo, Daniela
Castro, Joana
Cerca, Nuno
Vieira, Maria João
Almeida, Carina
Bacteriocin Production by Escherichia coli during Biofilm Development
title Bacteriocin Production by Escherichia coli during Biofilm Development
title_full Bacteriocin Production by Escherichia coli during Biofilm Development
title_fullStr Bacteriocin Production by Escherichia coli during Biofilm Development
title_full_unstemmed Bacteriocin Production by Escherichia coli during Biofilm Development
title_short Bacteriocin Production by Escherichia coli during Biofilm Development
title_sort bacteriocin production by escherichia coli during biofilm development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9455227/
https://www.ncbi.nlm.nih.gov/pubmed/36076837
http://dx.doi.org/10.3390/foods11172652
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