Quantitative Detection of Viable but Nonculturable Cronobacter sakazakii Using Photosensitive Nucleic Acid Dye PMA Combined with Isothermal Amplification LAMP in Raw Milk

An accurate method that rapidly detects the number of viable but nonculturable (VBNC) Cronobacter sakazakii was developed by combining propidium bromide with quantitative LAMP (PMA-QLAMP). The gyrB gene was the target for primers design. The optimal PMA treatment conditions were determined to elimin...

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Detalles Bibliográficos
Autores principales: Hu, Lianxia, Zhang, Shufei, Xue, Yuling, Zhang, Yaoguang, Zhang, Wei, Wang, Shijie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9455467/
https://www.ncbi.nlm.nih.gov/pubmed/36076838
http://dx.doi.org/10.3390/foods11172653

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