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The microbiome of lower respiratory tract and tumor tissue in lung cancer manifested as radiological ground-glass opacity
Recent studies have confirmed the existence of microbiota in the lungs. The relationship between lung ground-glass opacity (GGO) and microbiota in the lung microenvironment is not clear. In this study, we investigated the microbial composition and diversity in bronchoalveolar lavage fluid (BALF) of...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9455596/ https://www.ncbi.nlm.nih.gov/pubmed/36091439 http://dx.doi.org/10.3389/fbioe.2022.892613 |
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author | Wu, Zhigang Tang, Jie Zhuang, Runzhou Meng, Di Zhang, Lichen Gu, Chen Teng, Xiao Zhu, Ziyue Liu, Jiacong Pang, Jinghua Hu, Jian Lv, Xiayi |
author_facet | Wu, Zhigang Tang, Jie Zhuang, Runzhou Meng, Di Zhang, Lichen Gu, Chen Teng, Xiao Zhu, Ziyue Liu, Jiacong Pang, Jinghua Hu, Jian Lv, Xiayi |
author_sort | Wu, Zhigang |
collection | PubMed |
description | Recent studies have confirmed the existence of microbiota in the lungs. The relationship between lung ground-glass opacity (GGO) and microbiota in the lung microenvironment is not clear. In this study, we investigated the microbial composition and diversity in bronchoalveolar lavage fluid (BALF) of diseased lung segments and paired contralateral healthy lung segments from 11 GGO patients. Furthermore, lung GGO and paired normal tissues of 26 GGO patients were explored whether there are microbial characteristics related to GGO. Compared with the control group, the community richness of GGO tissue and BALF of GGO lung segment (α-diversity) and overall microbiome difference (β-diversity) had no significant difference. The microbiome composition of BALF of GGO segments is distinct from that of paired healthy lung segments [genus (Rothia), order (Lachnospiraceae), family (Lachnospiraceae), genus (Lachnospiraceae_NK4A136_group, Faecalibacterium), and species (Faecalibacterium prausnitzii, Bacteroides uniforms)]. GGO tissue and adjacent lung tissue had more significant differences at the levels of class, order, family, genus, and species level, and most of them are enriched in normal lung tissue. The area under the curve (AUC) using 10 genera-based biomarkers to predict GGO was 91.05% (95% CI: 81.93–100%). In conclusion, this study demonstrates there are significant differences in the lower respiratory tract and lung microbiome between GGO and the non-malignant control group through the BALF and lung tissues. Furthermore, some potential bacterial biomarkers showed good performance to predict GGO. |
format | Online Article Text |
id | pubmed-9455596 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94555962022-09-09 The microbiome of lower respiratory tract and tumor tissue in lung cancer manifested as radiological ground-glass opacity Wu, Zhigang Tang, Jie Zhuang, Runzhou Meng, Di Zhang, Lichen Gu, Chen Teng, Xiao Zhu, Ziyue Liu, Jiacong Pang, Jinghua Hu, Jian Lv, Xiayi Front Bioeng Biotechnol Bioengineering and Biotechnology Recent studies have confirmed the existence of microbiota in the lungs. The relationship between lung ground-glass opacity (GGO) and microbiota in the lung microenvironment is not clear. In this study, we investigated the microbial composition and diversity in bronchoalveolar lavage fluid (BALF) of diseased lung segments and paired contralateral healthy lung segments from 11 GGO patients. Furthermore, lung GGO and paired normal tissues of 26 GGO patients were explored whether there are microbial characteristics related to GGO. Compared with the control group, the community richness of GGO tissue and BALF of GGO lung segment (α-diversity) and overall microbiome difference (β-diversity) had no significant difference. The microbiome composition of BALF of GGO segments is distinct from that of paired healthy lung segments [genus (Rothia), order (Lachnospiraceae), family (Lachnospiraceae), genus (Lachnospiraceae_NK4A136_group, Faecalibacterium), and species (Faecalibacterium prausnitzii, Bacteroides uniforms)]. GGO tissue and adjacent lung tissue had more significant differences at the levels of class, order, family, genus, and species level, and most of them are enriched in normal lung tissue. The area under the curve (AUC) using 10 genera-based biomarkers to predict GGO was 91.05% (95% CI: 81.93–100%). In conclusion, this study demonstrates there are significant differences in the lower respiratory tract and lung microbiome between GGO and the non-malignant control group through the BALF and lung tissues. Furthermore, some potential bacterial biomarkers showed good performance to predict GGO. Frontiers Media S.A. 2022-08-25 /pmc/articles/PMC9455596/ /pubmed/36091439 http://dx.doi.org/10.3389/fbioe.2022.892613 Text en Copyright © 2022 Wu, Tang, Zhuang, Meng, Zhang, Gu, Teng, Zhu, Liu, Pang, Hu and Lv. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Wu, Zhigang Tang, Jie Zhuang, Runzhou Meng, Di Zhang, Lichen Gu, Chen Teng, Xiao Zhu, Ziyue Liu, Jiacong Pang, Jinghua Hu, Jian Lv, Xiayi The microbiome of lower respiratory tract and tumor tissue in lung cancer manifested as radiological ground-glass opacity |
title | The microbiome of lower respiratory tract and tumor tissue in lung cancer manifested as radiological ground-glass opacity |
title_full | The microbiome of lower respiratory tract and tumor tissue in lung cancer manifested as radiological ground-glass opacity |
title_fullStr | The microbiome of lower respiratory tract and tumor tissue in lung cancer manifested as radiological ground-glass opacity |
title_full_unstemmed | The microbiome of lower respiratory tract and tumor tissue in lung cancer manifested as radiological ground-glass opacity |
title_short | The microbiome of lower respiratory tract and tumor tissue in lung cancer manifested as radiological ground-glass opacity |
title_sort | microbiome of lower respiratory tract and tumor tissue in lung cancer manifested as radiological ground-glass opacity |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9455596/ https://www.ncbi.nlm.nih.gov/pubmed/36091439 http://dx.doi.org/10.3389/fbioe.2022.892613 |
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