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The microbiome of lower respiratory tract and tumor tissue in lung cancer manifested as radiological ground-glass opacity

Recent studies have confirmed the existence of microbiota in the lungs. The relationship between lung ground-glass opacity (GGO) and microbiota in the lung microenvironment is not clear. In this study, we investigated the microbial composition and diversity in bronchoalveolar lavage fluid (BALF) of...

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Autores principales: Wu, Zhigang, Tang, Jie, Zhuang, Runzhou, Meng, Di, Zhang, Lichen, Gu, Chen, Teng, Xiao, Zhu, Ziyue, Liu, Jiacong, Pang, Jinghua, Hu, Jian, Lv, Xiayi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9455596/
https://www.ncbi.nlm.nih.gov/pubmed/36091439
http://dx.doi.org/10.3389/fbioe.2022.892613
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author Wu, Zhigang
Tang, Jie
Zhuang, Runzhou
Meng, Di
Zhang, Lichen
Gu, Chen
Teng, Xiao
Zhu, Ziyue
Liu, Jiacong
Pang, Jinghua
Hu, Jian
Lv, Xiayi
author_facet Wu, Zhigang
Tang, Jie
Zhuang, Runzhou
Meng, Di
Zhang, Lichen
Gu, Chen
Teng, Xiao
Zhu, Ziyue
Liu, Jiacong
Pang, Jinghua
Hu, Jian
Lv, Xiayi
author_sort Wu, Zhigang
collection PubMed
description Recent studies have confirmed the existence of microbiota in the lungs. The relationship between lung ground-glass opacity (GGO) and microbiota in the lung microenvironment is not clear. In this study, we investigated the microbial composition and diversity in bronchoalveolar lavage fluid (BALF) of diseased lung segments and paired contralateral healthy lung segments from 11 GGO patients. Furthermore, lung GGO and paired normal tissues of 26 GGO patients were explored whether there are microbial characteristics related to GGO. Compared with the control group, the community richness of GGO tissue and BALF of GGO lung segment (α-diversity) and overall microbiome difference (β-diversity) had no significant difference. The microbiome composition of BALF of GGO segments is distinct from that of paired healthy lung segments [genus (Rothia), order (Lachnospiraceae), family (Lachnospiraceae), genus (Lachnospiraceae_NK4A136_group, Faecalibacterium), and species (Faecalibacterium prausnitzii, Bacteroides uniforms)]. GGO tissue and adjacent lung tissue had more significant differences at the levels of class, order, family, genus, and species level, and most of them are enriched in normal lung tissue. The area under the curve (AUC) using 10 genera-based biomarkers to predict GGO was 91.05% (95% CI: 81.93–100%). In conclusion, this study demonstrates there are significant differences in the lower respiratory tract and lung microbiome between GGO and the non-malignant control group through the BALF and lung tissues. Furthermore, some potential bacterial biomarkers showed good performance to predict GGO.
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spelling pubmed-94555962022-09-09 The microbiome of lower respiratory tract and tumor tissue in lung cancer manifested as radiological ground-glass opacity Wu, Zhigang Tang, Jie Zhuang, Runzhou Meng, Di Zhang, Lichen Gu, Chen Teng, Xiao Zhu, Ziyue Liu, Jiacong Pang, Jinghua Hu, Jian Lv, Xiayi Front Bioeng Biotechnol Bioengineering and Biotechnology Recent studies have confirmed the existence of microbiota in the lungs. The relationship between lung ground-glass opacity (GGO) and microbiota in the lung microenvironment is not clear. In this study, we investigated the microbial composition and diversity in bronchoalveolar lavage fluid (BALF) of diseased lung segments and paired contralateral healthy lung segments from 11 GGO patients. Furthermore, lung GGO and paired normal tissues of 26 GGO patients were explored whether there are microbial characteristics related to GGO. Compared with the control group, the community richness of GGO tissue and BALF of GGO lung segment (α-diversity) and overall microbiome difference (β-diversity) had no significant difference. The microbiome composition of BALF of GGO segments is distinct from that of paired healthy lung segments [genus (Rothia), order (Lachnospiraceae), family (Lachnospiraceae), genus (Lachnospiraceae_NK4A136_group, Faecalibacterium), and species (Faecalibacterium prausnitzii, Bacteroides uniforms)]. GGO tissue and adjacent lung tissue had more significant differences at the levels of class, order, family, genus, and species level, and most of them are enriched in normal lung tissue. The area under the curve (AUC) using 10 genera-based biomarkers to predict GGO was 91.05% (95% CI: 81.93–100%). In conclusion, this study demonstrates there are significant differences in the lower respiratory tract and lung microbiome between GGO and the non-malignant control group through the BALF and lung tissues. Furthermore, some potential bacterial biomarkers showed good performance to predict GGO. Frontiers Media S.A. 2022-08-25 /pmc/articles/PMC9455596/ /pubmed/36091439 http://dx.doi.org/10.3389/fbioe.2022.892613 Text en Copyright © 2022 Wu, Tang, Zhuang, Meng, Zhang, Gu, Teng, Zhu, Liu, Pang, Hu and Lv. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Wu, Zhigang
Tang, Jie
Zhuang, Runzhou
Meng, Di
Zhang, Lichen
Gu, Chen
Teng, Xiao
Zhu, Ziyue
Liu, Jiacong
Pang, Jinghua
Hu, Jian
Lv, Xiayi
The microbiome of lower respiratory tract and tumor tissue in lung cancer manifested as radiological ground-glass opacity
title The microbiome of lower respiratory tract and tumor tissue in lung cancer manifested as radiological ground-glass opacity
title_full The microbiome of lower respiratory tract and tumor tissue in lung cancer manifested as radiological ground-glass opacity
title_fullStr The microbiome of lower respiratory tract and tumor tissue in lung cancer manifested as radiological ground-glass opacity
title_full_unstemmed The microbiome of lower respiratory tract and tumor tissue in lung cancer manifested as radiological ground-glass opacity
title_short The microbiome of lower respiratory tract and tumor tissue in lung cancer manifested as radiological ground-glass opacity
title_sort microbiome of lower respiratory tract and tumor tissue in lung cancer manifested as radiological ground-glass opacity
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9455596/
https://www.ncbi.nlm.nih.gov/pubmed/36091439
http://dx.doi.org/10.3389/fbioe.2022.892613
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