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Biomimetic Keratin-Coated Gold Nanoparticles for Photo-Thermal Therapy in a 3D Bioprinted Glioblastoma Tumor Model

Before entering human clinical studies to evaluate their safety and effectiveness, new drugs and novel medical treatments are subject to extensive animal testing that are expensive and time-consuming. By contrast, advanced technologies enable the development of animal-free models that allow the effi...

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Autores principales: Chirivì, Maila, Bearzi, Claudia, Rosa, Paolo, Miglietta, Selenia, Petronella, Francesca, De Falco, Elena, Calogero, Antonella, Pani, Roberto, Petrozza, Vincenzo, Perotto, Giovanni, Rizzi, Roberto, De Sio, Luciano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9455633/
https://www.ncbi.nlm.nih.gov/pubmed/36076927
http://dx.doi.org/10.3390/ijms23179528
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author Chirivì, Maila
Bearzi, Claudia
Rosa, Paolo
Miglietta, Selenia
Petronella, Francesca
De Falco, Elena
Calogero, Antonella
Pani, Roberto
Petrozza, Vincenzo
Perotto, Giovanni
Rizzi, Roberto
De Sio, Luciano
author_facet Chirivì, Maila
Bearzi, Claudia
Rosa, Paolo
Miglietta, Selenia
Petronella, Francesca
De Falco, Elena
Calogero, Antonella
Pani, Roberto
Petrozza, Vincenzo
Perotto, Giovanni
Rizzi, Roberto
De Sio, Luciano
author_sort Chirivì, Maila
collection PubMed
description Before entering human clinical studies to evaluate their safety and effectiveness, new drugs and novel medical treatments are subject to extensive animal testing that are expensive and time-consuming. By contrast, advanced technologies enable the development of animal-free models that allow the efficacy of innovative therapies to be studied without sacrificing animals, while providing helpful information and details. We report on the powerful combination of 3D bioprinting (3DB) and photo-thermal therapy (PTT) applications. To this end, we realize a 3DB construct consisting of glioblastoma U87-MG cells in a 3D geometry, incorporating biomimetic keratin-coated gold nanoparticles (Ker-AuNPs) as a photo-thermal agent. The resulting plasmonic 3DB structures exhibit a homogeneous cell distribution throughout the entire volume while promoting the localization of Ker-AuNPs within the cells. A 3D immunofluorescence assay and transmission electron microscopy (TEM) confirm the uniform distribution of fluorescent-labeled Ker-AuNPs in the volume and their capability to enter the cells. Laser-assisted (λ = 532 nm) PTT experiments demonstrate the extraordinary ability of Ker-AuNPs to generate heating, producing the highest temperature rise of about 16 °C in less than 2 min.
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spelling pubmed-94556332022-09-09 Biomimetic Keratin-Coated Gold Nanoparticles for Photo-Thermal Therapy in a 3D Bioprinted Glioblastoma Tumor Model Chirivì, Maila Bearzi, Claudia Rosa, Paolo Miglietta, Selenia Petronella, Francesca De Falco, Elena Calogero, Antonella Pani, Roberto Petrozza, Vincenzo Perotto, Giovanni Rizzi, Roberto De Sio, Luciano Int J Mol Sci Article Before entering human clinical studies to evaluate their safety and effectiveness, new drugs and novel medical treatments are subject to extensive animal testing that are expensive and time-consuming. By contrast, advanced technologies enable the development of animal-free models that allow the efficacy of innovative therapies to be studied without sacrificing animals, while providing helpful information and details. We report on the powerful combination of 3D bioprinting (3DB) and photo-thermal therapy (PTT) applications. To this end, we realize a 3DB construct consisting of glioblastoma U87-MG cells in a 3D geometry, incorporating biomimetic keratin-coated gold nanoparticles (Ker-AuNPs) as a photo-thermal agent. The resulting plasmonic 3DB structures exhibit a homogeneous cell distribution throughout the entire volume while promoting the localization of Ker-AuNPs within the cells. A 3D immunofluorescence assay and transmission electron microscopy (TEM) confirm the uniform distribution of fluorescent-labeled Ker-AuNPs in the volume and their capability to enter the cells. Laser-assisted (λ = 532 nm) PTT experiments demonstrate the extraordinary ability of Ker-AuNPs to generate heating, producing the highest temperature rise of about 16 °C in less than 2 min. MDPI 2022-08-23 /pmc/articles/PMC9455633/ /pubmed/36076927 http://dx.doi.org/10.3390/ijms23179528 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chirivì, Maila
Bearzi, Claudia
Rosa, Paolo
Miglietta, Selenia
Petronella, Francesca
De Falco, Elena
Calogero, Antonella
Pani, Roberto
Petrozza, Vincenzo
Perotto, Giovanni
Rizzi, Roberto
De Sio, Luciano
Biomimetic Keratin-Coated Gold Nanoparticles for Photo-Thermal Therapy in a 3D Bioprinted Glioblastoma Tumor Model
title Biomimetic Keratin-Coated Gold Nanoparticles for Photo-Thermal Therapy in a 3D Bioprinted Glioblastoma Tumor Model
title_full Biomimetic Keratin-Coated Gold Nanoparticles for Photo-Thermal Therapy in a 3D Bioprinted Glioblastoma Tumor Model
title_fullStr Biomimetic Keratin-Coated Gold Nanoparticles for Photo-Thermal Therapy in a 3D Bioprinted Glioblastoma Tumor Model
title_full_unstemmed Biomimetic Keratin-Coated Gold Nanoparticles for Photo-Thermal Therapy in a 3D Bioprinted Glioblastoma Tumor Model
title_short Biomimetic Keratin-Coated Gold Nanoparticles for Photo-Thermal Therapy in a 3D Bioprinted Glioblastoma Tumor Model
title_sort biomimetic keratin-coated gold nanoparticles for photo-thermal therapy in a 3d bioprinted glioblastoma tumor model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9455633/
https://www.ncbi.nlm.nih.gov/pubmed/36076927
http://dx.doi.org/10.3390/ijms23179528
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