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PCR Mediated Nucleic Acid Molecular Recognition Technology for Detection of Viable and Dead Foodborne Pathogens

Living foodborne pathogens pose a serious threat to public and population health. To ensure food safety, it is necessary to complete the detection of viable bacteria in a short time (several hours to 1 day). However, the traditional methods by bacterial culture, as the gold standard, are cumbersome...

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Autores principales: Chen, Mengtao, Lan, Xinyue, Zhu, Longjiao, Ru, Ping, Xu, Wentao, Liu, Haiyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9455676/
https://www.ncbi.nlm.nih.gov/pubmed/36076861
http://dx.doi.org/10.3390/foods11172675
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author Chen, Mengtao
Lan, Xinyue
Zhu, Longjiao
Ru, Ping
Xu, Wentao
Liu, Haiyan
author_facet Chen, Mengtao
Lan, Xinyue
Zhu, Longjiao
Ru, Ping
Xu, Wentao
Liu, Haiyan
author_sort Chen, Mengtao
collection PubMed
description Living foodborne pathogens pose a serious threat to public and population health. To ensure food safety, it is necessary to complete the detection of viable bacteria in a short time (several hours to 1 day). However, the traditional methods by bacterial culture, as the gold standard, are cumbersome and time-consuming. To break through the resultant research bottleneck, PCR mediated nucleic acid molecular recognition technologies, including RNA-based reverse transcriptase PCR (RT-PCR) and DNA-based viability PCR (vPCR) have been developed in recent years. They not only sensitively amplify detection signals and quickly report detection results, but also distinguish viable and dead bacteria. Therefore, this review introduces these PCR-mediated techniques independent of culture for viable and dead foodborne pathogen detection from the nucleic acid molecular recognition principal level and describes their whole-process applications in food quality supervision, which provides a useful reference for the development of detection of foodborne pathogens in the future.
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spelling pubmed-94556762022-09-09 PCR Mediated Nucleic Acid Molecular Recognition Technology for Detection of Viable and Dead Foodborne Pathogens Chen, Mengtao Lan, Xinyue Zhu, Longjiao Ru, Ping Xu, Wentao Liu, Haiyan Foods Perspective Living foodborne pathogens pose a serious threat to public and population health. To ensure food safety, it is necessary to complete the detection of viable bacteria in a short time (several hours to 1 day). However, the traditional methods by bacterial culture, as the gold standard, are cumbersome and time-consuming. To break through the resultant research bottleneck, PCR mediated nucleic acid molecular recognition technologies, including RNA-based reverse transcriptase PCR (RT-PCR) and DNA-based viability PCR (vPCR) have been developed in recent years. They not only sensitively amplify detection signals and quickly report detection results, but also distinguish viable and dead bacteria. Therefore, this review introduces these PCR-mediated techniques independent of culture for viable and dead foodborne pathogen detection from the nucleic acid molecular recognition principal level and describes their whole-process applications in food quality supervision, which provides a useful reference for the development of detection of foodborne pathogens in the future. MDPI 2022-09-02 /pmc/articles/PMC9455676/ /pubmed/36076861 http://dx.doi.org/10.3390/foods11172675 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Perspective
Chen, Mengtao
Lan, Xinyue
Zhu, Longjiao
Ru, Ping
Xu, Wentao
Liu, Haiyan
PCR Mediated Nucleic Acid Molecular Recognition Technology for Detection of Viable and Dead Foodborne Pathogens
title PCR Mediated Nucleic Acid Molecular Recognition Technology for Detection of Viable and Dead Foodborne Pathogens
title_full PCR Mediated Nucleic Acid Molecular Recognition Technology for Detection of Viable and Dead Foodborne Pathogens
title_fullStr PCR Mediated Nucleic Acid Molecular Recognition Technology for Detection of Viable and Dead Foodborne Pathogens
title_full_unstemmed PCR Mediated Nucleic Acid Molecular Recognition Technology for Detection of Viable and Dead Foodborne Pathogens
title_short PCR Mediated Nucleic Acid Molecular Recognition Technology for Detection of Viable and Dead Foodborne Pathogens
title_sort pcr mediated nucleic acid molecular recognition technology for detection of viable and dead foodborne pathogens
topic Perspective
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9455676/
https://www.ncbi.nlm.nih.gov/pubmed/36076861
http://dx.doi.org/10.3390/foods11172675
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