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Topographical Distribution and Phenotype of Resident Meibomian Gland Orifice Immune Cells (MOICs) in Mice and the Effects of Topical Benzalkonium Chloride (BAK)

Meibomian gland orifices (MGOs) are located along the eyelid margin and secrete meibum into the tear film. The profile of resident innate immune cells (ICs) at this site is not well understood. The distribution and phenotype of resident ICs around MGOs in mice was investigated and herein defined as...

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Autores principales: Wu, Ching Yi, Wu, Mengliang, Huang, Xin, Gu, Ben J., Maldonado-Codina, Carole, Morgan, Philip B., Downie, Laura E., Chinnery, Holly R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9455816/
https://www.ncbi.nlm.nih.gov/pubmed/36077001
http://dx.doi.org/10.3390/ijms23179589
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author Wu, Ching Yi
Wu, Mengliang
Huang, Xin
Gu, Ben J.
Maldonado-Codina, Carole
Morgan, Philip B.
Downie, Laura E.
Chinnery, Holly R.
author_facet Wu, Ching Yi
Wu, Mengliang
Huang, Xin
Gu, Ben J.
Maldonado-Codina, Carole
Morgan, Philip B.
Downie, Laura E.
Chinnery, Holly R.
author_sort Wu, Ching Yi
collection PubMed
description Meibomian gland orifices (MGOs) are located along the eyelid margin and secrete meibum into the tear film. The profile of resident innate immune cells (ICs) at this site is not well understood. The distribution and phenotype of resident ICs around MGOs in mice was investigated and herein defined as MGO-associated immune cells (MOICs). The effect of topical 0.1% benzalkonium chloride (BAK) on MOICs was also assessed. Eyelids from healthy CD11c(eYFP) and Cx3cr1(gfp/gfp) mice aged three or seven months were compared. ICs were identified as CD11c(+), Cx3cr1(+), and MHC-II(+) using four-colour immunostaining and confocal microscopy. MOIC density was variable but clustered around MGOs. There were more CD11c(+) MOICs in three-month-old compared with seven-month-old mice (three-month-old: 893 [Formula: see text] 449 cells/mm(2) vs. seven-month-old: 593 [Formula: see text] 493 cells/mm(2), p = 0.004). Along the eyelid margin, there was a decreasing gradient of CD11c(+) MOIC density in three-month-old mice (nasal: 1003 [Formula: see text] 369 cells/mm(2), vs. central: 946 [Formula: see text] 574 cells/mm(2), vs. temporal: 731 [Formula: see text] 353 cells/mm(2), p = 0.044). Cx3cr1-deficient mice had two-fold fewer MHC-II(+) MOICs, suggesting a role for Cx3cr1 receptor signaling in meibomian gland surveillance. CD11c(+) MOIC density was lower in BAK-exposed eyes compared to saline-treated controls, suggesting a change in homeostasis. This study provides novel insight into resident ICs located at MGOs, and their contribution to MG homeostasis.
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spelling pubmed-94558162022-09-09 Topographical Distribution and Phenotype of Resident Meibomian Gland Orifice Immune Cells (MOICs) in Mice and the Effects of Topical Benzalkonium Chloride (BAK) Wu, Ching Yi Wu, Mengliang Huang, Xin Gu, Ben J. Maldonado-Codina, Carole Morgan, Philip B. Downie, Laura E. Chinnery, Holly R. Int J Mol Sci Article Meibomian gland orifices (MGOs) are located along the eyelid margin and secrete meibum into the tear film. The profile of resident innate immune cells (ICs) at this site is not well understood. The distribution and phenotype of resident ICs around MGOs in mice was investigated and herein defined as MGO-associated immune cells (MOICs). The effect of topical 0.1% benzalkonium chloride (BAK) on MOICs was also assessed. Eyelids from healthy CD11c(eYFP) and Cx3cr1(gfp/gfp) mice aged three or seven months were compared. ICs were identified as CD11c(+), Cx3cr1(+), and MHC-II(+) using four-colour immunostaining and confocal microscopy. MOIC density was variable but clustered around MGOs. There were more CD11c(+) MOICs in three-month-old compared with seven-month-old mice (three-month-old: 893 [Formula: see text] 449 cells/mm(2) vs. seven-month-old: 593 [Formula: see text] 493 cells/mm(2), p = 0.004). Along the eyelid margin, there was a decreasing gradient of CD11c(+) MOIC density in three-month-old mice (nasal: 1003 [Formula: see text] 369 cells/mm(2), vs. central: 946 [Formula: see text] 574 cells/mm(2), vs. temporal: 731 [Formula: see text] 353 cells/mm(2), p = 0.044). Cx3cr1-deficient mice had two-fold fewer MHC-II(+) MOICs, suggesting a role for Cx3cr1 receptor signaling in meibomian gland surveillance. CD11c(+) MOIC density was lower in BAK-exposed eyes compared to saline-treated controls, suggesting a change in homeostasis. This study provides novel insight into resident ICs located at MGOs, and their contribution to MG homeostasis. MDPI 2022-08-24 /pmc/articles/PMC9455816/ /pubmed/36077001 http://dx.doi.org/10.3390/ijms23179589 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wu, Ching Yi
Wu, Mengliang
Huang, Xin
Gu, Ben J.
Maldonado-Codina, Carole
Morgan, Philip B.
Downie, Laura E.
Chinnery, Holly R.
Topographical Distribution and Phenotype of Resident Meibomian Gland Orifice Immune Cells (MOICs) in Mice and the Effects of Topical Benzalkonium Chloride (BAK)
title Topographical Distribution and Phenotype of Resident Meibomian Gland Orifice Immune Cells (MOICs) in Mice and the Effects of Topical Benzalkonium Chloride (BAK)
title_full Topographical Distribution and Phenotype of Resident Meibomian Gland Orifice Immune Cells (MOICs) in Mice and the Effects of Topical Benzalkonium Chloride (BAK)
title_fullStr Topographical Distribution and Phenotype of Resident Meibomian Gland Orifice Immune Cells (MOICs) in Mice and the Effects of Topical Benzalkonium Chloride (BAK)
title_full_unstemmed Topographical Distribution and Phenotype of Resident Meibomian Gland Orifice Immune Cells (MOICs) in Mice and the Effects of Topical Benzalkonium Chloride (BAK)
title_short Topographical Distribution and Phenotype of Resident Meibomian Gland Orifice Immune Cells (MOICs) in Mice and the Effects of Topical Benzalkonium Chloride (BAK)
title_sort topographical distribution and phenotype of resident meibomian gland orifice immune cells (moics) in mice and the effects of topical benzalkonium chloride (bak)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9455816/
https://www.ncbi.nlm.nih.gov/pubmed/36077001
http://dx.doi.org/10.3390/ijms23179589
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