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FBB18 participates in preassembly of almost all axonemal dyneins independent of R2TP complex
Assembly of dynein arms requires cytoplasmic processes which are mediated by dynein preassembly factors (DNAAFs). CFAP298, which is conserved in organisms with motile cilia, is required for assembly of dynein arms but with obscure mechanisms. Here, we show that FBB18, a Chlamydomonas homologue of CF...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9455862/ https://www.ncbi.nlm.nih.gov/pubmed/36026524 http://dx.doi.org/10.1371/journal.pgen.1010374 |
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author | Wang, Limei Li, Xuecheng Liu, Guang Pan, Junmin |
author_facet | Wang, Limei Li, Xuecheng Liu, Guang Pan, Junmin |
author_sort | Wang, Limei |
collection | PubMed |
description | Assembly of dynein arms requires cytoplasmic processes which are mediated by dynein preassembly factors (DNAAFs). CFAP298, which is conserved in organisms with motile cilia, is required for assembly of dynein arms but with obscure mechanisms. Here, we show that FBB18, a Chlamydomonas homologue of CFAP298, localizes to the cytoplasm and functions in folding/stabilization of almost all axonemal dyneins at the early steps of dynein preassembly. Mutation of FBB18 causes no or short cilia accompanied with partial loss of both outer and inner dynein arms. Comparative proteomics using (15)N labeling suggests partial degradation of almost all axonemal dynein heavy chains (DHCs). A mutant mimicking a patient variant induces particular loss of DHCα. FBB18 associates with 9 DNAAFs and 14 out of 15 dynein HCs but not with IC1/IC2. FBB18 interacts with RuvBL1/2, components of the HSP90 co-chaperone R2TP complex but not the holo-R2TP complex. Further analysis suggests simultaneous formation of multiple DNAAF complexes involves dynein folding/stability and thus provides new insights into axonemal dynein preassembly. |
format | Online Article Text |
id | pubmed-9455862 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-94558622022-09-09 FBB18 participates in preassembly of almost all axonemal dyneins independent of R2TP complex Wang, Limei Li, Xuecheng Liu, Guang Pan, Junmin PLoS Genet Research Article Assembly of dynein arms requires cytoplasmic processes which are mediated by dynein preassembly factors (DNAAFs). CFAP298, which is conserved in organisms with motile cilia, is required for assembly of dynein arms but with obscure mechanisms. Here, we show that FBB18, a Chlamydomonas homologue of CFAP298, localizes to the cytoplasm and functions in folding/stabilization of almost all axonemal dyneins at the early steps of dynein preassembly. Mutation of FBB18 causes no or short cilia accompanied with partial loss of both outer and inner dynein arms. Comparative proteomics using (15)N labeling suggests partial degradation of almost all axonemal dynein heavy chains (DHCs). A mutant mimicking a patient variant induces particular loss of DHCα. FBB18 associates with 9 DNAAFs and 14 out of 15 dynein HCs but not with IC1/IC2. FBB18 interacts with RuvBL1/2, components of the HSP90 co-chaperone R2TP complex but not the holo-R2TP complex. Further analysis suggests simultaneous formation of multiple DNAAF complexes involves dynein folding/stability and thus provides new insights into axonemal dynein preassembly. Public Library of Science 2022-08-26 /pmc/articles/PMC9455862/ /pubmed/36026524 http://dx.doi.org/10.1371/journal.pgen.1010374 Text en © 2022 Wang et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Wang, Limei Li, Xuecheng Liu, Guang Pan, Junmin FBB18 participates in preassembly of almost all axonemal dyneins independent of R2TP complex |
title | FBB18 participates in preassembly of almost all axonemal dyneins independent of R2TP complex |
title_full | FBB18 participates in preassembly of almost all axonemal dyneins independent of R2TP complex |
title_fullStr | FBB18 participates in preassembly of almost all axonemal dyneins independent of R2TP complex |
title_full_unstemmed | FBB18 participates in preassembly of almost all axonemal dyneins independent of R2TP complex |
title_short | FBB18 participates in preassembly of almost all axonemal dyneins independent of R2TP complex |
title_sort | fbb18 participates in preassembly of almost all axonemal dyneins independent of r2tp complex |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9455862/ https://www.ncbi.nlm.nih.gov/pubmed/36026524 http://dx.doi.org/10.1371/journal.pgen.1010374 |
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