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Anti-Inflammatory Action of Dexmedetomidine on Human Microglial Cells

Neuroinflammation, where inflammatory cytokines are produced in excess, contributes to the pathogenesis of delirium. Microglial cells play a central role in neuroinflammation by producing and releasing inflammatory cytokines in response to infection, tissue damage and neurodegeneration. Dexmedetomid...

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Autores principales: Yamazaki, Sho, Yamaguchi, Keisuke, Someya, Akimasa, Nagaoka, Isao, Hayashida, Masakazu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9455981/
https://www.ncbi.nlm.nih.gov/pubmed/36077505
http://dx.doi.org/10.3390/ijms231710096
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author Yamazaki, Sho
Yamaguchi, Keisuke
Someya, Akimasa
Nagaoka, Isao
Hayashida, Masakazu
author_facet Yamazaki, Sho
Yamaguchi, Keisuke
Someya, Akimasa
Nagaoka, Isao
Hayashida, Masakazu
author_sort Yamazaki, Sho
collection PubMed
description Neuroinflammation, where inflammatory cytokines are produced in excess, contributes to the pathogenesis of delirium. Microglial cells play a central role in neuroinflammation by producing and releasing inflammatory cytokines in response to infection, tissue damage and neurodegeneration. Dexmedetomidine (DEX) is a sedative, which reduces the incidence of delirium. Thus, we hypothesized that DEX may alleviate delirium by exhibiting anti-inflammatory action on microglia. In the present study, we investigated the anti-inflammatory action of DEX on human microglial HMC3 cells. The results indicated that DEX partially suppressed the IL-6 and IL-8 production by lipopolysaccharide (LPS)-stimulated HMC3 cells as well as the phosphorylation of p38 MAPK and IκB and the translocation of NF-κB. Furthermore, DEX substantially suppressed IL-6 and IL-8 production by unstimulated HMC3 cells as wells as the phosphorylation of p38 MAPK and IκB and the translocation of NF-κB. These observations suggest that DEX exhibits anti-inflammatory action on not only LPS-stimulated but also unstimulated microglial cells via the suppression of inflammatory signaling and cytokine production.
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spelling pubmed-94559812022-09-09 Anti-Inflammatory Action of Dexmedetomidine on Human Microglial Cells Yamazaki, Sho Yamaguchi, Keisuke Someya, Akimasa Nagaoka, Isao Hayashida, Masakazu Int J Mol Sci Article Neuroinflammation, where inflammatory cytokines are produced in excess, contributes to the pathogenesis of delirium. Microglial cells play a central role in neuroinflammation by producing and releasing inflammatory cytokines in response to infection, tissue damage and neurodegeneration. Dexmedetomidine (DEX) is a sedative, which reduces the incidence of delirium. Thus, we hypothesized that DEX may alleviate delirium by exhibiting anti-inflammatory action on microglia. In the present study, we investigated the anti-inflammatory action of DEX on human microglial HMC3 cells. The results indicated that DEX partially suppressed the IL-6 and IL-8 production by lipopolysaccharide (LPS)-stimulated HMC3 cells as well as the phosphorylation of p38 MAPK and IκB and the translocation of NF-κB. Furthermore, DEX substantially suppressed IL-6 and IL-8 production by unstimulated HMC3 cells as wells as the phosphorylation of p38 MAPK and IκB and the translocation of NF-κB. These observations suggest that DEX exhibits anti-inflammatory action on not only LPS-stimulated but also unstimulated microglial cells via the suppression of inflammatory signaling and cytokine production. MDPI 2022-09-03 /pmc/articles/PMC9455981/ /pubmed/36077505 http://dx.doi.org/10.3390/ijms231710096 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yamazaki, Sho
Yamaguchi, Keisuke
Someya, Akimasa
Nagaoka, Isao
Hayashida, Masakazu
Anti-Inflammatory Action of Dexmedetomidine on Human Microglial Cells
title Anti-Inflammatory Action of Dexmedetomidine on Human Microglial Cells
title_full Anti-Inflammatory Action of Dexmedetomidine on Human Microglial Cells
title_fullStr Anti-Inflammatory Action of Dexmedetomidine on Human Microglial Cells
title_full_unstemmed Anti-Inflammatory Action of Dexmedetomidine on Human Microglial Cells
title_short Anti-Inflammatory Action of Dexmedetomidine on Human Microglial Cells
title_sort anti-inflammatory action of dexmedetomidine on human microglial cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9455981/
https://www.ncbi.nlm.nih.gov/pubmed/36077505
http://dx.doi.org/10.3390/ijms231710096
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