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Anti-Inflammatory Action of Dexmedetomidine on Human Microglial Cells
Neuroinflammation, where inflammatory cytokines are produced in excess, contributes to the pathogenesis of delirium. Microglial cells play a central role in neuroinflammation by producing and releasing inflammatory cytokines in response to infection, tissue damage and neurodegeneration. Dexmedetomid...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9455981/ https://www.ncbi.nlm.nih.gov/pubmed/36077505 http://dx.doi.org/10.3390/ijms231710096 |
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author | Yamazaki, Sho Yamaguchi, Keisuke Someya, Akimasa Nagaoka, Isao Hayashida, Masakazu |
author_facet | Yamazaki, Sho Yamaguchi, Keisuke Someya, Akimasa Nagaoka, Isao Hayashida, Masakazu |
author_sort | Yamazaki, Sho |
collection | PubMed |
description | Neuroinflammation, where inflammatory cytokines are produced in excess, contributes to the pathogenesis of delirium. Microglial cells play a central role in neuroinflammation by producing and releasing inflammatory cytokines in response to infection, tissue damage and neurodegeneration. Dexmedetomidine (DEX) is a sedative, which reduces the incidence of delirium. Thus, we hypothesized that DEX may alleviate delirium by exhibiting anti-inflammatory action on microglia. In the present study, we investigated the anti-inflammatory action of DEX on human microglial HMC3 cells. The results indicated that DEX partially suppressed the IL-6 and IL-8 production by lipopolysaccharide (LPS)-stimulated HMC3 cells as well as the phosphorylation of p38 MAPK and IκB and the translocation of NF-κB. Furthermore, DEX substantially suppressed IL-6 and IL-8 production by unstimulated HMC3 cells as wells as the phosphorylation of p38 MAPK and IκB and the translocation of NF-κB. These observations suggest that DEX exhibits anti-inflammatory action on not only LPS-stimulated but also unstimulated microglial cells via the suppression of inflammatory signaling and cytokine production. |
format | Online Article Text |
id | pubmed-9455981 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94559812022-09-09 Anti-Inflammatory Action of Dexmedetomidine on Human Microglial Cells Yamazaki, Sho Yamaguchi, Keisuke Someya, Akimasa Nagaoka, Isao Hayashida, Masakazu Int J Mol Sci Article Neuroinflammation, where inflammatory cytokines are produced in excess, contributes to the pathogenesis of delirium. Microglial cells play a central role in neuroinflammation by producing and releasing inflammatory cytokines in response to infection, tissue damage and neurodegeneration. Dexmedetomidine (DEX) is a sedative, which reduces the incidence of delirium. Thus, we hypothesized that DEX may alleviate delirium by exhibiting anti-inflammatory action on microglia. In the present study, we investigated the anti-inflammatory action of DEX on human microglial HMC3 cells. The results indicated that DEX partially suppressed the IL-6 and IL-8 production by lipopolysaccharide (LPS)-stimulated HMC3 cells as well as the phosphorylation of p38 MAPK and IκB and the translocation of NF-κB. Furthermore, DEX substantially suppressed IL-6 and IL-8 production by unstimulated HMC3 cells as wells as the phosphorylation of p38 MAPK and IκB and the translocation of NF-κB. These observations suggest that DEX exhibits anti-inflammatory action on not only LPS-stimulated but also unstimulated microglial cells via the suppression of inflammatory signaling and cytokine production. MDPI 2022-09-03 /pmc/articles/PMC9455981/ /pubmed/36077505 http://dx.doi.org/10.3390/ijms231710096 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yamazaki, Sho Yamaguchi, Keisuke Someya, Akimasa Nagaoka, Isao Hayashida, Masakazu Anti-Inflammatory Action of Dexmedetomidine on Human Microglial Cells |
title | Anti-Inflammatory Action of Dexmedetomidine on Human Microglial Cells |
title_full | Anti-Inflammatory Action of Dexmedetomidine on Human Microglial Cells |
title_fullStr | Anti-Inflammatory Action of Dexmedetomidine on Human Microglial Cells |
title_full_unstemmed | Anti-Inflammatory Action of Dexmedetomidine on Human Microglial Cells |
title_short | Anti-Inflammatory Action of Dexmedetomidine on Human Microglial Cells |
title_sort | anti-inflammatory action of dexmedetomidine on human microglial cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9455981/ https://www.ncbi.nlm.nih.gov/pubmed/36077505 http://dx.doi.org/10.3390/ijms231710096 |
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