A New Neutralization Epitope in the Spike Protein of Porcine Epidemic Diarrhea Virus

Porcine epidemic diarrhea virus (PEDV) infects piglets and causes serious diarrhea as well as vomiting, dehydration, and death. The trimeric S protein plays a crucial role in the induction of neutralizing antibodies, and many neutralizing monoclonal antibodies (mAbs) against PEDV S protein have been...

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Autores principales: Liu, Jianbo, Shi, Hongyan, Chen, Jianfei, Zhang, Xin, Shi, Da, Ji, Zhaoyang, Jing, Zhaoyang, Feng, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456025/
https://www.ncbi.nlm.nih.gov/pubmed/36077070
http://dx.doi.org/10.3390/ijms23179674
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author Liu, Jianbo
Shi, Hongyan
Chen, Jianfei
Zhang, Xin
Shi, Da
Ji, Zhaoyang
Jing, Zhaoyang
Feng, Li
author_facet Liu, Jianbo
Shi, Hongyan
Chen, Jianfei
Zhang, Xin
Shi, Da
Ji, Zhaoyang
Jing, Zhaoyang
Feng, Li
author_sort Liu, Jianbo
collection PubMed
description Porcine epidemic diarrhea virus (PEDV) infects piglets and causes serious diarrhea as well as vomiting, dehydration, and death. The trimeric S protein plays a crucial role in the induction of neutralizing antibodies, and many neutralizing monoclonal antibodies (mAbs) against PEDV S protein have been developed. However, these mAbs exclusively target the S1 protein. In this study, we obtained a neutralizing mAb, 5F7, against the S2 protein of PEDV, and this mAb could neutralize new variant genotype 2 PEDV strains (LNCT2), as well as a genotype 1 PEDV strain (CV777), in vitro. The core sequence of the epitope was found in amino acid sequence 1261 aa~1337 aa. These findings confirm that the S2 protein possessed neutralizing epitopes and provided knowledge to aid further research on this virus.
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spelling pubmed-94560252022-09-09 A New Neutralization Epitope in the Spike Protein of Porcine Epidemic Diarrhea Virus Liu, Jianbo Shi, Hongyan Chen, Jianfei Zhang, Xin Shi, Da Ji, Zhaoyang Jing, Zhaoyang Feng, Li Int J Mol Sci Article Porcine epidemic diarrhea virus (PEDV) infects piglets and causes serious diarrhea as well as vomiting, dehydration, and death. The trimeric S protein plays a crucial role in the induction of neutralizing antibodies, and many neutralizing monoclonal antibodies (mAbs) against PEDV S protein have been developed. However, these mAbs exclusively target the S1 protein. In this study, we obtained a neutralizing mAb, 5F7, against the S2 protein of PEDV, and this mAb could neutralize new variant genotype 2 PEDV strains (LNCT2), as well as a genotype 1 PEDV strain (CV777), in vitro. The core sequence of the epitope was found in amino acid sequence 1261 aa~1337 aa. These findings confirm that the S2 protein possessed neutralizing epitopes and provided knowledge to aid further research on this virus. MDPI 2022-08-26 /pmc/articles/PMC9456025/ /pubmed/36077070 http://dx.doi.org/10.3390/ijms23179674 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Jianbo
Shi, Hongyan
Chen, Jianfei
Zhang, Xin
Shi, Da
Ji, Zhaoyang
Jing, Zhaoyang
Feng, Li
A New Neutralization Epitope in the Spike Protein of Porcine Epidemic Diarrhea Virus
title A New Neutralization Epitope in the Spike Protein of Porcine Epidemic Diarrhea Virus
title_full A New Neutralization Epitope in the Spike Protein of Porcine Epidemic Diarrhea Virus
title_fullStr A New Neutralization Epitope in the Spike Protein of Porcine Epidemic Diarrhea Virus
title_full_unstemmed A New Neutralization Epitope in the Spike Protein of Porcine Epidemic Diarrhea Virus
title_short A New Neutralization Epitope in the Spike Protein of Porcine Epidemic Diarrhea Virus
title_sort new neutralization epitope in the spike protein of porcine epidemic diarrhea virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456025/
https://www.ncbi.nlm.nih.gov/pubmed/36077070
http://dx.doi.org/10.3390/ijms23179674
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