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Evaluation of the Effect of the Fibroblast Growth Factor Type 2 (FGF-2) Administration on Placental Gene Expression in a Murine Model of Preeclampsia Induced by L-NAME

The abnormal implantation of the trophoblast during the first trimester of pregnancy precedes the appearance of the clinical manifestations of preeclampsia (PE), which is a hypertensive disorder of pregnancy. In a previous study, which was carried out in a murine model of PE that was induced by NG-n...

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Autores principales: Martinez-Fierro, Margarita L, Garza-Veloz, Idalia, Castañeda-Lopez, Maria Eugenia, Wasike, Dorothy, Castruita-De la Rosa, Claudia, Rodriguez-Sanchez, Iram Pablo, Delgado-Enciso, Ivan, Flores-Mendoza, Jose
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456139/
https://www.ncbi.nlm.nih.gov/pubmed/36077527
http://dx.doi.org/10.3390/ijms231710129
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author Martinez-Fierro, Margarita L
Garza-Veloz, Idalia
Castañeda-Lopez, Maria Eugenia
Wasike, Dorothy
Castruita-De la Rosa, Claudia
Rodriguez-Sanchez, Iram Pablo
Delgado-Enciso, Ivan
Flores-Mendoza, Jose
author_facet Martinez-Fierro, Margarita L
Garza-Veloz, Idalia
Castañeda-Lopez, Maria Eugenia
Wasike, Dorothy
Castruita-De la Rosa, Claudia
Rodriguez-Sanchez, Iram Pablo
Delgado-Enciso, Ivan
Flores-Mendoza, Jose
author_sort Martinez-Fierro, Margarita L
collection PubMed
description The abnormal implantation of the trophoblast during the first trimester of pregnancy precedes the appearance of the clinical manifestations of preeclampsia (PE), which is a hypertensive disorder of pregnancy. In a previous study, which was carried out in a murine model of PE that was induced by NG-nitro-L-arginine methyl ester (L-NAME), we observed that the intravenous administration of fibroblast growth factor 2 (FGF2) had a hypotensive effect, improved the placental weight gain and attenuated the fetal growth restriction, and the morphological findings that were induced by L-NAME in the evaluated tissues were less severe. In this study, we aimed to determine the effect of FGF2 administration on the placental gene expression of the vascular endothelial growth factor (VEGFA), VEGF receptor 2 (VEGFR2), placental growth factor, endoglin (ENG), superoxide dismutase 1 (SOD1), catalase (CAT), thioredoxin (TXN), tumor protein P53 (P53), BCL2 apoptosis regulator, Fas cell surface death receptor (FAS), and caspase 3, in a Sprague Dawley rat PE model, which was induced by L-NAME. The gene expression was determined by a real-time polymerase chain reaction using SYBR green. Taking the vehicle or the L-NAME group as a reference, there was an under expression of placental VEGFA, VEGFR2, ENG, P53, FAS, SOD1, CAT, and TXN genes in the group of L-NAME + FGF2 (p < 0.05). The administration of FGF2 in the murine PE-like model that was induced by L-NAME reduced the effects that were generated by proteinuria and the increased BP, as well as the response of the expression of genes that participate in angiogenesis, apoptosis, and OS. These results have generated valuable information regarding the identification of molecular targets for PE and provide new insights for understanding PE pathogenesis.
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spelling pubmed-94561392022-09-09 Evaluation of the Effect of the Fibroblast Growth Factor Type 2 (FGF-2) Administration on Placental Gene Expression in a Murine Model of Preeclampsia Induced by L-NAME Martinez-Fierro, Margarita L Garza-Veloz, Idalia Castañeda-Lopez, Maria Eugenia Wasike, Dorothy Castruita-De la Rosa, Claudia Rodriguez-Sanchez, Iram Pablo Delgado-Enciso, Ivan Flores-Mendoza, Jose Int J Mol Sci Article The abnormal implantation of the trophoblast during the first trimester of pregnancy precedes the appearance of the clinical manifestations of preeclampsia (PE), which is a hypertensive disorder of pregnancy. In a previous study, which was carried out in a murine model of PE that was induced by NG-nitro-L-arginine methyl ester (L-NAME), we observed that the intravenous administration of fibroblast growth factor 2 (FGF2) had a hypotensive effect, improved the placental weight gain and attenuated the fetal growth restriction, and the morphological findings that were induced by L-NAME in the evaluated tissues were less severe. In this study, we aimed to determine the effect of FGF2 administration on the placental gene expression of the vascular endothelial growth factor (VEGFA), VEGF receptor 2 (VEGFR2), placental growth factor, endoglin (ENG), superoxide dismutase 1 (SOD1), catalase (CAT), thioredoxin (TXN), tumor protein P53 (P53), BCL2 apoptosis regulator, Fas cell surface death receptor (FAS), and caspase 3, in a Sprague Dawley rat PE model, which was induced by L-NAME. The gene expression was determined by a real-time polymerase chain reaction using SYBR green. Taking the vehicle or the L-NAME group as a reference, there was an under expression of placental VEGFA, VEGFR2, ENG, P53, FAS, SOD1, CAT, and TXN genes in the group of L-NAME + FGF2 (p < 0.05). The administration of FGF2 in the murine PE-like model that was induced by L-NAME reduced the effects that were generated by proteinuria and the increased BP, as well as the response of the expression of genes that participate in angiogenesis, apoptosis, and OS. These results have generated valuable information regarding the identification of molecular targets for PE and provide new insights for understanding PE pathogenesis. MDPI 2022-09-04 /pmc/articles/PMC9456139/ /pubmed/36077527 http://dx.doi.org/10.3390/ijms231710129 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Martinez-Fierro, Margarita L
Garza-Veloz, Idalia
Castañeda-Lopez, Maria Eugenia
Wasike, Dorothy
Castruita-De la Rosa, Claudia
Rodriguez-Sanchez, Iram Pablo
Delgado-Enciso, Ivan
Flores-Mendoza, Jose
Evaluation of the Effect of the Fibroblast Growth Factor Type 2 (FGF-2) Administration on Placental Gene Expression in a Murine Model of Preeclampsia Induced by L-NAME
title Evaluation of the Effect of the Fibroblast Growth Factor Type 2 (FGF-2) Administration on Placental Gene Expression in a Murine Model of Preeclampsia Induced by L-NAME
title_full Evaluation of the Effect of the Fibroblast Growth Factor Type 2 (FGF-2) Administration on Placental Gene Expression in a Murine Model of Preeclampsia Induced by L-NAME
title_fullStr Evaluation of the Effect of the Fibroblast Growth Factor Type 2 (FGF-2) Administration on Placental Gene Expression in a Murine Model of Preeclampsia Induced by L-NAME
title_full_unstemmed Evaluation of the Effect of the Fibroblast Growth Factor Type 2 (FGF-2) Administration on Placental Gene Expression in a Murine Model of Preeclampsia Induced by L-NAME
title_short Evaluation of the Effect of the Fibroblast Growth Factor Type 2 (FGF-2) Administration on Placental Gene Expression in a Murine Model of Preeclampsia Induced by L-NAME
title_sort evaluation of the effect of the fibroblast growth factor type 2 (fgf-2) administration on placental gene expression in a murine model of preeclampsia induced by l-name
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456139/
https://www.ncbi.nlm.nih.gov/pubmed/36077527
http://dx.doi.org/10.3390/ijms231710129
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