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A Novel Pathway of Chlorimuron-Ethyl Biodegradation by Chenggangzhangella methanolivorans Strain CHL1 and Its Molecular Mechanisms

Chlorimuron-ethyl is a widely used herbicide in agriculture. However, uncontrolled chlorimuron-ethyl application causes serious environmental problems. Chlorimuron-ethyl can be effectively degraded by microbes, but the underlying molecular mechanisms are not fully understood. In this study, we ident...

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Autores principales: Yu, Zhixiong, Dai, Yumeng, Li, Tingting, Gu, Wu, Yang, Yi, Li, Xiang, Peng, Pai, Yang, Lijie, Li, Xinyu, Wang, Jian, Su, Zhencheng, Li, Xu, Xu, Mingkai, Zhang, Huiwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456165/
https://www.ncbi.nlm.nih.gov/pubmed/36077288
http://dx.doi.org/10.3390/ijms23179890
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author Yu, Zhixiong
Dai, Yumeng
Li, Tingting
Gu, Wu
Yang, Yi
Li, Xiang
Peng, Pai
Yang, Lijie
Li, Xinyu
Wang, Jian
Su, Zhencheng
Li, Xu
Xu, Mingkai
Zhang, Huiwen
author_facet Yu, Zhixiong
Dai, Yumeng
Li, Tingting
Gu, Wu
Yang, Yi
Li, Xiang
Peng, Pai
Yang, Lijie
Li, Xinyu
Wang, Jian
Su, Zhencheng
Li, Xu
Xu, Mingkai
Zhang, Huiwen
author_sort Yu, Zhixiong
collection PubMed
description Chlorimuron-ethyl is a widely used herbicide in agriculture. However, uncontrolled chlorimuron-ethyl application causes serious environmental problems. Chlorimuron-ethyl can be effectively degraded by microbes, but the underlying molecular mechanisms are not fully understood. In this study, we identified the possible pathways and key genes involved in chlorimuron-ethyl degradation by the Chenggangzhangella methanolivorans strain CHL1, a Methylocystaceae strain with the ability to degrade sulfonylurea herbicides. Using a metabolomics method, eight intermediate degradation products were identified, and three pathways, including a novel pyrimidine-ring-opening pathway, were found to be involved in chlorimuron-ethyl degradation by strain CHL1. Transcriptome sequencing indicated that three genes (atzF, atzD, and cysJ) are involved in chlorimuron-ethyl degradation by strain CHL1. The gene knock-out and complementation techniques allowed for the functions of the three genes to be identified, and the enzymes involved in the different steps of chlorimuron-ethyl degradation pathways were preliminary predicted. The results reveal a previously unreported pathway and the key genes of chlorimuron-ethyl degradation by strain CHL1, which have implications for attempts to enrich the biodegradation mechanism of sulfonylurea herbicides and to construct engineered bacteria in order to remove sulfonylurea herbicide residues from environmental media.
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spelling pubmed-94561652022-09-09 A Novel Pathway of Chlorimuron-Ethyl Biodegradation by Chenggangzhangella methanolivorans Strain CHL1 and Its Molecular Mechanisms Yu, Zhixiong Dai, Yumeng Li, Tingting Gu, Wu Yang, Yi Li, Xiang Peng, Pai Yang, Lijie Li, Xinyu Wang, Jian Su, Zhencheng Li, Xu Xu, Mingkai Zhang, Huiwen Int J Mol Sci Article Chlorimuron-ethyl is a widely used herbicide in agriculture. However, uncontrolled chlorimuron-ethyl application causes serious environmental problems. Chlorimuron-ethyl can be effectively degraded by microbes, but the underlying molecular mechanisms are not fully understood. In this study, we identified the possible pathways and key genes involved in chlorimuron-ethyl degradation by the Chenggangzhangella methanolivorans strain CHL1, a Methylocystaceae strain with the ability to degrade sulfonylurea herbicides. Using a metabolomics method, eight intermediate degradation products were identified, and three pathways, including a novel pyrimidine-ring-opening pathway, were found to be involved in chlorimuron-ethyl degradation by strain CHL1. Transcriptome sequencing indicated that three genes (atzF, atzD, and cysJ) are involved in chlorimuron-ethyl degradation by strain CHL1. The gene knock-out and complementation techniques allowed for the functions of the three genes to be identified, and the enzymes involved in the different steps of chlorimuron-ethyl degradation pathways were preliminary predicted. The results reveal a previously unreported pathway and the key genes of chlorimuron-ethyl degradation by strain CHL1, which have implications for attempts to enrich the biodegradation mechanism of sulfonylurea herbicides and to construct engineered bacteria in order to remove sulfonylurea herbicide residues from environmental media. MDPI 2022-08-31 /pmc/articles/PMC9456165/ /pubmed/36077288 http://dx.doi.org/10.3390/ijms23179890 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yu, Zhixiong
Dai, Yumeng
Li, Tingting
Gu, Wu
Yang, Yi
Li, Xiang
Peng, Pai
Yang, Lijie
Li, Xinyu
Wang, Jian
Su, Zhencheng
Li, Xu
Xu, Mingkai
Zhang, Huiwen
A Novel Pathway of Chlorimuron-Ethyl Biodegradation by Chenggangzhangella methanolivorans Strain CHL1 and Its Molecular Mechanisms
title A Novel Pathway of Chlorimuron-Ethyl Biodegradation by Chenggangzhangella methanolivorans Strain CHL1 and Its Molecular Mechanisms
title_full A Novel Pathway of Chlorimuron-Ethyl Biodegradation by Chenggangzhangella methanolivorans Strain CHL1 and Its Molecular Mechanisms
title_fullStr A Novel Pathway of Chlorimuron-Ethyl Biodegradation by Chenggangzhangella methanolivorans Strain CHL1 and Its Molecular Mechanisms
title_full_unstemmed A Novel Pathway of Chlorimuron-Ethyl Biodegradation by Chenggangzhangella methanolivorans Strain CHL1 and Its Molecular Mechanisms
title_short A Novel Pathway of Chlorimuron-Ethyl Biodegradation by Chenggangzhangella methanolivorans Strain CHL1 and Its Molecular Mechanisms
title_sort novel pathway of chlorimuron-ethyl biodegradation by chenggangzhangella methanolivorans strain chl1 and its molecular mechanisms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456165/
https://www.ncbi.nlm.nih.gov/pubmed/36077288
http://dx.doi.org/10.3390/ijms23179890
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