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Telomere Shortening and Increased Oxidative Stress in Lumbar Disc Degeneration
Lumbar disc degeneration (LDD) contributes to low back pain. This study aimed to determine relative telomere length (RTL), oxidative stress status, and antioxidant levels and examine the relationships between RTL, oxidative stress, and the severity in LDD patients. A total of 100 subjects, 50 LDD pa...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456205/ https://www.ncbi.nlm.nih.gov/pubmed/36077523 http://dx.doi.org/10.3390/ijms231710125 |
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author | Jitjumnong, Manassanan Chalermkitpanit, Pornpan Suantawee, Tanyawan Dechsupa, Sinsuda Vajarintarangoon, Ladawan Honsawek, Sittisak |
author_facet | Jitjumnong, Manassanan Chalermkitpanit, Pornpan Suantawee, Tanyawan Dechsupa, Sinsuda Vajarintarangoon, Ladawan Honsawek, Sittisak |
author_sort | Jitjumnong, Manassanan |
collection | PubMed |
description | Lumbar disc degeneration (LDD) contributes to low back pain. This study aimed to determine relative telomere length (RTL), oxidative stress status, and antioxidant levels and examine the relationships between RTL, oxidative stress, and the severity in LDD patients. A total of 100 subjects, 50 LDD patients and 50 healthy controls, were enrolled in the case–control study. Blood leukocyte RTL was analyzed using quantitative real-time polymerase chain reaction. Lipid peroxidation was determined by malondialdehyde (MDA) assay. Plasma 8-hydroxy 2′-deoxyguanosine (8-OHdG) values were determined using enzyme-linked immunosorbent assay. Total antioxidant capacity (TAC) and ferric reducing antioxidant power (FRAP) in plasma were also measured. The LDD patients had significantly shorter telomeres than the healthy controls (p = 0.04). Blood leukocyte RTL was inversely correlated with the LDD severity (r = −0.41, p = 0.005). Additionally, plasma MDA and 8-OHdG levels were markedly greater in LDD patients than in the controls (p = 0.01 and p = 0.002, respectively). Furthermore, the plasma MDA level showed a positive correlation with the radiographic severity (r = 0.49, p = 0.001). There was a positive correlation between plasma 8-OHdG and the severity (r = 0.60, p < 0.001). Moreover, plasma TAC and FRAP levels were significantly lower in LDD patients than in the controls (p = 0.04). No significant differences in plasma TAC and FRAP were observed among the three groups of LDD severity. We found that RTL was negatively correlated with the severity while plasma MDA and 8-OHdG levels were positively correlated with the severity. These findings suggest that blood leukocyte RTL, plasma MDA, and 8-OHdG may have potential as noninvasive biomarkers for the assessment of severity in LDD. |
format | Online Article Text |
id | pubmed-9456205 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94562052022-09-09 Telomere Shortening and Increased Oxidative Stress in Lumbar Disc Degeneration Jitjumnong, Manassanan Chalermkitpanit, Pornpan Suantawee, Tanyawan Dechsupa, Sinsuda Vajarintarangoon, Ladawan Honsawek, Sittisak Int J Mol Sci Article Lumbar disc degeneration (LDD) contributes to low back pain. This study aimed to determine relative telomere length (RTL), oxidative stress status, and antioxidant levels and examine the relationships between RTL, oxidative stress, and the severity in LDD patients. A total of 100 subjects, 50 LDD patients and 50 healthy controls, were enrolled in the case–control study. Blood leukocyte RTL was analyzed using quantitative real-time polymerase chain reaction. Lipid peroxidation was determined by malondialdehyde (MDA) assay. Plasma 8-hydroxy 2′-deoxyguanosine (8-OHdG) values were determined using enzyme-linked immunosorbent assay. Total antioxidant capacity (TAC) and ferric reducing antioxidant power (FRAP) in plasma were also measured. The LDD patients had significantly shorter telomeres than the healthy controls (p = 0.04). Blood leukocyte RTL was inversely correlated with the LDD severity (r = −0.41, p = 0.005). Additionally, plasma MDA and 8-OHdG levels were markedly greater in LDD patients than in the controls (p = 0.01 and p = 0.002, respectively). Furthermore, the plasma MDA level showed a positive correlation with the radiographic severity (r = 0.49, p = 0.001). There was a positive correlation between plasma 8-OHdG and the severity (r = 0.60, p < 0.001). Moreover, plasma TAC and FRAP levels were significantly lower in LDD patients than in the controls (p = 0.04). No significant differences in plasma TAC and FRAP were observed among the three groups of LDD severity. We found that RTL was negatively correlated with the severity while plasma MDA and 8-OHdG levels were positively correlated with the severity. These findings suggest that blood leukocyte RTL, plasma MDA, and 8-OHdG may have potential as noninvasive biomarkers for the assessment of severity in LDD. MDPI 2022-09-04 /pmc/articles/PMC9456205/ /pubmed/36077523 http://dx.doi.org/10.3390/ijms231710125 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Jitjumnong, Manassanan Chalermkitpanit, Pornpan Suantawee, Tanyawan Dechsupa, Sinsuda Vajarintarangoon, Ladawan Honsawek, Sittisak Telomere Shortening and Increased Oxidative Stress in Lumbar Disc Degeneration |
title | Telomere Shortening and Increased Oxidative Stress in Lumbar Disc Degeneration |
title_full | Telomere Shortening and Increased Oxidative Stress in Lumbar Disc Degeneration |
title_fullStr | Telomere Shortening and Increased Oxidative Stress in Lumbar Disc Degeneration |
title_full_unstemmed | Telomere Shortening and Increased Oxidative Stress in Lumbar Disc Degeneration |
title_short | Telomere Shortening and Increased Oxidative Stress in Lumbar Disc Degeneration |
title_sort | telomere shortening and increased oxidative stress in lumbar disc degeneration |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456205/ https://www.ncbi.nlm.nih.gov/pubmed/36077523 http://dx.doi.org/10.3390/ijms231710125 |
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