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Telomere Shortening and Increased Oxidative Stress in Lumbar Disc Degeneration

Lumbar disc degeneration (LDD) contributes to low back pain. This study aimed to determine relative telomere length (RTL), oxidative stress status, and antioxidant levels and examine the relationships between RTL, oxidative stress, and the severity in LDD patients. A total of 100 subjects, 50 LDD pa...

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Autores principales: Jitjumnong, Manassanan, Chalermkitpanit, Pornpan, Suantawee, Tanyawan, Dechsupa, Sinsuda, Vajarintarangoon, Ladawan, Honsawek, Sittisak
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456205/
https://www.ncbi.nlm.nih.gov/pubmed/36077523
http://dx.doi.org/10.3390/ijms231710125
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author Jitjumnong, Manassanan
Chalermkitpanit, Pornpan
Suantawee, Tanyawan
Dechsupa, Sinsuda
Vajarintarangoon, Ladawan
Honsawek, Sittisak
author_facet Jitjumnong, Manassanan
Chalermkitpanit, Pornpan
Suantawee, Tanyawan
Dechsupa, Sinsuda
Vajarintarangoon, Ladawan
Honsawek, Sittisak
author_sort Jitjumnong, Manassanan
collection PubMed
description Lumbar disc degeneration (LDD) contributes to low back pain. This study aimed to determine relative telomere length (RTL), oxidative stress status, and antioxidant levels and examine the relationships between RTL, oxidative stress, and the severity in LDD patients. A total of 100 subjects, 50 LDD patients and 50 healthy controls, were enrolled in the case–control study. Blood leukocyte RTL was analyzed using quantitative real-time polymerase chain reaction. Lipid peroxidation was determined by malondialdehyde (MDA) assay. Plasma 8-hydroxy 2′-deoxyguanosine (8-OHdG) values were determined using enzyme-linked immunosorbent assay. Total antioxidant capacity (TAC) and ferric reducing antioxidant power (FRAP) in plasma were also measured. The LDD patients had significantly shorter telomeres than the healthy controls (p = 0.04). Blood leukocyte RTL was inversely correlated with the LDD severity (r = −0.41, p = 0.005). Additionally, plasma MDA and 8-OHdG levels were markedly greater in LDD patients than in the controls (p = 0.01 and p = 0.002, respectively). Furthermore, the plasma MDA level showed a positive correlation with the radiographic severity (r = 0.49, p = 0.001). There was a positive correlation between plasma 8-OHdG and the severity (r = 0.60, p < 0.001). Moreover, plasma TAC and FRAP levels were significantly lower in LDD patients than in the controls (p = 0.04). No significant differences in plasma TAC and FRAP were observed among the three groups of LDD severity. We found that RTL was negatively correlated with the severity while plasma MDA and 8-OHdG levels were positively correlated with the severity. These findings suggest that blood leukocyte RTL, plasma MDA, and 8-OHdG may have potential as noninvasive biomarkers for the assessment of severity in LDD.
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spelling pubmed-94562052022-09-09 Telomere Shortening and Increased Oxidative Stress in Lumbar Disc Degeneration Jitjumnong, Manassanan Chalermkitpanit, Pornpan Suantawee, Tanyawan Dechsupa, Sinsuda Vajarintarangoon, Ladawan Honsawek, Sittisak Int J Mol Sci Article Lumbar disc degeneration (LDD) contributes to low back pain. This study aimed to determine relative telomere length (RTL), oxidative stress status, and antioxidant levels and examine the relationships between RTL, oxidative stress, and the severity in LDD patients. A total of 100 subjects, 50 LDD patients and 50 healthy controls, were enrolled in the case–control study. Blood leukocyte RTL was analyzed using quantitative real-time polymerase chain reaction. Lipid peroxidation was determined by malondialdehyde (MDA) assay. Plasma 8-hydroxy 2′-deoxyguanosine (8-OHdG) values were determined using enzyme-linked immunosorbent assay. Total antioxidant capacity (TAC) and ferric reducing antioxidant power (FRAP) in plasma were also measured. The LDD patients had significantly shorter telomeres than the healthy controls (p = 0.04). Blood leukocyte RTL was inversely correlated with the LDD severity (r = −0.41, p = 0.005). Additionally, plasma MDA and 8-OHdG levels were markedly greater in LDD patients than in the controls (p = 0.01 and p = 0.002, respectively). Furthermore, the plasma MDA level showed a positive correlation with the radiographic severity (r = 0.49, p = 0.001). There was a positive correlation between plasma 8-OHdG and the severity (r = 0.60, p < 0.001). Moreover, plasma TAC and FRAP levels were significantly lower in LDD patients than in the controls (p = 0.04). No significant differences in plasma TAC and FRAP were observed among the three groups of LDD severity. We found that RTL was negatively correlated with the severity while plasma MDA and 8-OHdG levels were positively correlated with the severity. These findings suggest that blood leukocyte RTL, plasma MDA, and 8-OHdG may have potential as noninvasive biomarkers for the assessment of severity in LDD. MDPI 2022-09-04 /pmc/articles/PMC9456205/ /pubmed/36077523 http://dx.doi.org/10.3390/ijms231710125 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jitjumnong, Manassanan
Chalermkitpanit, Pornpan
Suantawee, Tanyawan
Dechsupa, Sinsuda
Vajarintarangoon, Ladawan
Honsawek, Sittisak
Telomere Shortening and Increased Oxidative Stress in Lumbar Disc Degeneration
title Telomere Shortening and Increased Oxidative Stress in Lumbar Disc Degeneration
title_full Telomere Shortening and Increased Oxidative Stress in Lumbar Disc Degeneration
title_fullStr Telomere Shortening and Increased Oxidative Stress in Lumbar Disc Degeneration
title_full_unstemmed Telomere Shortening and Increased Oxidative Stress in Lumbar Disc Degeneration
title_short Telomere Shortening and Increased Oxidative Stress in Lumbar Disc Degeneration
title_sort telomere shortening and increased oxidative stress in lumbar disc degeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456205/
https://www.ncbi.nlm.nih.gov/pubmed/36077523
http://dx.doi.org/10.3390/ijms231710125
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