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Temporal Expression of Myogenic Regulatory Genes in Different Chicken Breeds during Embryonic Development

The basic units of skeletal muscle in all vertebrates are multinucleate myofibers, which are formed from the fusion of mononuclear myoblasts during the embryonic period. In order to understand the regulation of embryonic muscle development, we selected four chicken breeds, namely, Cornish (CN), Whit...

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Autores principales: Gu, Shuang, Wen, Chaoliang, Li, Junying, Liu, Honghong, Huang, Qiang, Zheng, Jiangxia, Sun, Congjiao, Yang, Ning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456251/
https://www.ncbi.nlm.nih.gov/pubmed/36077516
http://dx.doi.org/10.3390/ijms231710115
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author Gu, Shuang
Wen, Chaoliang
Li, Junying
Liu, Honghong
Huang, Qiang
Zheng, Jiangxia
Sun, Congjiao
Yang, Ning
author_facet Gu, Shuang
Wen, Chaoliang
Li, Junying
Liu, Honghong
Huang, Qiang
Zheng, Jiangxia
Sun, Congjiao
Yang, Ning
author_sort Gu, Shuang
collection PubMed
description The basic units of skeletal muscle in all vertebrates are multinucleate myofibers, which are formed from the fusion of mononuclear myoblasts during the embryonic period. In order to understand the regulation of embryonic muscle development, we selected four chicken breeds, namely, Cornish (CN), White Plymouth Rock (WPR), White Leghorn (WL), and Beijing-You Chicken (BYC), for evaluation of their temporal expression patterns of known key regulatory genes (Myomaker, MYOD, and MSTN) during pectoral muscle (PM) and thigh muscle (TM) development. The highest expression level of Myomaker occurred from embryonic days E13 to E15 for all breeds, indicating that it was the crucial stage of myoblast fusion. Interestingly, the fast-growing CN showed the highest gene expression level of Myomaker during the crucial stage. The MYOD gene expression at D1 was much higher, implying that MYOD might have an important role after hatching. Histomorphology of PM and TM suggested that the myofibers was largely complete at E17, which was speculated to have occurred because of the expression increase in MSTN and the expression decrease in Myomaker. Our research contributes to lay a foundation for the study of myofiber development during the embryonic period in different chicken breeds.
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spelling pubmed-94562512022-09-09 Temporal Expression of Myogenic Regulatory Genes in Different Chicken Breeds during Embryonic Development Gu, Shuang Wen, Chaoliang Li, Junying Liu, Honghong Huang, Qiang Zheng, Jiangxia Sun, Congjiao Yang, Ning Int J Mol Sci Article The basic units of skeletal muscle in all vertebrates are multinucleate myofibers, which are formed from the fusion of mononuclear myoblasts during the embryonic period. In order to understand the regulation of embryonic muscle development, we selected four chicken breeds, namely, Cornish (CN), White Plymouth Rock (WPR), White Leghorn (WL), and Beijing-You Chicken (BYC), for evaluation of their temporal expression patterns of known key regulatory genes (Myomaker, MYOD, and MSTN) during pectoral muscle (PM) and thigh muscle (TM) development. The highest expression level of Myomaker occurred from embryonic days E13 to E15 for all breeds, indicating that it was the crucial stage of myoblast fusion. Interestingly, the fast-growing CN showed the highest gene expression level of Myomaker during the crucial stage. The MYOD gene expression at D1 was much higher, implying that MYOD might have an important role after hatching. Histomorphology of PM and TM suggested that the myofibers was largely complete at E17, which was speculated to have occurred because of the expression increase in MSTN and the expression decrease in Myomaker. Our research contributes to lay a foundation for the study of myofiber development during the embryonic period in different chicken breeds. MDPI 2022-09-04 /pmc/articles/PMC9456251/ /pubmed/36077516 http://dx.doi.org/10.3390/ijms231710115 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gu, Shuang
Wen, Chaoliang
Li, Junying
Liu, Honghong
Huang, Qiang
Zheng, Jiangxia
Sun, Congjiao
Yang, Ning
Temporal Expression of Myogenic Regulatory Genes in Different Chicken Breeds during Embryonic Development
title Temporal Expression of Myogenic Regulatory Genes in Different Chicken Breeds during Embryonic Development
title_full Temporal Expression of Myogenic Regulatory Genes in Different Chicken Breeds during Embryonic Development
title_fullStr Temporal Expression of Myogenic Regulatory Genes in Different Chicken Breeds during Embryonic Development
title_full_unstemmed Temporal Expression of Myogenic Regulatory Genes in Different Chicken Breeds during Embryonic Development
title_short Temporal Expression of Myogenic Regulatory Genes in Different Chicken Breeds during Embryonic Development
title_sort temporal expression of myogenic regulatory genes in different chicken breeds during embryonic development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456251/
https://www.ncbi.nlm.nih.gov/pubmed/36077516
http://dx.doi.org/10.3390/ijms231710115
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