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Subgingival Microbiome in Rheumatoid Arthritis Patients with Periodontitis

Rheumatoid arthritis (RA) and periodontitis are suggested to be closely linked based on microbial dysbiosis, but limited subgingival bacteria have been proven in the pathogenesis of RA. We enrolled 30 RA patients and 25 controls and divided them into three groups with matched age, gender, and diabet...

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Autores principales: Chen, Yi-Jing, Hung, Wei-Chun, Chou, Yu-Hsiang, Lai, Chern-Hsiung, Peng, Po, Jhou, Pei-Syuan, Tsai, Min-Ru, Sheu, Jim Jinn-Chyuan, Yen, Jeng-Hsien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456296/
https://www.ncbi.nlm.nih.gov/pubmed/36077282
http://dx.doi.org/10.3390/ijms23179883
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author Chen, Yi-Jing
Hung, Wei-Chun
Chou, Yu-Hsiang
Lai, Chern-Hsiung
Peng, Po
Jhou, Pei-Syuan
Tsai, Min-Ru
Sheu, Jim Jinn-Chyuan
Yen, Jeng-Hsien
author_facet Chen, Yi-Jing
Hung, Wei-Chun
Chou, Yu-Hsiang
Lai, Chern-Hsiung
Peng, Po
Jhou, Pei-Syuan
Tsai, Min-Ru
Sheu, Jim Jinn-Chyuan
Yen, Jeng-Hsien
author_sort Chen, Yi-Jing
collection PubMed
description Rheumatoid arthritis (RA) and periodontitis are suggested to be closely linked based on microbial dysbiosis, but limited subgingival bacteria have been proven in the pathogenesis of RA. We enrolled 30 RA patients and 25 controls and divided them into three groups with matched age, gender, and diabetes statuses: group AM (all of the matched participants), group PD (periodontally diseased), and group PH (periodontally healthy). Their subgingival microbial composition was determined by V3–V4 16S rRNA gene sequencing. Significant differences in subgingival microbial clustering between the RA patients and controls were observed in groups AM and PD. Among the taxa enriched in RA, Aminipila butyrica and Peptococcus simiae were the only two species displaying positive correlation to the level of anti-citrullinated protein antibodies (ACPAs) in both of the groups. Surprisingly, the median of relative abundances of A. butyrica and P. simiae were 0% in the controls of group PD. Furthermore, a gene encoding arginine deiminase with the capability to produce citrulline was addressed in the complete genome sequence of A. butyrica. This is the first study to elucidate the important roles of A. butyrica and P. simiae as periodontal bacteria leading to RA possibly through the induction of ACPA production.
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spelling pubmed-94562962022-09-09 Subgingival Microbiome in Rheumatoid Arthritis Patients with Periodontitis Chen, Yi-Jing Hung, Wei-Chun Chou, Yu-Hsiang Lai, Chern-Hsiung Peng, Po Jhou, Pei-Syuan Tsai, Min-Ru Sheu, Jim Jinn-Chyuan Yen, Jeng-Hsien Int J Mol Sci Article Rheumatoid arthritis (RA) and periodontitis are suggested to be closely linked based on microbial dysbiosis, but limited subgingival bacteria have been proven in the pathogenesis of RA. We enrolled 30 RA patients and 25 controls and divided them into three groups with matched age, gender, and diabetes statuses: group AM (all of the matched participants), group PD (periodontally diseased), and group PH (periodontally healthy). Their subgingival microbial composition was determined by V3–V4 16S rRNA gene sequencing. Significant differences in subgingival microbial clustering between the RA patients and controls were observed in groups AM and PD. Among the taxa enriched in RA, Aminipila butyrica and Peptococcus simiae were the only two species displaying positive correlation to the level of anti-citrullinated protein antibodies (ACPAs) in both of the groups. Surprisingly, the median of relative abundances of A. butyrica and P. simiae were 0% in the controls of group PD. Furthermore, a gene encoding arginine deiminase with the capability to produce citrulline was addressed in the complete genome sequence of A. butyrica. This is the first study to elucidate the important roles of A. butyrica and P. simiae as periodontal bacteria leading to RA possibly through the induction of ACPA production. MDPI 2022-08-31 /pmc/articles/PMC9456296/ /pubmed/36077282 http://dx.doi.org/10.3390/ijms23179883 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Yi-Jing
Hung, Wei-Chun
Chou, Yu-Hsiang
Lai, Chern-Hsiung
Peng, Po
Jhou, Pei-Syuan
Tsai, Min-Ru
Sheu, Jim Jinn-Chyuan
Yen, Jeng-Hsien
Subgingival Microbiome in Rheumatoid Arthritis Patients with Periodontitis
title Subgingival Microbiome in Rheumatoid Arthritis Patients with Periodontitis
title_full Subgingival Microbiome in Rheumatoid Arthritis Patients with Periodontitis
title_fullStr Subgingival Microbiome in Rheumatoid Arthritis Patients with Periodontitis
title_full_unstemmed Subgingival Microbiome in Rheumatoid Arthritis Patients with Periodontitis
title_short Subgingival Microbiome in Rheumatoid Arthritis Patients with Periodontitis
title_sort subgingival microbiome in rheumatoid arthritis patients with periodontitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456296/
https://www.ncbi.nlm.nih.gov/pubmed/36077282
http://dx.doi.org/10.3390/ijms23179883
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