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Evaluating the Suitability of 3D Bioprinted Samples for Experimental Radiotherapy: A Pilot Study

Radiotherapy is an important component in the treatment of lung cancer, one of the most common cancers worldwide, frequently resulting in death within only a few years of diagnosis. In order to evaluate new therapeutic approaches and compare their efficiency with regard to tumour control at a pre-cl...

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Autores principales: Al-Zeer, Munir A., Prehn, Franziska, Fiedler, Stefan, Lienert, Ulrich, Krisch, Michael, Berg, Johanna, Kurreck, Jens, Hildebrandt, Guido, Schültke, Elisabeth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456381/
https://www.ncbi.nlm.nih.gov/pubmed/36077349
http://dx.doi.org/10.3390/ijms23179951
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author Al-Zeer, Munir A.
Prehn, Franziska
Fiedler, Stefan
Lienert, Ulrich
Krisch, Michael
Berg, Johanna
Kurreck, Jens
Hildebrandt, Guido
Schültke, Elisabeth
author_facet Al-Zeer, Munir A.
Prehn, Franziska
Fiedler, Stefan
Lienert, Ulrich
Krisch, Michael
Berg, Johanna
Kurreck, Jens
Hildebrandt, Guido
Schültke, Elisabeth
author_sort Al-Zeer, Munir A.
collection PubMed
description Radiotherapy is an important component in the treatment of lung cancer, one of the most common cancers worldwide, frequently resulting in death within only a few years of diagnosis. In order to evaluate new therapeutic approaches and compare their efficiency with regard to tumour control at a pre-clinical stage, it is important to develop standardized samples which can serve as inter-institutional outcome controls, independent of differences in local technical parameters or specific techniques. Recent developments in 3D bioprinting techniques could provide a sophisticated solution to this challenge. We have conducted a pilot project to evaluate the suitability of standardized samples generated from 3D printed human lung cancer cells in radiotherapy studies. The samples were irradiated at high dose rates using both broad beam and microbeam techniques. We found the 3D printed constructs to be sufficiently mechanically stable for use in microbeam studies with peak doses up to 400 Gy to test for cytotoxicity, DNA damage, and cancer cell death in vitro. The results of this study show how 3D structures generated from human lung cancer cells in an additive printing process can be used to study the effects of radiotherapy in a standardized manner.
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spelling pubmed-94563812022-09-09 Evaluating the Suitability of 3D Bioprinted Samples for Experimental Radiotherapy: A Pilot Study Al-Zeer, Munir A. Prehn, Franziska Fiedler, Stefan Lienert, Ulrich Krisch, Michael Berg, Johanna Kurreck, Jens Hildebrandt, Guido Schültke, Elisabeth Int J Mol Sci Article Radiotherapy is an important component in the treatment of lung cancer, one of the most common cancers worldwide, frequently resulting in death within only a few years of diagnosis. In order to evaluate new therapeutic approaches and compare their efficiency with regard to tumour control at a pre-clinical stage, it is important to develop standardized samples which can serve as inter-institutional outcome controls, independent of differences in local technical parameters or specific techniques. Recent developments in 3D bioprinting techniques could provide a sophisticated solution to this challenge. We have conducted a pilot project to evaluate the suitability of standardized samples generated from 3D printed human lung cancer cells in radiotherapy studies. The samples were irradiated at high dose rates using both broad beam and microbeam techniques. We found the 3D printed constructs to be sufficiently mechanically stable for use in microbeam studies with peak doses up to 400 Gy to test for cytotoxicity, DNA damage, and cancer cell death in vitro. The results of this study show how 3D structures generated from human lung cancer cells in an additive printing process can be used to study the effects of radiotherapy in a standardized manner. MDPI 2022-09-01 /pmc/articles/PMC9456381/ /pubmed/36077349 http://dx.doi.org/10.3390/ijms23179951 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Al-Zeer, Munir A.
Prehn, Franziska
Fiedler, Stefan
Lienert, Ulrich
Krisch, Michael
Berg, Johanna
Kurreck, Jens
Hildebrandt, Guido
Schültke, Elisabeth
Evaluating the Suitability of 3D Bioprinted Samples for Experimental Radiotherapy: A Pilot Study
title Evaluating the Suitability of 3D Bioprinted Samples for Experimental Radiotherapy: A Pilot Study
title_full Evaluating the Suitability of 3D Bioprinted Samples for Experimental Radiotherapy: A Pilot Study
title_fullStr Evaluating the Suitability of 3D Bioprinted Samples for Experimental Radiotherapy: A Pilot Study
title_full_unstemmed Evaluating the Suitability of 3D Bioprinted Samples for Experimental Radiotherapy: A Pilot Study
title_short Evaluating the Suitability of 3D Bioprinted Samples for Experimental Radiotherapy: A Pilot Study
title_sort evaluating the suitability of 3d bioprinted samples for experimental radiotherapy: a pilot study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456381/
https://www.ncbi.nlm.nih.gov/pubmed/36077349
http://dx.doi.org/10.3390/ijms23179951
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