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Evaluating the Suitability of 3D Bioprinted Samples for Experimental Radiotherapy: A Pilot Study
Radiotherapy is an important component in the treatment of lung cancer, one of the most common cancers worldwide, frequently resulting in death within only a few years of diagnosis. In order to evaluate new therapeutic approaches and compare their efficiency with regard to tumour control at a pre-cl...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456381/ https://www.ncbi.nlm.nih.gov/pubmed/36077349 http://dx.doi.org/10.3390/ijms23179951 |
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author | Al-Zeer, Munir A. Prehn, Franziska Fiedler, Stefan Lienert, Ulrich Krisch, Michael Berg, Johanna Kurreck, Jens Hildebrandt, Guido Schültke, Elisabeth |
author_facet | Al-Zeer, Munir A. Prehn, Franziska Fiedler, Stefan Lienert, Ulrich Krisch, Michael Berg, Johanna Kurreck, Jens Hildebrandt, Guido Schültke, Elisabeth |
author_sort | Al-Zeer, Munir A. |
collection | PubMed |
description | Radiotherapy is an important component in the treatment of lung cancer, one of the most common cancers worldwide, frequently resulting in death within only a few years of diagnosis. In order to evaluate new therapeutic approaches and compare their efficiency with regard to tumour control at a pre-clinical stage, it is important to develop standardized samples which can serve as inter-institutional outcome controls, independent of differences in local technical parameters or specific techniques. Recent developments in 3D bioprinting techniques could provide a sophisticated solution to this challenge. We have conducted a pilot project to evaluate the suitability of standardized samples generated from 3D printed human lung cancer cells in radiotherapy studies. The samples were irradiated at high dose rates using both broad beam and microbeam techniques. We found the 3D printed constructs to be sufficiently mechanically stable for use in microbeam studies with peak doses up to 400 Gy to test for cytotoxicity, DNA damage, and cancer cell death in vitro. The results of this study show how 3D structures generated from human lung cancer cells in an additive printing process can be used to study the effects of radiotherapy in a standardized manner. |
format | Online Article Text |
id | pubmed-9456381 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-94563812022-09-09 Evaluating the Suitability of 3D Bioprinted Samples for Experimental Radiotherapy: A Pilot Study Al-Zeer, Munir A. Prehn, Franziska Fiedler, Stefan Lienert, Ulrich Krisch, Michael Berg, Johanna Kurreck, Jens Hildebrandt, Guido Schültke, Elisabeth Int J Mol Sci Article Radiotherapy is an important component in the treatment of lung cancer, one of the most common cancers worldwide, frequently resulting in death within only a few years of diagnosis. In order to evaluate new therapeutic approaches and compare their efficiency with regard to tumour control at a pre-clinical stage, it is important to develop standardized samples which can serve as inter-institutional outcome controls, independent of differences in local technical parameters or specific techniques. Recent developments in 3D bioprinting techniques could provide a sophisticated solution to this challenge. We have conducted a pilot project to evaluate the suitability of standardized samples generated from 3D printed human lung cancer cells in radiotherapy studies. The samples were irradiated at high dose rates using both broad beam and microbeam techniques. We found the 3D printed constructs to be sufficiently mechanically stable for use in microbeam studies with peak doses up to 400 Gy to test for cytotoxicity, DNA damage, and cancer cell death in vitro. The results of this study show how 3D structures generated from human lung cancer cells in an additive printing process can be used to study the effects of radiotherapy in a standardized manner. MDPI 2022-09-01 /pmc/articles/PMC9456381/ /pubmed/36077349 http://dx.doi.org/10.3390/ijms23179951 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Al-Zeer, Munir A. Prehn, Franziska Fiedler, Stefan Lienert, Ulrich Krisch, Michael Berg, Johanna Kurreck, Jens Hildebrandt, Guido Schültke, Elisabeth Evaluating the Suitability of 3D Bioprinted Samples for Experimental Radiotherapy: A Pilot Study |
title | Evaluating the Suitability of 3D Bioprinted Samples for Experimental Radiotherapy: A Pilot Study |
title_full | Evaluating the Suitability of 3D Bioprinted Samples for Experimental Radiotherapy: A Pilot Study |
title_fullStr | Evaluating the Suitability of 3D Bioprinted Samples for Experimental Radiotherapy: A Pilot Study |
title_full_unstemmed | Evaluating the Suitability of 3D Bioprinted Samples for Experimental Radiotherapy: A Pilot Study |
title_short | Evaluating the Suitability of 3D Bioprinted Samples for Experimental Radiotherapy: A Pilot Study |
title_sort | evaluating the suitability of 3d bioprinted samples for experimental radiotherapy: a pilot study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456381/ https://www.ncbi.nlm.nih.gov/pubmed/36077349 http://dx.doi.org/10.3390/ijms23179951 |
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