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A Bioengineering Approach for the Development of Fibroblast Growth Factor-7-Functionalized Sericin Biomaterial Applicable for the Cultivation of Keratinocytes

Growth factors, including fibroblast growth factor-7 (FGF-7), are a group of proteins that stimulate various cellular processes and are often used with carriers to prevent the rapid loss of their activities. Sericin with great biocompatibility has been investigated as a proteinaceous carrier to enha...

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Autores principales: Lian, Ai Ai, Yamaji, Yuka, Kajiwara, Kazuki, Takaki, Keiko, Mori, Hajime, Liew, Mervyn Wing On, Kotani, Eiji, Maruta, Rina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456417/
https://www.ncbi.nlm.nih.gov/pubmed/36077351
http://dx.doi.org/10.3390/ijms23179953
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author Lian, Ai Ai
Yamaji, Yuka
Kajiwara, Kazuki
Takaki, Keiko
Mori, Hajime
Liew, Mervyn Wing On
Kotani, Eiji
Maruta, Rina
author_facet Lian, Ai Ai
Yamaji, Yuka
Kajiwara, Kazuki
Takaki, Keiko
Mori, Hajime
Liew, Mervyn Wing On
Kotani, Eiji
Maruta, Rina
author_sort Lian, Ai Ai
collection PubMed
description Growth factors, including fibroblast growth factor-7 (FGF-7), are a group of proteins that stimulate various cellular processes and are often used with carriers to prevent the rapid loss of their activities. Sericin with great biocompatibility has been investigated as a proteinaceous carrier to enhance the stability of incorporated proteins. The difficulties in obtaining intact sericin from silkworm cocoons and the handling of growth factors with poor stability necessitate an efficient technique to incorporate the protein into a sericin-based biomaterial. Here, we report the generation of a transgenic silkworm line simultaneously expressing and incorporating FGF-7 into cocoon shells containing almost exclusively sericin. Growth-factor-functionalized sericin cocoon shells requiring simple lyophilization and pulverization processes were successfully used to induce the proliferation and migration of keratinocytes. Moreover, FGF-7 incorporated into sericin-cocoon powder exhibited remarkable stability, with more than 70% of bioactivity being retained after being stored as a suspension at 25 °C for 3 months. Transgenic sericin-cocoon powder was used to continuously supply biologically active FGF-7 to generate a three-dimensionally cultured keratinocyte model in vitro. The outcomes of this study propound a feasible approach to producing cytokine-functionalized sericin materials that are ready to use for cell cultivation.
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spelling pubmed-94564172022-09-09 A Bioengineering Approach for the Development of Fibroblast Growth Factor-7-Functionalized Sericin Biomaterial Applicable for the Cultivation of Keratinocytes Lian, Ai Ai Yamaji, Yuka Kajiwara, Kazuki Takaki, Keiko Mori, Hajime Liew, Mervyn Wing On Kotani, Eiji Maruta, Rina Int J Mol Sci Article Growth factors, including fibroblast growth factor-7 (FGF-7), are a group of proteins that stimulate various cellular processes and are often used with carriers to prevent the rapid loss of their activities. Sericin with great biocompatibility has been investigated as a proteinaceous carrier to enhance the stability of incorporated proteins. The difficulties in obtaining intact sericin from silkworm cocoons and the handling of growth factors with poor stability necessitate an efficient technique to incorporate the protein into a sericin-based biomaterial. Here, we report the generation of a transgenic silkworm line simultaneously expressing and incorporating FGF-7 into cocoon shells containing almost exclusively sericin. Growth-factor-functionalized sericin cocoon shells requiring simple lyophilization and pulverization processes were successfully used to induce the proliferation and migration of keratinocytes. Moreover, FGF-7 incorporated into sericin-cocoon powder exhibited remarkable stability, with more than 70% of bioactivity being retained after being stored as a suspension at 25 °C for 3 months. Transgenic sericin-cocoon powder was used to continuously supply biologically active FGF-7 to generate a three-dimensionally cultured keratinocyte model in vitro. The outcomes of this study propound a feasible approach to producing cytokine-functionalized sericin materials that are ready to use for cell cultivation. MDPI 2022-09-01 /pmc/articles/PMC9456417/ /pubmed/36077351 http://dx.doi.org/10.3390/ijms23179953 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lian, Ai Ai
Yamaji, Yuka
Kajiwara, Kazuki
Takaki, Keiko
Mori, Hajime
Liew, Mervyn Wing On
Kotani, Eiji
Maruta, Rina
A Bioengineering Approach for the Development of Fibroblast Growth Factor-7-Functionalized Sericin Biomaterial Applicable for the Cultivation of Keratinocytes
title A Bioengineering Approach for the Development of Fibroblast Growth Factor-7-Functionalized Sericin Biomaterial Applicable for the Cultivation of Keratinocytes
title_full A Bioengineering Approach for the Development of Fibroblast Growth Factor-7-Functionalized Sericin Biomaterial Applicable for the Cultivation of Keratinocytes
title_fullStr A Bioengineering Approach for the Development of Fibroblast Growth Factor-7-Functionalized Sericin Biomaterial Applicable for the Cultivation of Keratinocytes
title_full_unstemmed A Bioengineering Approach for the Development of Fibroblast Growth Factor-7-Functionalized Sericin Biomaterial Applicable for the Cultivation of Keratinocytes
title_short A Bioengineering Approach for the Development of Fibroblast Growth Factor-7-Functionalized Sericin Biomaterial Applicable for the Cultivation of Keratinocytes
title_sort bioengineering approach for the development of fibroblast growth factor-7-functionalized sericin biomaterial applicable for the cultivation of keratinocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456417/
https://www.ncbi.nlm.nih.gov/pubmed/36077351
http://dx.doi.org/10.3390/ijms23179953
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