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Presume Why Probiotics May Not Provide Protection in Inflammatory Bowel Disease through an Azoxymethane and Dextran Sodium Sulfate Murine Model

Recent studies have shown dysbiosis is associated with inflammatory bowel disease (IBD). However, trying to restore microbial diversity via fecal microbiota transplantation (FMT) or probiotic intervention fails to achieve clinical benefit in IBD patients. We performed a probiotic intervention on a s...

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Autores principales: Hu, Ming-Luen, Lian, Wei-Shiung, Wang, Feng-Sheng, Yang, Chao-Hui, Huang, Wan-Ting, Yang, Jing-Wen, Chen, I-Ya, Yang, Ming-Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456426/
https://www.ncbi.nlm.nih.gov/pubmed/36077084
http://dx.doi.org/10.3390/ijms23179689
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author Hu, Ming-Luen
Lian, Wei-Shiung
Wang, Feng-Sheng
Yang, Chao-Hui
Huang, Wan-Ting
Yang, Jing-Wen
Chen, I-Ya
Yang, Ming-Yu
author_facet Hu, Ming-Luen
Lian, Wei-Shiung
Wang, Feng-Sheng
Yang, Chao-Hui
Huang, Wan-Ting
Yang, Jing-Wen
Chen, I-Ya
Yang, Ming-Yu
author_sort Hu, Ming-Luen
collection PubMed
description Recent studies have shown dysbiosis is associated with inflammatory bowel disease (IBD). However, trying to restore microbial diversity via fecal microbiota transplantation (FMT) or probiotic intervention fails to achieve clinical benefit in IBD patients. We performed a probiotic intervention on a simulated IBD murine model to clarify their relationship. IBD was simulated by the protocol of azoxymethane and dextran sodium sulfate (AOM/DSS) to set up a colitis and colitis-associated neoplasm model on BALB/c mice. A single probiotic intervention using Clostridium butyricum Miyairi (CBM) on AOM/DSS mice to clarify the role of probiotic in colitis, colitis-associated neoplasm, gut microbiota, and immune cytokines was performed. We found dysbiosis occurred in AOM/DSS mice. The CBM intervention on AOM/DSS mice failed to improve colitis and colitis-associated neoplasms but changed microbial composition and unexpectedly increased expression of proinflammatory IL-17A in rectal tissue. We hypothesized that the probiotic intervention caused dysbiosis. To clarify the result, we performed inverse FMT using feces from AOM/DSS mice to normal recipients to validate the pathogenic effect of dysbiosis from AOM/DSS mice and found mice on inverse FMT did develop colitis and colon neoplasms. We presumed the probiotic intervention to some extent caused dysbiosis as inverse FMT. The role of probiotics in IBD requires further elucidation.
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spelling pubmed-94564262022-09-09 Presume Why Probiotics May Not Provide Protection in Inflammatory Bowel Disease through an Azoxymethane and Dextran Sodium Sulfate Murine Model Hu, Ming-Luen Lian, Wei-Shiung Wang, Feng-Sheng Yang, Chao-Hui Huang, Wan-Ting Yang, Jing-Wen Chen, I-Ya Yang, Ming-Yu Int J Mol Sci Article Recent studies have shown dysbiosis is associated with inflammatory bowel disease (IBD). However, trying to restore microbial diversity via fecal microbiota transplantation (FMT) or probiotic intervention fails to achieve clinical benefit in IBD patients. We performed a probiotic intervention on a simulated IBD murine model to clarify their relationship. IBD was simulated by the protocol of azoxymethane and dextran sodium sulfate (AOM/DSS) to set up a colitis and colitis-associated neoplasm model on BALB/c mice. A single probiotic intervention using Clostridium butyricum Miyairi (CBM) on AOM/DSS mice to clarify the role of probiotic in colitis, colitis-associated neoplasm, gut microbiota, and immune cytokines was performed. We found dysbiosis occurred in AOM/DSS mice. The CBM intervention on AOM/DSS mice failed to improve colitis and colitis-associated neoplasms but changed microbial composition and unexpectedly increased expression of proinflammatory IL-17A in rectal tissue. We hypothesized that the probiotic intervention caused dysbiosis. To clarify the result, we performed inverse FMT using feces from AOM/DSS mice to normal recipients to validate the pathogenic effect of dysbiosis from AOM/DSS mice and found mice on inverse FMT did develop colitis and colon neoplasms. We presumed the probiotic intervention to some extent caused dysbiosis as inverse FMT. The role of probiotics in IBD requires further elucidation. MDPI 2022-08-26 /pmc/articles/PMC9456426/ /pubmed/36077084 http://dx.doi.org/10.3390/ijms23179689 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hu, Ming-Luen
Lian, Wei-Shiung
Wang, Feng-Sheng
Yang, Chao-Hui
Huang, Wan-Ting
Yang, Jing-Wen
Chen, I-Ya
Yang, Ming-Yu
Presume Why Probiotics May Not Provide Protection in Inflammatory Bowel Disease through an Azoxymethane and Dextran Sodium Sulfate Murine Model
title Presume Why Probiotics May Not Provide Protection in Inflammatory Bowel Disease through an Azoxymethane and Dextran Sodium Sulfate Murine Model
title_full Presume Why Probiotics May Not Provide Protection in Inflammatory Bowel Disease through an Azoxymethane and Dextran Sodium Sulfate Murine Model
title_fullStr Presume Why Probiotics May Not Provide Protection in Inflammatory Bowel Disease through an Azoxymethane and Dextran Sodium Sulfate Murine Model
title_full_unstemmed Presume Why Probiotics May Not Provide Protection in Inflammatory Bowel Disease through an Azoxymethane and Dextran Sodium Sulfate Murine Model
title_short Presume Why Probiotics May Not Provide Protection in Inflammatory Bowel Disease through an Azoxymethane and Dextran Sodium Sulfate Murine Model
title_sort presume why probiotics may not provide protection in inflammatory bowel disease through an azoxymethane and dextran sodium sulfate murine model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456426/
https://www.ncbi.nlm.nih.gov/pubmed/36077084
http://dx.doi.org/10.3390/ijms23179689
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