Cargando…

Microfluidic Electroceuticals Platform for Therapeutic Strategies of Intervertebral Disc Degeneration: Effects of Electrical Stimulation on Human Nucleus Pulposus Cells under Inflammatory Conditions

The degeneration of an intervertebral disc (IVD) is a major cause of lower back pain. IVD degeneration is characterized by the abnormal expression of inflammatory cytokines and matrix degradation enzymes secreted by IVD cells. In addition, macrophage-mediated inflammation is strongly associated with...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Tae-Won, Kim, An-Gi, Lee, Kwang-Ho, Hwang, Min-Ho, Choi, Hyuk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456475/
https://www.ncbi.nlm.nih.gov/pubmed/36077518
http://dx.doi.org/10.3390/ijms231710122
_version_ 1784785824681820160
author Kim, Tae-Won
Kim, An-Gi
Lee, Kwang-Ho
Hwang, Min-Ho
Choi, Hyuk
author_facet Kim, Tae-Won
Kim, An-Gi
Lee, Kwang-Ho
Hwang, Min-Ho
Choi, Hyuk
author_sort Kim, Tae-Won
collection PubMed
description The degeneration of an intervertebral disc (IVD) is a major cause of lower back pain. IVD degeneration is characterized by the abnormal expression of inflammatory cytokines and matrix degradation enzymes secreted by IVD cells. In addition, macrophage-mediated inflammation is strongly associated with IVD degeneration. However, the precise pathomechanisms of macrophage-mediated inflammation in IVD are still unknown. In this study, we developed a microfluidic platform integrated with an electrical stimulation (ES) array to investigate macrophage-mediated inflammation in human nucleus pulposus (NP). This platform provides multiple cocultures of different cell types with ES. We observed macrophage-mediated inflammation and considerable migration properties via upregulated expression of interleukin (IL)-6 (p < 0.001), IL-8 (p < 0.05), matrix metalloproteinase (MMP)-1 (p < 0.05), and MMP-3 (p < 0.05) in human NP cells cocultured with macrophages. We also confirmed the inhibitory effects of ES at 10 μA due to the production of IL-6 (p < 0.05) and IL-8 (p < 0.01) under these conditions. Our findings indicate that ES positively affects degenerative inflammation in diverse diseases. Accordingly, the microfluidic electroceutical platform can serve as a degenerative IVD inflammation in vitro model and provide a therapeutic strategy for electroceuticals.
format Online
Article
Text
id pubmed-9456475
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-94564752022-09-09 Microfluidic Electroceuticals Platform for Therapeutic Strategies of Intervertebral Disc Degeneration: Effects of Electrical Stimulation on Human Nucleus Pulposus Cells under Inflammatory Conditions Kim, Tae-Won Kim, An-Gi Lee, Kwang-Ho Hwang, Min-Ho Choi, Hyuk Int J Mol Sci Article The degeneration of an intervertebral disc (IVD) is a major cause of lower back pain. IVD degeneration is characterized by the abnormal expression of inflammatory cytokines and matrix degradation enzymes secreted by IVD cells. In addition, macrophage-mediated inflammation is strongly associated with IVD degeneration. However, the precise pathomechanisms of macrophage-mediated inflammation in IVD are still unknown. In this study, we developed a microfluidic platform integrated with an electrical stimulation (ES) array to investigate macrophage-mediated inflammation in human nucleus pulposus (NP). This platform provides multiple cocultures of different cell types with ES. We observed macrophage-mediated inflammation and considerable migration properties via upregulated expression of interleukin (IL)-6 (p < 0.001), IL-8 (p < 0.05), matrix metalloproteinase (MMP)-1 (p < 0.05), and MMP-3 (p < 0.05) in human NP cells cocultured with macrophages. We also confirmed the inhibitory effects of ES at 10 μA due to the production of IL-6 (p < 0.05) and IL-8 (p < 0.01) under these conditions. Our findings indicate that ES positively affects degenerative inflammation in diverse diseases. Accordingly, the microfluidic electroceutical platform can serve as a degenerative IVD inflammation in vitro model and provide a therapeutic strategy for electroceuticals. MDPI 2022-09-04 /pmc/articles/PMC9456475/ /pubmed/36077518 http://dx.doi.org/10.3390/ijms231710122 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Tae-Won
Kim, An-Gi
Lee, Kwang-Ho
Hwang, Min-Ho
Choi, Hyuk
Microfluidic Electroceuticals Platform for Therapeutic Strategies of Intervertebral Disc Degeneration: Effects of Electrical Stimulation on Human Nucleus Pulposus Cells under Inflammatory Conditions
title Microfluidic Electroceuticals Platform for Therapeutic Strategies of Intervertebral Disc Degeneration: Effects of Electrical Stimulation on Human Nucleus Pulposus Cells under Inflammatory Conditions
title_full Microfluidic Electroceuticals Platform for Therapeutic Strategies of Intervertebral Disc Degeneration: Effects of Electrical Stimulation on Human Nucleus Pulposus Cells under Inflammatory Conditions
title_fullStr Microfluidic Electroceuticals Platform for Therapeutic Strategies of Intervertebral Disc Degeneration: Effects of Electrical Stimulation on Human Nucleus Pulposus Cells under Inflammatory Conditions
title_full_unstemmed Microfluidic Electroceuticals Platform for Therapeutic Strategies of Intervertebral Disc Degeneration: Effects of Electrical Stimulation on Human Nucleus Pulposus Cells under Inflammatory Conditions
title_short Microfluidic Electroceuticals Platform for Therapeutic Strategies of Intervertebral Disc Degeneration: Effects of Electrical Stimulation on Human Nucleus Pulposus Cells under Inflammatory Conditions
title_sort microfluidic electroceuticals platform for therapeutic strategies of intervertebral disc degeneration: effects of electrical stimulation on human nucleus pulposus cells under inflammatory conditions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456475/
https://www.ncbi.nlm.nih.gov/pubmed/36077518
http://dx.doi.org/10.3390/ijms231710122
work_keys_str_mv AT kimtaewon microfluidicelectroceuticalsplatformfortherapeuticstrategiesofintervertebraldiscdegenerationeffectsofelectricalstimulationonhumannucleuspulposuscellsunderinflammatoryconditions
AT kimangi microfluidicelectroceuticalsplatformfortherapeuticstrategiesofintervertebraldiscdegenerationeffectsofelectricalstimulationonhumannucleuspulposuscellsunderinflammatoryconditions
AT leekwangho microfluidicelectroceuticalsplatformfortherapeuticstrategiesofintervertebraldiscdegenerationeffectsofelectricalstimulationonhumannucleuspulposuscellsunderinflammatoryconditions
AT hwangminho microfluidicelectroceuticalsplatformfortherapeuticstrategiesofintervertebraldiscdegenerationeffectsofelectricalstimulationonhumannucleuspulposuscellsunderinflammatoryconditions
AT choihyuk microfluidicelectroceuticalsplatformfortherapeuticstrategiesofintervertebraldiscdegenerationeffectsofelectricalstimulationonhumannucleuspulposuscellsunderinflammatoryconditions